Patologi Komparatif Itik dan Ayam yang Diinfeksi Buatan dengan Virus HPAI H5N1-Clade 2.3.2

R Damayanti, R Indriani, H Nuradji

Abstract

Wabah virus Avian Influenza yang sangat patogen H5N1 (HPAI) masih terdapat di Indonesia.Virus HPAI H5N1, clade 2.3.2 dilaporkan menyebabkan kematian pada itik dan ayam. Tujuan dari penelitian ini adalah untuk membandingkan perubahan patologi yang diamati pada itik dan ayam yang diinfeksi HPAI H5N1 clade 2.3.2. Sembilan ekor ayam sehat dan sembilan itik secara eksperimental diinfeksi virus HPAI H5N1 clade 2.3.2. (A / bebek / Sukoharjo / BBVW-1428-9 / 2012). Tanda-tanda klinis dan mortalitas dicatat, sejumlah organ dikoleksi untuk diproses sebagai preparat histopatologi. Pewarnaan imunohistokimia dilakukan untuk mendeteksi distribusi antigen dalam jaringan yang terserang dan untuk mengetahui patogenesis penyakitnya. Hasil penelitian menunjukkan bahwa baik itik maupun ayam rentan terhadap infeksi HPAI H5N1 clade 2.3.2. yang ditunjukkan oleh kematian yang ditemukan pada unggas ini. Lesi patologis bervariasi derajat keparahannya dan ayam dan itik masing-masing mempunyai predileksi terhadap organ organ tertentu yang berkorelasi positif dengan level antigen yang dideteksi pada organ terkait. Secara histopatologis lesi yang berupa ensefalitis, sinusitis, trakheitis, pneumonia, dermatitis pada ayam derajat keparahannya lebih ringan daripada lesi serupa pada itik. Sedangkan konjungtivitis dan pankreatitis hanya ditemukan pada itik yang diinfeksi H5N1 clade 2.3. Itik mempunyai predileksi lebih besar terhadap organ nasal, orbital (mata), folikel bulu dan pankreas, sedangkan pada ayam predileksinya ada pada organ proventrikulus, hati, limpa, usus dan bursa fabrisius.

 Keywords

HPAI H5N1clade 2.3.2; duck; chicken; histopatologi; imunohistokimia

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DOI: http://dx.doi.org/10.14334/prossemnastpv.v20i20.2648

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