Aktivitas Daun Bambu sebagai Anthelmintik cacing Haemonchus contortus pada Kambing Bligon secara in vitro

B P Widiarso, W Nurcahyo, F Ekawasti

Abstract

Haemonchosis disebabkan oleh cacing nematoda Haemonchus contortus,dengan karakteristik menghisap darah, hidup di abomasum ruminansia dan dapat menyebabkan kerugian ekonomi yang tinggi di seluruh dunia. Parasit ini dapat menyebabkan anemia, edema dan jika tidak ditangani serius dapat menimbulkan kematian. Penanganan dan pengendalian menggunakan anthelmintika kimia yang berspektrum luas dapat menjadi ancaman serius kesehatan konsumen akibat timbulnya resistensi dan residu jika digunakan secara terus menerus dalam waktu yang lama.. Kandungan tannin dalam daun bambu berpotensi sebagai agen anthelmintik. Penelitian ini bertujuan untuk melihat potensi anthelmintic daun bambu terhadap tingkat mortalitas cacing dewasa secara in vitro dan keberadaan profil protein dalam H. contortus. Cacing H. contortus diperoleh dari abomasum kambing bligon terinfeksi H. contortus yang dipotong di RPH Sleman. H. contortus direndam pada ekstrak larutan daun bambu 0,1% dan 1%. Isolasi protein H. Contortus untuk melihat profil protein cacing dari masing-masing perlakuan tersebut. Protein yang dianalisis melalui SDS-PAGE menunjukkan hanya 6 pita protein dominan dengan berat molekul 9 - 125 kDa. Infusa daun bambu memiliki potensi sebagai anthelmetika karena mampu merusak beberapa protein Haemonchus contortus.

 Keywords

Daun bambu; anthelmintik; protein; Hemonchus contortus; kambing

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DOI: http://dx.doi.org/10.14334/Pros.Semnas.TPV-2020-p.872-882

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