Anthrax retrospective analysis in endemic areas using enzyme-linked immunosorbent assay (ELISA)

S Hardjoutomo, M.B Poerwadikarta


About the end of 1995, an infectious disease among buffaloes as well as human beings was reported to occur in the District of Ngada, East Nusatenggara . Specimens of death animals, namely a piece of bones, hide, soil samples as well as blood and exudate smears and sera of the local inhabitants were submitted to Balitvet, Bogor laboratory for diagnosis . Bacteriological examinations of those specimens revealed that B. Anthracis was the causative agent of the disease . Along the line, 40 humans sera comprised 20 sera of local exposed inhabitants and 20 sera of non-exposed outsider, examined by the antibody enzyme-linked immunosorbent assay (ELISA) confirmed the diagnosis . It was concluded that antibody ELISA is one of diagnosis tools for analyzing of an anthrax outbreak in the field .


Keywords : Anthrax, retrospective analysis, antibody ELISA; serum

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