Effect of Averrhoa bilimbi Fruit Filtrate and Shrimp Paste Mixture on Performance, Gut Microbes and Blood Profile of Broilers

Indri Mareta, Gilbert Nathaniel, Turrini Yudiarti, Endang Widiastuti, Hanny Indrat Wahyuni, Sugiharto Sugiharto


This study was aimed to evaluate effect of a mixture of Averrhoa bilimbi fruit filtrate and shrimp paste (Mysis sp.) on the growth performance, blood profile, selected intestinal bacterial number and pH value of broiler digestive tract. The mixture of A. bilimbi fruit filtrate and shrimp paste were incubated for 4 days and were then used in the experiment. For in vivo experiment, 40 day-old Lohmann broiler chicks were distributed randomly to two treatment groups, i.e., control (T1) and chickens given the mixture of 10% A. bilimbi fruit filtrate and shrimp paste in drinking water (T2). Body weight and feed intake were recorded weekly. At day 42, 2 birds from each pen (8 chicks per treatment group) were taken for blood and digesta collection. Internal organ weight and carcass traits were determined thereafter. Feed conversion ratio (FCR) was lower (P<0.05) on treatment group than control. Hemoglobin and mean corpuscular volume (MCV) of treatment group were higher (P<0.05) than that of control. Total cholesterol was higher (P<0.05) in treatment group than that in control. Total cecum coliform was lower (P<0.05) in treatment group than that in control. The pH values of the small intestinal segments (jejunum, ileum, cecum) was lower (P<0.05) in treatment group than that in control group. In conclusion, administration of the blends of A. bilimbi fruit filtrate and shrimp paste into drinking water improved FCR, increased hemoglobin and MCV values, decreased gut pH and cecal coliform of broiler chickens.


Averrhoa bilimbi L, Broiler Chicken, Filtrate, Performance, Shrimp Paste

Full Text:



Abudabos AM, Murshed MA, Qaid MM, Abdelrahman AG. 2016. Effect of probiotics on serum biochemical and blood constituents in chicken challenged with Salmonella enterica Subsp Typhimurium. Trop J Pharm Res. 15:461.

Adil S, Banday T, Ahmad Bhat G, Salahuddin M, Raquib M, Shanaz S. 2011. Response of broiler chicken to dietary supplementation of organic acids. J Cent Eur Agric. 12:498–508.

Amalia U, Sumardianto, Agustini TW. 2018. Characterization of Lactic Acid Bacteria (LAB) isolated from Indonesian shrimp paste (terasi). IOP Conf Ser Earth Environ Sci. 116:012049.

Asna AN, Noriham A. 2014. Antioxidant activity and bioactive components of oxalidaceae fruit extracts. Malaysian J Anal Sci. 18:116–126.

Dewi P, Ratih GA, Burhannuddin B, Sudarmanto G. 2019. In vitro inhibitory activity of ethanolic fruit extract from Averrhoa bilimbi L. against Streptococcus pyogenes Bacteria. Heal Notions. 3:13–17.

Dittoe DK, Ricke SC, Kiess AS. 2018. Organic acids and potential for modifying the avian gastrointestinal tract and reducing pathogens and disease. Front Vet Sci.

Hidayat M, Zuprizal Z, Sundari S, Kurniawati A, Wati AK, Kusmayadi A. 2017. The effect of liquid tumeric extract supplementation on carcass production and chemical quality of broiler meat. J Indones Trop Anim Agric. 42:6.

Jin LZ, Ho YW, Abdullah N, Jalaludin S. 2000. Digestive and bacterial enzyme activities in broilers fed diets supplemented with Lactobacillus cultures. Poult Sci. 79:886–891.

Kobayashi T, Kajiwara M, Wahyuni M, Kitakado T, Hamada-Sato N, Imada C, Watanabe E. 2003. Isolation and characterization of halophilic lactic acid bacteria isolated from “terasi” shrimp paste: A traditional fermented seafood product in Indonesia. J Gen Appl Microbiol. 49:279–286.

Kumar Y, Chisti B, Sing A, Masih H, Mishra S. 2013. Isolation and charactization of Lactobacillus for probiotik properties. Int J Pharma Bio Sci. 4:B11–B21.

Maeda M, Shibata A, Biswas G, Korenaga H, Kono T, Itami T, Sakai M. 2014. Isolation of Lactic Acid Bacteria from Kuruma shrimp (Marsupenaeus japonicus) intestine and assessment of immunomodulatory role of a selected strain as probiotic. Mar Biotechnol. 16:181–192.

Malik HEE, Hafzalla RHH, Ali OHA, Elhassan MMO, Dousa BM, Ali AM, Elamin KM. 2016. Effect of probiotics and acidifiers on carcass yield internal organs cuts and meat to bone ratio of broiler chicken. J Agric Vet Sci. 9:18–23.

Marín-Flamand E, Vázquez-Durán A, Méndez-Albores A. 2013. Effect of organic acid blends in drinking water on growth performance, blood constituents and immune response of broiler chickens. J Poult Sci. 51:144–150.

Nakyinsige K, Abdul Rahman NS, Salwani MS, Abd Hamid A, Adeyemi KD, Sakimin SZ, Sazili AQ. 2016. Effect of belimbing buluh (Averrhoa bilimbi) juice extract on oxidative stability and microbiological quality of spent chicken meat. Int Food Res J. 23:2675–2680.

Ndelekwute E, Enyenihi G, Unah U, Madu H. 2016. Dietary effects of different organic acids on growth and nutrient digestibility of broiler. Bangladesh J Anim Sci. 45:10–17.

Octavia F, Isroli, Wahyuni HI. 2018. Pengaruh penambahan air perasan jeruk nipis (Citrus aurantiifolia) dalam air minum sebagai acidifier terhadap profil lemak darah ayam broiler. J Ilmu dan Teknol Peternak. 6:92–96.

Patil AG, Koli SP, Patil DA. 2013. Pharmcognostical standardization and HPTLC fingerprint of Averrhoa bilimbi (L.) fruits. J Pharm Res. 6:145–150.

Saleh AA, Amber KA, Mousa MM, Nada AL, Awad W, Dawood MAO, Abd El-Moneim AE-ME, Ebeid TA, Abdel-Daim MM. 2020. A mixture of exogenous emulsifiers increased the acceptance of broilers to low energy diets: growth performance, blood chemistry, and fatty acids traits. Animals. 10:437.

Salgado-Tránsito L, Del Río-García J, Arjona-Román J, Moreno-Martínez E, Méndez-Albores A. 2011. Effect of citric acid supplemented diets on aflatoxin degradation, growth performance and serum parameters in broiler chickens. Arch Med Vet. 43:215–222.

Singh S, Shukla S, Tandia N, Kumar N, Paliwa R. 2014. Antibiotic residues a global challenge. Pharma Sci Monit. 5:184–197.

Sudjana. 1989. Metode Statistika. 5th ed. Bandung (Indones): Tarsito.

Sugiharto S. 2016. Role of nutraceuticals in gut health and growth performance of poultry. J Saudi Soc Agric Sci.

Sugiharto S, Isroli I, Yudiarti T, Widiastuti E. 2018. The effect of supplementation of multistrain probiotic preparation in combination with vitamins and minerals to the basal diet on the growth performance, carcass traits, and physiological response of broilers. Vet World. 11:240–247.

Sugiharto S, Yudiarti T, Isroli I, Widiastuti E, Putra FD. 2017. Effect of dietary supplementation with Rhizopus oryzae or Chrysonilia crassa on growth performance blood profile intestinal microbial population and carcass traits in broiler exposed to heat stress. Arch Anim Breed. 60:347–356.

Sumarsih S, Sulistiyanto B, Sutrisno CI. 2012. Characteristics, stability and antimicrobial activity of lactic acid bacteria (Leuconostoc sp) isolated from broiler’s caecum during storage. J Indones Trop Anim Agric. 37:70–76.

Surai PF. 2016. Antioxidant systems in poultry biology: superoxide dismutase. J Anim Res Nutr. 01:1–17.

Taherpour K, Moravej H, Shivazad M, Adibmoradi M, Yakhchali B. 2009. Effects of dietary probiotic, prebiotic and butyric acid glycerides on performance and serum composition in broiler chickens. African J Biotechnol. 8:2329–2334.

Tosi G, Massi P, Antongiovanni M, Buccioni A, Minieri S, Marenchino L, Mele M. 2013. Efficacy test of a hydrolysable tannin extract against necrotic enteritis in challenged broiler chickens. Ital J Anim Sci. 12:e62.

Ugwuene MC. 2011. Effect of dietary palm kernel meal for maize on the haematological and serum chemistry of broiler turkey. Niger J Anim Sci. 13:93–103.

Yakhkeshi S, Rahimi S, Gharib Naseri K. 2011. The effects of comparison of herbal extracts, antibiotic, probiotic and organic acid on serum lipids, immune response, GIT microbial population, intestinal morphology and performance of broilers. J Med Plants. 10:37.

Youssef IMI, Mostafa AS, Abdel-Wahab MA. 2017. Effects of dietary inclusion of probiotics and organic acids on performance, intestinal microbiology, serum biochemistry and carcass traits of broiler chickens. J World’s Poult Res. 7:57–71.


  • There are currently no refbacks.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.