Carbohydrate and protein digestion on palm kernal cake by Mannanase BS4 and papain cocktail enzymes

SIW Rakhmani, Y. Pangestu, Arnold P. Sinurat, Tresnawati Purwadaria

Abstract

Supplementation of the mannanase BS4 enzyme on palm kernel cake (PKC) increased its metabolisable energy (ME), and supplementation with protease is expected to increase its protein digestibility. Therefore, the purpose of this research is to determine the optimum proportion of cocktail enzymes between BS4 β-mannanase (produced by Eupenicillium javanicum) and protease (papain) and their degradation activities on carbohydrate and protein of PKC. The β-Mannanase was produced by the mold through solid substrate fermentation (SSF) using coconut meal as the substrate. The papain was extracted from papaya latex (PL), collected by longitudinal incisions on unripe papaya fruit and oven dried overnight. The evaluation of enzyme cocktails for PKC hydrolysis was done at pH 5.8 and 40°C which are similar with poultry intestine condition and both enzymes are still active. The β- mannanase BS4 : papain were mixed with some proportions, i.e.: 100 : 0; 75 : 25; 50 : 50; 25 : 75 and 0 : 100% (by volume) in order to study the optimum cocktail composition ratio. The activities of β-mannanase towards gum locust bean was 86 U.ml-1 and papains PL activity was 18,000 U.g-1respectively. PL was chosen for synergistic reaction and compared with a commercial Merck papain (CMP, 20,000 U.g-1) as positive control. β-Mannanase BS4 showed carbohydrate digestion activity, and protein digestion activity was not detected. Papain showed protein digestion activity and no carbohydrate digestion activity. Enzyme cocktails of 50 : 50 from PL protease showed slightly increased in synergistic protein digestion activity in PKC. However, its reduction sugar production was much lower than 100 : 0 and 75 : 25. Amino acids production by enzyme cocktails 75 : 25 were higher than that of 100 : 0. As a result, the best volume composition of β-mannanase BS4 and papaya latex was 75 : 25 (v:v) or 14 : 10 (U:U).

Key Words: Mannanase BS4, Papain, Cocktail-Enzymes, Palm Kernel Cake

Keywords

Mannanase BS4; Papain; Cocktail-Enzymes; Palm Kernel Cake

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References

Alimon AR. 2004. The nutritive value of palm kernel cake for animal feed. Palm Oil Develop. 40:12-14.

Anson ML. 1938. The estimation of pepsin, trypsin, papain and cathepsin with hemoglobin. J Gen Physiol. 22:79-89.

Chong CH, Zulkifli I, Blair R. 2008. Effects of dietary inclusion of palm kernel cake and palm oil, and enzyme supplementation on performance of laying hens. Asian-Aust J Anim Sci. 21:1053-1058.

Cowieson AJ, Ravindran V. 2008. Effect of exogenous enzymes in maize-based diets varying in nutrient density for young broilers: growth performance and digestibility of energy, minerals and amino acids. Br Poult Sci. 49:37-44. doi: 10.1080/00071660701812989 18210288.

Cowieson AJ. 2010. Strategic selection of exogenous enzymes for corn/soy-based diets. J Poult Sci. 47:1-7. doi: 10.2141/jpsa.009045

Dusterhoft E, Voragen AGJ, Engels FM. 1991. Non-starch polysaccharides from sunflower (Helianthus annuus) meal and palm kernel (Elaeis guineenis) meal-preparation of cell wall material and extraction of polysaccharide fractions. J Sci Food Agric. 55:411-422.

Ezekiel A, Mamboya F. 2012 Papain, a plant enzyme of biological importance: A review. Am J Biochem Biotechnol. 8:99-104.

Ezieshi EV, Olomu JM. 2007. Nutritional evaluation of palm kernel meal types: 1. Proximate composition and metabolizable energy values. Afr J Biotechnol. 6:2484-2486.

Gilbert HJ, Stalbrand H, Brumer H.2008. How the walls come crumbling down: Recent structural biochemistry of plant polysaccharide degradation. Curr Opin Plant Biol. 11:338-348.

Giloh M, Shinder D, Yahav S. 2012. Skin surface temperature of broiler chickens is correlated to body core temperature and is indicative of their thermoregulatory status. Poult Sci. 91:175-188.

Mabelebele OJ, Alabi JW, Ng`ambi, Norris D, Ginindza MM. 2014. Comparison of gastrointestinal tracts and pH values of digestive organs of Ross 308 broiler and indigenous Venda chickens fed the same diet. Asian J Anim Vet Adv. 9:71-76.

Mandels M, Reese ET. 1957. Induction of cellulase in Trichoderma viride as influenced by carbon source and metals. J Bacteriol. 73:269-278.

McGrath ME. 1999. The lysosomal cysteine proteases. Ann Rev Biophys Biomol Struct. 28:181-204.

Meng X, Slominski BA. 2005. Nutritive values of corn, soybean meal, canola meal, and peas for broiler chickens as affected by a multicarbohydrase preparation of cell wall degrading enzymes. Poult Sci. 84:1242-1251.

Miller GL. 1959. Using of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem. 31:426-428.

Nitsawang S, Hatti-Kaul R, Kanasawud P. 2006. Purification of papain from Carica papaya latex: Aqueous two-phase extraction versus two-step salt precipitation. Enzyme Microbial Technol. 39:1103-1107.

Omar MA, Hamdan AM. 1998. Palm oil production. Jung JK, editor. Proceedings of the 8th World Congress on Animal Production, Pre-conference Symposia. p. 306-312. Seoul (Korea): Seoul National University Press.

Pasaribu T, Sinurat AP, Purwadaria T, Ketaren PP. 2009. Improving the nutritive values of solid heavy phase to substitute corn in laying hens diet. JITV. 14:167-176.

Ravindran V. 2013. Feed enzymes: The science, practice, and metabolic realities. J Appl Poult Res. 22:628-636.

Sinurat AP, Purwadaria T, Ketaren PP, Pasaribu T. 2014. Substitutions of soybean meal with enriched palm kernel meal in laying hens diet. JITV. 19:184-192. doi: https://dx.doi.org/10.14334/jitv.v19i3.1081

Slominski BA. 2011. Recent advances in research on enzymes for poultry diets. Poult Sci. 90:2013-2023. doi: 10.3382/ps.2011-01372 21844268

Slominski BA, Meng X, Campbell LD, Guenter W, Jones O. 2006. The use of enzyme technology for improved energy utilisation from full-fat oilseeds. Part II: Flaxseed. Poult Sci. 85:1031-1037.

Soltan MA. 2009. Growth performance, immune response and carcass traits of broiler chicks fed on graded level of palm kernel cake without or with enzyme supplementation. Livest Res Rur Develop. 21. https://www.lrrd.org/lrrd21/3/solt21037.htm

Sundu B, Kumar A, Dingle J. 2006. Palm kernel meal in broiler diets: Its effect on chicken performance and health. World’s Poult Sci J. 62:316-325.

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