Effectivity of BS4 enzyme complex on the performance of laying hens fed with different ingredients

Arnold P. Sinurat, Tresnawati Purwadaria, Tuti Haryati


An experiment was carried out to evaluate the effectivity of an enzyme complex produced by Eupenicilium javanicum BS4 on the performance of laying hens fed with different feed ingredients. Three diets were formulated with similar nutrients (protein, ME, digestible amino acids, Ca and available P) value to meet the nutrient requirement of laying hens.  Diets were formulated based on maize, palm kernel cake (PKC) or rice bran.  The diets were either supplemented or not with BS4 enzyme complex and fed to Isa Brown pullet from 19 to 37 weeks old. Each diet was fed to 24 birds and the performances were recorded. Data were analysed statistically with 2 X 3 factorial design with six replicates. Results showed that supplementation of BS4 enzyme into laying hens diet did not influence body weight change, feed intake, mortalities, egg weight and egg shell thickness but significantly increased egg production (P<0.05) and the FCR (P<0.01) in any feedstuff tested. It also improved egg yolk colour score on diet contained 20% PKC. Laying hens fed 20% PKC produced more eggs than those fed maize or rice bran. The egg shell of laying hens fed rice bran were thicker than those fed with maize or PKC. It is concluded that BS4 enzyme complex is effective when supplemented into laying hens diet.


BS4 Enzyme; Palm Kernel Cake; Rice Bran; Egg Production; Egg Quality

Full Text:



Abeyrathna HMWN, Atapattu NSBM, Gunawardane WWDA. 2014. Effects of the level of dietary rice bran with or without phytase on performance and egg parameters of laying Japanese quail. Trop Agric Res. 26:39-47.

Abubakar A, Tukur HM, Sekoni AA, Hassan WA. 2007. Performance and egg quality characteristics of laying birds fed diets containing rice bran with and without yeast supplementation. Asian J Anim Sci. 1:1-9.

Batal A, Dale N. 2012. Ingredient Analysis Table: 2011 Edition. Feedstuffs, 15 September 2010. https://fdsmagissues.feedstuffs.com [Internet]. [cited 2014 Aug 9]. Available from: https://fdsmagissues.feedstuffs.com.

Bedford MR. 2000. Mechanism of action and potential environmental benefits from the use of feed enzymes. Anim Feed Sci Technol. 53:145-155.

Choct M, Derrsjant-Li Y, McLeish J, Peisker M. 2010. Soy oligosaccharides and soluble Non-starch polysaccharides: A Review of digestion, nutritive and anti-nutritive effects in pigs and poultry. Asian-Aust J Anim Sci. 23:1386-1398.

Chong CH, Zulkifli I, Blair R. 2008. Effects of dietary inclusion of palm kernel cake and palm oil and enzyme supplementation on performance of laying hens. Asian-Aust J Anim Sci. 21:1053-1058.

Gallinger CI, Suarez DM, Irazusta A. 2004. Effects of rice bran inclusion on performance and bone mineralization in broiler chicks. J Appl Poult Res. 13:183-190.

Haryati T, Purwadaria T, Darma J, Tangendjaja B. 1997. Production of extracellular glycosidase by Eupenicillium javanicum and Aspergillus niger NRRL 337 on the coconut meal substrate. In: Proceeding Second Conf Agric Biotechnol. Jakarta (Indones): Indonesian Agency for Agricultural Research and Development. p. 517-522.

Hendrix Genetics. 2007. Performance of Isa Brown Final Product.

Jahromi MF, Liang JB, Abdullah N, Goh YM, Ebrahimi R, Shokryazdan P. 2016. Extraction and characterization of oligosaccharides from palm kernel cake as prebiotic. Bio Resour. 11:674-695.

Knudsen KEB. 1997. Carbohydrate and lignin contents of plant materials used in animal feeding. Anim Feed Sci Technol. 67:319-338.

Narvaez-Solarte W, Rostagno HS, Soares PR, Silva MA, Velasquez LFU. 2005. Nutritional requirements in methionine + Cystine for White- egg laying hens during the first cycle of production. Int J Poult Sci. 4:965-968.

Navidshad B, Liang JB, Jahromi MF, Akhlaghi A, Abdullah N. 2015. A comparison between a yeast cell wall extract (Bio-Mos®) and palm kernel expeller as mannan-oligosac-charides sources on the performance and ileal microbial population of broiler chickens. Ital J Anim Sci. 14:3452.

Pasaribu P, Sinurat AP, Purwadaria T, Ketaren PP. 2009. Peningkatan nilai gizi solid heavy phase sebagai pengganti jagung dalam pakan unggas. JITV. 14:167-176.

Perez JF, Gernat AG, Murillo JG. 2000. The effect of different levels of palm kernel meal in layer diets. Poult Sci. 79:77-79.

Rakhmani SIW, Pangestu Y, Sinurat AP, Purwadaria T. 2015. Carbohydrate and protein digestion on palm kernal cake by Mannanase BS4 and papain cocktail enzymes. Indones J Anim Vet Sci. 20:268-274.

Ravindran V. 2013. Feed enzymes: The science, practice, and metabolic realities. J Appl Poult Res. 22:628-636.

Saenphoom P, Liang JB, Ho YW, Loh TC, Rosfarizan M. 2013. Effects of enzyme treated palm kernel expeller on metabolizable energy, growth performance, villus height and digesta viscosity in broiler chickens. Asian-Aust J Anim Sci. 26:537-544.

Samli HE, Senkoylu N, Akyurek H, Agma A. 2006. Using rice bran in laying hen diets. Cent Eur Agric. 7:135-140.

Sinurat AP, Purwadaria T, Bintang IAK, Pasaribu T, Manurung, B P Manurung N. 2008. Substitution of corn with enzymes treated palm oil sludge in laying hens diet. In: Procedings XXIII World’s Poult Sci Congr. Brisbane (Australia).

Sinurat AP, Purwadaria T, Pasaribu T. 2013. Peningkatan nilai gizi bungkil inti sawit dengan pengurangan cangkang dan penambahan enzim. Indones J Anim Vet Sci. 18:34-41.

Sinurat AP, Purwadaria T, Pasaribu T, Ketaren PP. 2011. Performances of laying hens fed with enzyme supplemented palm kernel cake diets. In: Procedings 9th Asia Pacific Poult Conf. Taipeh (Taiwan).

Sundu B, Kumar A, Dingle J. 2006. Palm kernel meal in broiler diets: Effect on chicken performance and health. World Poult Sci J. 62:316-325.

Utami W, Meryandini A, Wiryawan KG. 2013. Characterization of bacterial mannanase for hydrolyzing palm kernel cake to produce manno-oligosaccharides prebiotics. Media Peternak. 36:192-196.

Zanu HK, Abangiba J, Arthur-Badoo W, Akparibo AD, Sam R. 2012. Laying chickens’ response to various levels of palm kernel cake in diets. Int J Livest Prod. 3:12-16.

Zita L, Tumova E, Stolc L. 2009. Effects of genotype, age and their interaction on egg quality in brown-egg laying hens. Acta Vet BRNO. 78:85-91.


  • There are currently no refbacks.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.