DNA Amplification Technique for Detection of Bovine Brucellosis

Susan Maphilindawati Noor


Brucellosis in cattle is one of the important diseases that must be controlled since it causes significant economic losses and zoonotic disease. Early detection of the disease is essential to prevent the spread of brucellosis in livestock and humans. The success of brucellosis control relies on detection methods that are fast, sensitive and specific. Detection of bovine brucellosis in Indonesia still depends on conventional methods through serological tests and bacterial cultures. Currently, molecular techniques with DNA amplification have been widely developed and applied in many countries for the detection of brucellosis in livestock and humans. Although conventional methods have been widely used for laboratory diagnosis, molecular techniques has proved to be useful due to its specificity, sensitivity and speed in detecting Brucella sp especially on blood samples, milk and cement that are very difficult to detect by bacterial culture. Various molecular detection methods for the diagnosis of bovine brucellosis has been developed which include polymerase chain reaction (real-time PCR, multiplex PCR), Loop-mediated isothermal amplificatiom (LAMP), finger printing, and restriction fraction length polymorphism (RFLP).


Brucellosis; bovine; detection; molecular; DNA


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