The Use of Bacteriophage for Detection and Biocontrol of Foodborne Pathogen

Tati Ariyanti


Bacteriophages are viruses that have ability to attack bacterial cells in specific receptors, infect, multiply in bacterial cells and eventually lyse bacterial cells. This unique bacteriophage character is highly beneficial because it is harmless to mammalian cells and does not interfere with natural microbes. Bacteriophages are easy to obtain because they are widespread in the environment such as soil, water, animal, and farm waste or food. This paper describes the potential use of bacteriophages to detect pathogen and foodborne pathogen biocontrol. Bacteriophages are very potential to control the growth of pathogenic bacteria both in food industry and environment. Bacteriophages act as antibiotics, detection tool for pathogenic bacteria in the food chain, food biopreservative from pathogen bacteria contamination, and foodborne disease prevention. Although research on bacteriophage in Indonesia has not been widely reported, research on bacteriophage utilization is being carried on.


Bacteriophage; foodborne disease; antibiotics; pathogen bacteria

Full Text:



Ackermann HW. 2005. Bacteriophage classification. In: Kutter, Elizabeth, Sulakvelidze A, editors. Bacteriophage, biology and applications. Washington DC (US): CRC Press. p. 29-66.

Bach SJ, McAllister TA, Veira DM, Gannon VPJ, Holley RA. 2003. Effect of bacteriophage DC22 on Escherichia coli O157:H7 in an artificial rumen system (rusitec) and inoculated sheep. Anim Res. 52:89-101.

Bielke LR, Tellez G, Hargis BM. 2012. Successes and failures of bacteriophage treatment of Enterobacteriaceae Infections in the gastrointestinal tract of domestic animals. In: Kurtboke I, editor. Bacteriophages [Internet]. Rijeka (Croatia): InTech. p. 159-178. Available from: https://www.intechopen. com/books/bacteriophages/successes-and-failures-of-bacteriophage-treatment-of-enterobacteriaceae-infections-in-the-gastrointe

Callaway TR, Edrington TS, Brabban AD, Anderson RC, Rossman ML, Engler MJ, Carr MA, Genovese KJ, Keen JE, Looper ML, et al. 2008. Bacteriophage isolated from feedlot cattle can reduce Escherichia coli O157:H7 populations in ruminant gastrointestinal tracts. Foodborne Pathog Dis. 5:183-191.

Coffey B, Rivas L, Duffy G, Coffey A, Ross RP, McAuliffe O. 2011. Assessment of Escherichia coli O157:H7-specific bacteriophages e11/2 and e4/1c in model broth and hide environments. Int J Food Microbiol. 147:188-194.

Garcia P, Martinez B, Obeso JM, Rodriguez A. 2008. Bacteriophages and their application in food safety. Lett Appl Microbiol. 47:479-485.

Goodridge L, Gallaccio A, Griffiths MW. 2003. Morphological, host range, and genetic characterization of two coliphages. Appl Environ Microbiol. 69:5364-5371.

Goodridge LD, Bisha B. 2011. Phage-based biocontrol strategies to reduce foodborne pathogens in foods. Bacteriophage. 1:130-137.

Grabow WOK. 2001. Bacteriophages: Update on application as models for viruses in water. Water SA. 27:251-268.

Greer GG. 2005. Bacteriophage control of foodborne bacteria. J Food Prot. 68:1102-1111.

Hagens S, Loessner MJ. 2007. Application of bacteriophages for detection and control of foodborne pathogens. Appl Microbiol Biotechnol. 76:513-519.

Higgins JP, Higgins SE, Guenther KL, Huff W, Donoghue AM, Donoghue DJ, Hargis BM. 2005. Use of a specific bacteriophage treatment to reduce Salmonella in poultry products. Poult Sci. 84:1141-1145.

Kudva IT, Jelacic S, Tarr PI, Youderian P, Hovde CJ. 1999. Biocontrol of Escherichia coli O157 with O157-specific bacteriophages. Appl Environ Microbiol. 65:3767-3773.

Lee HS. 2009. Somatic coliphage families as potential indicators of enteric viruses in water and methods for their detection [Dissertation]. [Chapel Hill (US)]: University of North Carolina.

O’Flynn G, Ross RP, Fitzgerald GF, Coffey A. 2004. Evaluation of a cocktail of three bacteriophages for biocontrol of Escherichia coli O157:H7. Appl Environ Microbiol. 70:3417-3424.

Poernomo S, Priadi A, Natalia L. 2006. Phage typing dan uji sensitivitas terhadap berbagai antibiotika dari isolat Salmonella enteritidis asal Indonesia. JITV. 11:157-166.

Rees C, Botsaris G. 2012. The use of phage for detection, antibiotic sensitivity testing and enumeration. In: Cardona PJ, editor. Understanding tuberculosis - Global experiences and innovative approaches to the diagnosis. Rijeka (Croatia): InTech. p. 293-306.

Rees C, Loessner M. 2005. Phage for detection of pathogenic bacteria. In: Elizabeth KSA, editor. Bacteriophage, biology and applications. Florida (US): CRC Press. p. 267-284.

Sajjad M, Rahman SU, Hussain I, Rasool MH. 2004. Application of coliphage lysate: A preliminary trial to treat an experimental Escherichia coli infection in broiler chicken. Int J Poult Sci. 3:538-542.

Santos SB, Carvalho CM, Sillankorva S, Nicolau A, Ferreira EC, Azeredo J. 2009. The use of antibiotics to improve phage detection and enumeration by the double-layer agar technique. BMC Microbiol. 9:1-10.

Sharma M, Patel JR, Conway WS, Ferguson S, Sulakvelidze A. 2009. Effectiveness of bacteriophages in reducing Escherichia coli O157:H7 on fresh-cut cantaloupes and lettucet. J Food Prot. 72:1481-1485.

Sheng H, Knecht HJ, Kudva IT, Hovde CJ. 2006. Application of bacteriophages to control intestinal Escherichia coli O157:H7 levels in ruminants. Appl Environ Microbiol. 72:5359-5366.

Shin H, Lee JH, Kim H, Choi Y, Heu S, Ryu S. 2012. Receptor diversity and host interaction of bacteriophages infecting Salmonella enterica serovar Typhimurium. PLoS One. 7:e43392.

Sillankorva SM, Oliveira H, Azeredo J, Sillankorva SM, Oliveira H, Azeredo J. 2012. Bacteriophages and their role in food safety. Int J Microbiol. 2012:1-13.

Tiwari R, Dhama K, Chakraborty S, Kumar A, Rahal A, Kapoor S. 2014. Bacteriophage therapy for safeguarding animal and human health: A review. Pakistan J Biol Sci. 17:301-315.

Tomat D, Mercanti D, Balagué C, Quiberoni A. 2013. Phage biocontrol of enteropathogenic and shiga toxin-producing Escherichia coli during milk fermentation. Lett Appl Microbiol. 57:3-10.

Viazis S, Akhtar M, Feirtag J, Diez-Gonzalez F. 2011. Reduction of Escherichia coli O157:H7 viability on leafy green vegetables by treatment with a bacteriophage mixture and trans-cinnamaldehyde. Food Microbiol. 28:149-157.

Yoichi M, Abe M, Miyanaga K, Unno H, Tanji Y. 2005. Alteration of tail fiber protein gp38 enables T2 phage to infect Escherichia coli O157:H7. J Biotechnol. 115:101-107.

You L, Suthers PF, Yin J. 2002. Effects of Escherichia coli physiology on growth of phage T7 in vivo and in silico. J Bacteriol. 184:1888-1894.


  • There are currently no refbacks.

Copyright (c)  2018 WARTAZOA. Indonesian Bulletin of Animal and Veterinary Sciences

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.