Development and Conservation of Gonadal Primordial Germ Cells for Preservation of Local Chicken in Indonesia

Tatan Kostaman, S Sopiyana

Abstract

One of the ex situ conservation techniques for poultry that recently developed was to collect primordial germ cell (PGC) or gonadal primordial germ cell (gPGC) that isolated from embryo development. Primordial germ cells (PGC) are embryonic cells that migrate to the gonads and form the precursors of gametes. The unique nature and accessibility of PGC during the early development provides an opportunity to manipulate the poultry germplasm, for example by forming germline chimeras. There are some stages that must be done through isolation and collection of PGC from its resources i.e. blastoderm, embryonic circulation blood and gonad. PGC collection originating from the gonads is one of existing PGC resources and technologies. gonadal PGC have advantages compared with other sources, namely (1) A large number of gonadal PGC can be taken from an embryo; and (2) A collection of gonadal PGC can be used in developing management systems of local avian germplasm conservation. This review is intended to describe the usefulness of isolation and collection technology of gonadal PGC as the local poultry germplasm conservation in Indonesia.

Keywords

Collection; gonadal primordial germ cell; native chicken

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References

Alvarenga MA, Papa FO, Landim-Alvarenga FC, Medeiros ASL. 2005. Amides as cryoprotectants for freezing stallion semen: A review. Anim Reprod Sci. 89:105-113.

Aramaki S, Kubota K, Soh T, Yamauchi N, Hattori MA. 2009. Chicken dead end homologue protein is a nucleoprotein of germ cells including primordial germ cells. J Reprod Dev. 55:214-218.

Aramaki S, Sato F, Kato T, Soh T, Kato Y, Hattori MA. 2007. Molecular cloning and expression of dead end homologue in chicken primordial germ cells. Cell Tissue Res. 330:45-52.

Basu S, Campbell HM, Dittel BN, Ray A. 2010. Purification of specific cell population by fluorescence activated cell sorting (FACS). J Vis Exp. 41:1-4.

Best B. 1990. Viability, cryoprotectant toxicity and chilling injury in cryogenics. BenBest [Internet]. [cited 1 May 2013]. Available from: http://www.benbest.com/pdf

Carlon N, Stahl A. 1985. Origin of the somatic components in chick embryonic gonads. Arch Anat Microsc Morphol Exp. 74:52-59.

Chalah T, Seigneurin F, Bleisbos E, Brillard JP. 1999. In vitro comparison of fowl sperm viability in ejaculates frozen by three different techniques and relationship with subsequent fertility in vivo. Cryobiology. 39:185-191.

Chojnacka-Puchta L, Kasperczyk K, Plucienniczak G, Sawicka D, Bednarczyk M. 2012. Primordial germ cells (PGCs) as a tool for creating transgenic chickens. Pol J Vet Sci. 15:181-188.

D’Costa S, Pardue SL, Petitte JN. 2001. Comparative development of avian primordial germ cells and production of germ line chimeras. Avian Poult Biol Rev. 12:151-168.

Ginsburg M. 1997. Primordial germ cell development in avians. Poult Sci. 76:91-95.

Glover JD, McGrew MJ. 2012. Primordial germ cell technologies for avian germplasm cryopreservation and investigating germ cell development. J Poult Sci. 49:155-162.

Hamburger V, Hamilton HL. 1951. A series of normal stages in the development of the chick embryo. J Morphol. 88:45-92.

Hammerstedt RH. 1995. Cryopreservation of poultry semen-current status and economics. In: Bakst MR, Wishart GJ, editors. Proceeding 1st International Symposium on the Artificial Insemination of Poultry. Savoy (US): Poultry Science Association. p. 229-250.

Han JY, Park TS, Hong YH, Jeong DK, Kim JN, Kim KD, Lim JM. 2002. Production of germline chimeras by transfer of chicken gonadal primordial germ cells maintained in vitro for an extended period. Theriogenology. 58:1531-1539.

Kang SJ, Choi JW, Park KJ, Lee YM, Kim TM, Sohn SH, Lim JM, Han JY. 2009. Development of a pheasant interspecies primordial germ cell transfer to chicken embryo: Effect of donor cell sex on chimeric semen production. Theriogenology. 72:519-527.

Kasperczyk K, Bajek A, Joachimiak R, Walasik K, Marszalek A, Drewa T, Bednarczyk M. 2012. In vitro optimization of the Gallus domesticus oviduct epithelial cells culture. Theriogenology. 77:1834-1845.

Kim H, Kim DH, Han JY, Choi SB, Ko YG, Do YJ, Seong HH, Kim SW. 2013a. The effect of modified cryopreservation method on viability of frozen-thawed primordial germ cell on the Korean native chicken (Ogye). J Anim Sci Technol. 55:427-434.

Kim H, Kim DH, Han JY, Do YJ, Kim JH, Kim YS, Seong HH, Ko YG, Kim SW. 2013b. Cryopreservation of primordial germ cells (PGCs) from Korean native chicken (Ogye) embryos using commercial cryoprotectants. Korean J Poult Sci. 40:163-169.

Kim MA, Park TS, Kim JN, Park HJ, Lee YM, Ono T, Lim JM, Han JY. 2005. Production of quail (Coturnix japonica) germline chimeras by transfer of gonadal primordial germ cells into recipient embryos. Theriogenology. 63:774-782.

Kito G, Aramaki S, Tanaka K, Soh T, Yamauchi N, Hattori M. 2010. Temporal and spatial differential expression of chicken germline-specific proteins cDAZL, CDH and CVH during gametogenesis. J Reprod Dev. 56:341-346.

Kobayashi T, Takeuchi Y, Yoshizaki G, Takeuchi T. 2003. Cryopreservation of trout primordial germ cells. Fish Physiol Biochem. 28:479-480.

Kohara Y, Kanai Y, Tajima A. 2008. Cryopreservation of gonadal germ cells (GGCs) from the domestic chicken using vitrification. J Poult Sci. 45:57-61.

Kostaman T. 2013. Isolasi dan kriopreservasi primordial germ cells (PGCs) menggunakan krioprotektan DMSO untuk pembentukan germline chimera ayam Gaok [Disertasi]. [Bogor (Indonesia)]: Institut Pertanian Bogor.

Kostaman T, Setioko AR. 2011. Perkembangan penelitian teknik kriopreservasi untuk penyimpanan semen unggas. Wartazoa. 21:145-152.

Kostaman T, Sopiyana S. 2015. Isolasi gonadal germ cell (GGC) dari perkembangan awal embrio ayam Kampung Unggul Badan Litbang Pertanian (KUB). Dalam: Darodjah S, Setiawan I, Susilawati I, Sulistyati M, Hidayati YA, penyunting. Prosiding Seminar Nasional Peternakan Berkelanjutan 7. Sumedang, 11 November 2015. Sumedang (Indonesia): Universitas Padjadjaran; p. 44-48.

Kostaman T, Yusuf TL, Fahrudin M, Setiadi MA. 2013. Isolasi dan jumlah primordial germ cell sirkulasi (PGC-sirkulasi) pada stadium perkembangan embrio ayam Gaok. JITV. 18:27-33.

van de Lavoir MC, Diamond JH, Leighton PA, Mather-Love C, Heyer BS, Bradshaw R, Kerchner A, Hooi LT, Gessaro TM, Swanberg SE, et al. 2006. Germline transmission of genetically modified primordial germ cells. Nature. 441:766-769.

Liu C, Khazanehdari KA, Baskar V, Saleem S, Kinne J, Wernery U, Chang IK. 2012. Production of chicken progeny (Gallus gallus domesticus) from interspecies germline chimeric duck (Anas domesticus) by primordial germ cell transfer. Biol Reprod. 86:1-8.

Lyall J, Irvine RM, Sherman A, McKinley TJ, Núñez A, Purdie A, Outtrim L, Brown IH, Rolleston-Smith G, Sang H, Tiley L. 2011. Suppression of avian influenza transmission in genetically modified chickens. Science. 331:223-226.

Macdonald J, Taylor L, Sherman A, Kawakami K, Takahashi Y, Sang HM, McGrew MJ. 2012. PNAS plus: efficient genetic modification and germ-line transmission of primordial germ cells using piggyBac and Tol2 transposons. Proc Natl Acad Sci. 109:E1466-E1472.

Meyer DB. 1964. The migration of primordial germ cells in the chick embryo. Dev Biol. 10:154-190.

Moore DT, Purdy PH, Blackburn HD. 2006. A method for cryopreserving chicken primordial germ cells. Poult Sci. 85:1784-1790.

Mozdziak PE, Angerman-Stewart J, Rushton B, Pardue SL, Petitte JN. 2005. Isolation of chicken primordial germ cells using fluorescence-activated cell sorting. Poult Sci. 84:594-600.

Nakajima Y, Minematsu T, Naito M, Tajima A. 2011. A new method for isolating viable gonadal germ cells from 7-day-old chickem embryos. J Poult Sci. 48:106-111.

Nakajima Y, Naito M, Tajima A. 2012. Production of germline chimeras by the transfer of gonadal germ cells (GGCs) recovered from 7-day-old chick embryos by using newly developed PBS [−] method. World Poult Sci J. 68:2012.

Nakajima Y, Tajima A. 2013. Development of a novel method for isolating gonadal germ cells from early chick embryos. J Dev Sustain Agric. 8:75-78.

Nakamura Y, Tasai M, Takeda K, Nirasawa K, Tagami T. 2013. Production of functional gametes from cryopreserved primordial germ cells of the Japanese Quail. J Reprod Dev. 59:580-587.

Pain B, Clark ME, Shen M, Nakazawa H, Sakurai M, Samarut J, Etches RJ. 1996. Long-term in vitro culture and characterisation of avian embryonic stem cells with multiple morphogenetic potentialities. Development. 122:2339-2348.

Park TS, Han JY. 2012. PiggyBac transposition into primordial germ cells is an efficient tool for transgenesis in chickens. Proc Natl Acad Sci USA. 109:9337-9341.

Park TS, Kim MA, Lim JM, Han JY. 2008. Production of quail (Coturnix japonica) germline chimeras derived from in vitro-cultured gonadal primordial germ cells. Mol Reprod Dev. 75:274-281.

Petitte JN, Clark ME, Liu G, Gibbins AM V, Etches RJ. 1990. Production of somatic and germline chimeras in the chicken by transfer of early blastodermal cells. Development. 108:185-189.

Salter DW, Smith EJ, Hughes SH, Wright SE, Fadly AM, Witter RL, Crittenden LB. 1986. Gene insertion into the chicken germline by retroviruses. Poult Sci. 65:1445-1458.

Sawicka D, Brzezinska J, Bednarczyk M. 2011. Cryoconservation of embryonic cells and gametes as a poultry biodiversity preservation method. Folia Biol. 59:1-5.

Sawicka D, Chojnacka-Puchta L, Zielinski M, Plucienniczak G, Plucienniczak A, Bednarczyk M. 2015. Flow cytometric analysis of apoptosis in cryoconserved chicken primordial germ cells. Cell Mol Biol Lett. 20:143-159.

Sekido R, Lovell-Badge R. 2007. Mechanisms of gonadal morphogenesis are not conserved between chick and mouse. Dev Biol. 302:132-142.

Setioko AR. 2008. Konservasi plasma nutfah unggas melalui kriopreservasi primordial germ cells (PGCs). Wartazoa. 8:68-77.

Setioko AR, Tagami T, Tase H, Nakamura Y, Takeda K, Nirasawa K. 2007. Cryopreservation of primordial germ cells (PGCs) from White Leghorn embryos using commercial cryoprotectants. J Poult Sci. 44:73-77.

Squires EL, Keith SL, Graham JK. 2004. Evaluation of alternative cryoprotectants for preserving stallion spermatozoa. Theriogenology. 62:1056-1065.

Tajima A, Barbato GF, Kuwana T, Hammerstedt RH. 2003. Conservation of a genetically selected broiler line (42L) using cryopreserved circulating primordial germ cells (PGCs) isolated by filtration method. J Poult Sci. 40:53-61.

Tajima A, Hayashi H, Kamizumi A, Ogura J, Kuwana T, Chikamune T. 1999. Study on the concentration of circulating primordial germ cells (cPGCs) in early chick embryos. J Exp Zool. 284:759-764.

Tajima A, Minematsu T, Ohara M. 2004. Production of germ-line chimeras by the transfer of cryopreserved gonadal germ cells collected from 7 and 9-day old chick embryos. J Anim Sci. 75:85-88.

Tajima A, Naito M, Yasuda Y, Kuwana T. 1998. Production of germ-line chimeras by transfer of cryopreserved gonadal primordial germ cells (gPGCs) in chicken. J Exp Zool. 280:265-267.

Tsunekawa N, Naito M, Sakai Y, Nishida T, Noce T. 2000. Isolation of chicken vasa homolog gene and tracing the origin of primordial germ cells. Development. 127:2741-2750.

Ukeshima A, Yoshinaga K, Fujimoto T. 1991. Scanning and transmission electron microscopic observations of chick primordial germ cells with special reference to the extravasation in their migration course. J Electron Microsc. 40:124-128.

Vick L, Li Y, Simkiss K. 1993. Transgenic birds from transformed primordial germ cells. Proc R Soc B. 251:179-182.

Yang JH, Kim S. 2012. Establishment of an efficient and stable transgene expression system in chicken primordial germ cell. Bull Korean Chem Soc. 33:1536-1540.

Yasuda Y, Tajima A, Fujimoto T, Kuwana T. 1992. A method to obtain avian germ-line chimaeras using isolated primordial germ cells. J Reprod Fertil. 96:521-528.

Zhu L, van de Lavoir MC, Albanese J, Beenhouwer DO, Cardarelli PM, Cuison S, Deng DF, Deshpande S, Diamond JH, Green L, et al. 2005. Production of human monoclonal antibody in eggs of chimeric chickens. Nat Biotechnol. 23:1159-1169.

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