The Use of Mycotoxin Binder to Control Its Contamination in Feed

Prima Mei Widiyanti, Romsyah Maryam


The climate in Indonesia as a tropical country is very condusive for the growth of mycotoxins producing fungi. Mycotoxins have properties as carcinogenic, mutagenic, teratogenic, estrogenic, neurotoxic, and immunotoxic. Mycotoxins reduce performance, appetite, weight, and immunity. They also cause reproductive disorders and generate the residues in animal products that affect human health. These can be prevented by controlling mycotoxins contamination in agricultural products that used for feed ingredients through good management practices (during planting, harvesting, and storage). Mycotoxins contamination can also be minimized by physical, chemical and biological treatments as well as the application of mycotoxin binders. This review describes the use of mycotoxin binders in animal feed. They are used as feed additives, may be derived from organic, inorganic materials or their combination. Combination of organic and inorganic substances proven to be more effective and efficient in controlling mycotoxin contamination. Therefore, it is recommended to use mycotoxin binders to prevent animal health disorder and to decrease mycotoxin residues in animal products.



Mycotoxins; contamination; feed; mycotoxins binders

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Abbas HK. 2005. Afaltoxin and food safety. London (UK): CRC Press.

Agrina. 2014. Cermat memilih antimikotoksin. Agrina. [Internet]. [disitasi 17 Februari 2016] Tersedia dari: aid=4843.

Allameh A, Safamehr A, Mirhadi SA, Shivazad M, Razzaghi-Abyaneh M, Afshar-Naderi A. 2005. Evaluation of biochemical and production parameters of broiler chicks fed ammonia treated aflatoxin contaminated maize grains. Anim Feed Sci Technol. 122:289-301.

Attia YA, Allakany HF, Abd Al-Hamid AE, Al-Saffar AA, Hassan RA, Mohamed NA. 2013. Capability of different non-nutritive feed additives on improving productive and physiological traits of broiler chicks fed diets with or without aflatoxin during the first 3 weeks of life. J Anim Physiol Anim Nutr (Berl). 97:754-772.

Avantaggiato G, Havenaar R, Visconti A. 2003. Assessing the zearalenone-binding activity of adsorbent materials during passage through a dynamic in vitro gastrointestinal model. Food Chem Toxicol. 41:1283-1290.

Avantaggiato G, Havenaar R, Visconti A. 2004. Evaluation of the intestinal absorption of deoxynivalenol and nivalenol by an in vitro gastrointestinal model, and the binding efficacy of activated carbon and other adsorbent materials. Food Chem Toxicol. 42:817-824.

Avantaggiato G, Havenaar R, Visconti A. 2007. Assessment of the multi-mycotoxin-binding efficacy of a carbon/aluminosilicate-based product in an in vitro gastrointestinal model. J Agric Food Chem. 55:4810-4819.

Bahri S, Maryam R, Widiastuti R. 2005. Cemaran aflatoksin pada bahan pakan dan pakan di beberapa daerah Provinsi Lampung dan Jawa Timur. JITV. 10:236-241.

Bahri S, Maryam R. 2003. Mikotoksin berbahaya dan pengaruhnya terhadap kesehatan hewan dan manusia. Wartazoa. 14:129-142.

Baron S. 1996. Medical microbiology. Texas (US): University of Texas Medical.

Bindhu L V, Jin KT. 2010. Mycotoxin binders-evaluation of efficiency by the in vitro method. J Int Poult Prod. 18.

Boardini JG, Borsato D, Oliveira AS, Ono MA, Zaninelli TH, Hirooka E, Ono E. 2014. In vitro Zaeralenone adsorption by a mixture of organic and inorgnik adsorbents: Aplication of the box behnken approach. World mucotoxin J. 291-299.

Brezonik PL, Arnold WA. 2011. Water chemistry: An introduction to the chemistry of natural and engineered aquatic systems. New York (US): Oxford University Press.

De Mil, Devreese M, Baere SD, Ranst EV, Leckhout M, De Backer, Croubels. 2015. Characterization of 27 Mycotoxin binders & the relation with in vitro zearalenone adsorption at a single concentration. J toxins. 7:21-33.

Deng Y, Liu L, Velazquez ALB, Szczerba, Dixon JB. 2014. Interaction of aflatoxin B1 with smettites: Inter accesibility, banding mechanisms, and size macthing. In: Dixon JB, Velázquez ALB, Deng Y, editors. Aflatoxin control: Safeguarding animal feed with calcium smectite. Wisconsin (US): American Society of Agronomy and Soil Science Society of America. p. 27-43.

Devreese M, Antonissen G, De Backer P, Croubels S. 2014. Efficacy of active carbon towards the absorption of deoxynivalenol in pigs. Toxins (Basel). 6:2998-3004.

Devreese M, De Backer P, Croubels S. 2013. Different methods to counteract mycotoxin production and its impact on animal health. Vlaams Diergeneeskd Tijdschr. 82:181-189.

Dharmaputra OS. 2014. Fungi, mycotoxins and their control in Indonesian food and feedstuff. Bogor (Indonesia): Seameo Biotrop.

Diaz DE. 2008. A review on the use of mycotoxin sequestering agents in agricultural livestock production. Food contaminants. p.125-150.

Edrington TS, Kubena LF, Harvey RB, Rottinghaus GE. 1997. Influence of a superactivated charcoal on the toxic effects of aflatoxin or T-2 toxin in growing broilers. Poult Sci. 76:1205-1211.

EFSA. 2009. Review of Mycotoxin-detoxifying agents used as feed additives: Mode of action, efficacy and feed/food safety. European Food Safety Authority [Internet]. [cited 2014 Feb 4]. Available from:

EFSA. 2011. Scientific opinion on the safety and efficacy of bentonite (dioctabedral mont morillonite) as a feed additive for all species. European Food Safety Authority [Internet]. [cited 2016 Feb 19]. Available from: -output/2007.pdf

EFSA. 2012. Guiddance for the preparation of dossiers for technological additives. European Food Safety Authority [Internet]. [cited 2016 Feb 21]. Available from:

van der Eijk C. 2003. New technologies improve mycotoxin elimination. Feed Mix. 11:8-10.

Fruhauf S, Schwartz H, Ottner F, Krska R, Vekiru E. 2012. Yeast cell based feed additives: Studies on aflatoxin B₁ and zearalenone. Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 29:217-31.

Ghahri H, Habibia R, Fam MA. 2010. Effect of sodium bentonite, mannan oligosaccaharide and humate on performance and serum biochemical parameters during aflatoxicosis in broiler chickens. J Glob Vet. 5:129-134.

Grenier B, Applegate TJ. 2012. Reducing the impact of aflatoxins in livestock and poultry. ANSC [Internet]. [cited 2014 Feb 4]. Available from:

Gratz S, Mykkanen H, El-Nezami H. 2005. Aflatoxin B1 binding by a mixture of Lactobacillus and Propionibacterium: in vitro versus ex vivo. J Food Protect. 68: 2470-2474.

Hartinger D, Moll W. 2011. Fumonisin elimination and prospects for detoxification by enzymatic transformation. World Mycotoxin J. 4:271-283.

Herzallah AM. 2013. Aflatoxin B1 residues in eggs and flesh of laying hes fe aflatoxin B1 contaminated diet. Am J Agric Biol Sci. 8:156-161.

Huwig A, Freimund S, Käppeli O, Dutler H. 2001. Mycotoxin detoxication of animal feed by different adsorbents. Toxicol Lett. 122:179-188.

Jaynes WF, Zartman RE. 2011. Aflatoxin toxicity reduction in feed by enhanced binding to surface-modified clay additives. Toxins (Basel). 3:551-565.

Jouany JP, Yiannikouris, Bertin G. 2005. The chemical bonds betwen mycotoxins and cell wall component of Saccaromyces cerevisiae have been identified. Arch Zootech. 2005:26-50.

Khadem AA, Sharifi SD, Barati M, Borji M. 2012. Evaluation of the effectiveness of yeast, zeolite and active charcoal as aflatoxin absorbents in broiler diets. Glob Vet. 8:426-432.

Khotimah K, Hartatie ES. 2012. Pengembangan formulasi konsentrat sapi perah dengan toksin binder untuk peningkatan kuantitas dan kualitas susu. Malang (Indonesia): Universitas Muhammadyah Malang.

Kolosova A, Stroka J. 2011. Substances for reduction of the contamination of feed by mycotoxins: A review. World Mycotoxin J. 4:225-256.

Kolossova A, Stroka J, Breidbach A, Kroeger K, Ambrosio M, Bouten K, Ulberth F. 2009. Evaluation of the effect mycotoxin binders in animal feed on the analytical performance of standardised methods for the determination of mycotoxin in feed. JRC Eur. 54375:1-46.

Lattanzio VMT, Solfrizzo M, Powers S, Visconti A. 2007. Simultaneous determination of aflatoxins, ochratoxin A and Fusarium toxins in maize by liquid chromatography/tandem mass spectrometry after multitoxin immunoaffinity cleanup. Rapid Commun Mass Spectrom. 21:3253-3261.

Machacek M, Vecerek V, Mas N, Suchy P, Strakova E, Serman V, Herzig I. 2010. Effect of the feed additive clinoptilolite (zeofeed) on nutrition metabolism and production performance of laying hens. J Acta Vet. 79:29-34.

Manafi M, Murthy HNN, Pirany N, Swamy HNN. 2012. Comparative study of saveral mycotoxin binders during aflatoxicosis in body weight, feed consumption, feed efficiency & egg production parameters of broiler breeders. J Global Vet. 8:484-490.

Maryam R, Nehat H, Firmansyah R, Djuariah S, Miharja. 2001. Efektivitas natrium kalsium aluminosilikat hidrat dalam penurunan residu aflatoksin pada daging dan hati ayam broiler. Dalam: Haryanto B, Setiadi B, Sinurat AP, Mathius IW, Situmorang P, Nurhayati, Ashari, Abubakar, Murdiati TB, Hastiono S, et al., penyunting. Prosiding Seminar Nasional Teknologi Peternakan dan Veteriner. Bogor, 17-18 September 2001. Bogor (Indonesia): Puslitbangnak. hlm. 708-715.

Maryam R. 2006. Pengendalian terpadu kontaminasi mikotoksin. Wartazoa. 16:21-30.

Miazzo R, Rosa C a, De Queiroz Carvalho EC, Magnoli C, Chiacchiera SM, Palacio G, Saenz M, Kikot A, Basaldella E, Dalcero A. 2000. Efficacy of synthetic zeolite to reduce the toxicity of aflatoxin in broiler chicks. Poult Sci. 79:1-6.

Niknejad F, Zaini F, Faramarzi M, Amini M, Kordbacheh P, Mahmoudi M, Safara M. 2012. Candida parapsilosis as a potent biocontrol agent against growth and aflatoxin production by Aspergillus species. Iran J Public Health. 41:72-80.

Olvera JEV, Garcia HA, Hernandez AB, Vergara NVP, Moroyoqui FJC. 2012. Evaluation of pathological effects in broilers during fumonisins and clays exposure. J Mycopathologia. 174: 247-254.

Ortatatli M, Oguz H, Hatipoglu F, Karaman M. 2005. Evaluation of pathological changes in broilers during chronic aflatoxin (50 and 100 ppb) and clinoptilolite exposure. J Res Vet Sci. 78:61-68.

Phillips TD, Afriyie-Gyawu E, Williams J, Huebner H, Ankrah N, Ofori-Adjei D, Jolly P, Johnson N, Taylor J, Marroquin-Cardona A, et al. 2008. Reducing human exposure to aflatoxin through the use of clay: A review. Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 25:134-145.

Phillips TD. 1999. Dietary clay in the chemoprevention of aflatoxin-induced disease. Toxicol Sci. 52:118-126.

Rachmawati S. 2001. Efektifitas bahan pengikat mikotoksin (uji in vitro). Dalam: Haryanto B, Setiadi B, Sinurat AP, Mathius IW, Situmorang P, Nurhayati, Ashari, Abubakar, Murdiati TB, Hastiono S, et al., Prosiding Seminar Nasional Teknologi Peternakan dan Veteriner. Bogor, 17-18 September 2001. Bogor (Indonesia): Puslitbangnak. hlm. 924-929.

Rachmawati S. 2005. Aflatoksin dalam pakan di Indonesia: Persyaratan kadar dan pengembangan teknik deteksinya. Wartazoa. 15:26-37.

Rachmawati S. 2007. Peningkatan kemanan produk ternak dari kontaminan aflatoksin menggunakan toxin binder. Dalam: Seminar Nasional Hari Pangan Sedunia XXVII. Bogor (Indonesia): Puslitbangnak. hlm. 212-215.

Rachmawati S. 2010. Efektivitas zeolit komersial sebagai bahan pengikat aflatoksin (uji in vitro). Dalam: Prasetyo LH, Natalia L, Iskandar S, Puastuti W, Herawati T, Nurhayati, Anggraeni A, Damayanti R, Dharmayanti NLI, Estuningsih SE, penyunting. Teknologi Peternakan dan Veteriner Ramah Lingkungan dalam Mendukung Program Swasembada Daging dan Peningkatan Ketahanan Pangan. Prosiding Seminar Nasional Teknologi Peternakan dan Veteriner. Bogor, 3-4 Agustus 2010. Bogor (Indonesia): Puslitbangnak. hlm. 837-842.

Saeger SD. 2011. Determining mycotoxins and mycotoxigenic fungi in food and feed. Cambridge (UK): Woodhead Publishing.

Saepulloh M, Bahri S, Rahmawati S, Dharmayanti NLP. 2011. Pengaruh toxin binder & aflatoxin B1 terhadap respon tanggap kebal Newcastle disease pada ayam pedaging. Dalam: Prasetyo LH, Damayanti R, Iskandar S, Herawati T, Priyanto D, Puastuti P, Anggraeni A, Tarigan S, Wardhana AH, Dharmayanti NLPI, penyunting. Teknologi Peternakan dan Veteriner untuk Peningkatan Produksi dan Antisipatif terhadap Dampak Perubahan Iklim. Prosiding Seminar Nasional Teknologi Peternakan dan Veteriner. Bogor, 7-8 Juni 2011. Bogor (Indonesia): Puslitbangnak. hlm. 753-764.

Schatzmayr G, Zehner F, Taubel M, Schatzmayr D, Klimitsch A, Loibner AP, Binder EM. 2006. Microbiologicals for deactivating mycotoxins. Mol Nutr Food Res. 50:543-551.

Smith T, Macdonald E, Haladi S. 2001. Dietary prevention of mycotoxicosis. Alltech 17th Annu Feed Ind Symp. 9:30-32.

Solfrizzo M, Visconti A, Avantaggiato G, Torres A, Chulze S. 2001a. In vitro and in vivo studies to asses the effectivenes of cholestyramine as a binding agent to fumonisins. Mycoploethologia. 151:147-153.

Solfrizzo M, Carratu MR, Avantaggiato G, Galvano F, Pietri A, Visconti A. 2001b. Ineffectiveness of activated carbon in reducing the alteration of sphingolipid metabolism in rats exposed to fumonisin-contaminated diets. Food Chem Toxicol. 39:507-511.

Sumantri I, Murti TW, Boehm J, Agus A. 2012. Kapasitas dan stabilitas pengikatan beberapa adsorben aflatoksin alami di dalam rumen in vitro. Bul Peternakan. 36:156-161.

Topcu A, Bulat T, Wishah R, Boyaci IH. 2010. Detoxification of aflatoxin B1 and patulin by Enterococcus faecium strains. Int J Food Microbiol. 139:202-205.

Vekiru E, Fruhauf S, Rodrigues J, Otlner F, Krska R, Schatzmayr G, Ledoux DR, Rottinghaus GE. 2015. In vitro binding assesment and in vivo efficacy of saveral adsorbents againts aflatoxin B. World Mycotoxin J. 8:477-488.

Widiastuti R, Maryam R, Bahri S. 2006. Aflatoksin M1 pada susu sapi segar Pangalengan dan Bogor, Jawa Barat. In: Mathius IW, Sendow I, Nurhayati, Murdiati TB, Thalib A, Beriajaya, Prasetyo LH, Darmono, Wina E, penyunting. Cakrawala Baru Iptek Menunjang Revitalisasi Peternakan. Prosiding Seminar Nasional Teknologi Peternakan dan Veteriner. Bogor, 5-6 September 2006. Bogor (Indonesia): Puslitbangnak. hlm. 239–243.

Widiastuti R. 2014. Residu aflatoksin dan metabolitnya pada berbagai produk pangan asal hewan dan pencegahannya. Wartazoa. 24:179-190.

Yiannikouris A, François J, Poughon L, Dussap CG, Bertin G, Jeminet G, Jouany JP. 2004. Adsorption of Zearalenone by beta-D-glucans in the Saccharomyces cerevisiae cell wall. J Food Prot. 67:1195-1200.

Zain ME. 2010. Impact of mycotoxins on humans and animals. J Saudi Chem Soc. 15:129-144.


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