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Home > Semnas TPV 2020 > Wardhana

Pengembangan Biosensor Penyakit Surra (Trypanosoma evansi) berbasis Protein dengan Metode Differential Pulse Voltammetry

A H Wardhana, H Munawar, D H Sawitri, R Maryam

Abstract

Trypanosoma evansi masih menjadi penyebab utama penyakit trypanosomiasis di Indonesia, yang dikenal dengan nama Surra. Namun demikian, kelemahan dalam mendiagnosa yang tepat dari Card Agglutination Test for Trypanosomiasis/T. evansi (CATT/T. evansi), ELISA and PCR menyebabkan pengendalian surra di lapang masih menghadapi kendala. Untuk itu, diperlukan alternatif piranti diagnosis yang cepat, akurat dan mudah diaplikasikan, yaitu biosensor. Penelitian ini bertujuan untuk mengetahui kinerja biosensor surra (Trypanosoma evansi) dalam mengidentifikasi sampel serum positif dan negatif. Sumber protein diisolasi dari T. evansi yang dipropagasi. Protein diimobilisasi dengan reaksi carbodiimide pada permukaan sensor (elektrode) karbon sebagai material yang memiliki respon terrendah terhadap serum/antibodi anti T. evansi (persentase arus listrik/elektron < 90). Metode yang digunakan pada pengembangan biosensor ini adalah differential pulse voltammetry (DPV). Data yang diperoleh dinormalisasi dan dianalisis menggunakan program PSTrace 5.8. Hasil penelitian membuktikan bahwa positif dan negatif palsu sensor dideteksi pada pengenceran serum 2 dan 64 kali. Serum positif yang dianalisis dengan metode DPV (kisaran potential listrik – 0.2 - +1.0 V dengan tingkat kecepatan pembacaan 0,1 Vs-1) menunjukkan bahwa slope serum positif (1,84) lebih tinggi dibandingkan dengan sampel negatif (1,01) yang mengindikasikan sensitivitas biosensor terhadap Surra (T. evansi). Hasil ini dikonfirmasi dengan melakukan pengujian serologis pada sampel menggunakan (Card Agglutination Test for Trypanosomiasis/T. evansi – CATT/T. evansi). Hasil penelitian ini menunjukkan bahwa biosensor surra/T. evansi berbasis protein merupakan piranti yang menjanjikan untuk dikembangkan dalam mendeteksi surra pada ternak.

 Keywords

Protein; DPV; biosensor; Trypanosoma evansi; surra

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DOI: https://dx.doi.org/10.14334/Pros.Semnas.TPV-2020-p.844-857

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