Keragaman Gen IGF1 Exon 4 pada Kambing Gembrong, Samosir dan Kosta di Stasiun Penelitian Kambing Potong Sumatera Utara

F Mahmilia, N Alwiyah, A Destomo

Abstract

Pelestarian dan pemanfaatan sumber daya genetik merupakan hal penting dalam rangka meningkatkan potensi ternak local di Indonesia khususnya kambing. Salah satu upaya peningkatan potensi adalah dengan mengarahkannya ke program pemuliaan yaitu seleksi terhadap marka genetik yang berperngaruh terhadap pertumbuhannya salah ssatunya adalah gen IGF1 exon 4. Materi yang digunakan adalah kambing Samosir (5 ekor), Kosta (14 ekor) dan Gembrong (8 ekor). Primer forward yang digunakan dalam penelitian ini adalah 5’- CACAGCGTATTATCCCAC-3’ dan primer reverse 5’- GACACTATGAGCCAGAAG -3’. Ditemukan satu SNP saja yaitu c.267G>C. SNP tersebut bersifat polimorfik pada kambing Gembrong, Samosir dan Kosta. Genotipe CC memiliki frekuensi yang lebih tinggi pada ketiga kambing. Heterozigositas kambing Gembrong dan Kosta berada dalam keseimbangan genotipe sedangkan kambing Samosir tidak berada dalam keseimbangan dan memiliki nilai HW yang tidak nyata.

 Keywords

Kambing Samosir; kosta; Gembrong; Gene IGF1 exon 4; keragaman

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DOI: http://dx.doi.org/10.14334/prossemnastpv.v20i20.2613

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