Eksplorasi Genetik dari Lokus GH|MspI Ekson 3 dan GHRH|HaeIII Intron 2 pada Kerbau Rawa di Stasiun Bibit dan Peternak Rakyat

A Anggraeni, C Thalib, W T Novitasari

Abstract

Perbaikan produktivitas ternak dapat dilakukan melalui seleksi secara molekular pada gen-gen pengontrol sifat pertumbuhan. Keragaman genetik dari famili gen pertumbuhan yaitu gen GH dan GHRH dipelajari pada kerbau rawa dari stasiun pembibitan BPTU Siborong-borong (ST) (68 betina) di Kabupaten Tapanuli Utara, Provinsi Sumatera Utara dan peternak rakyat (PR) 37 betina, 4 jantan) di Kabupaten Pandeglang, Provinsi Banten. Varian genetik dari lokus GH|MspI ekson 3 dan GHRH|HaeIII intron 2 digenotyping dengan metode Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP). Data dianalisis terhadap frekuensi alel dan genotipe, keseimbangan Hardy-Weinberg, nilai heterozigositas, dan nilai PIC. Hasil genotyping dari lokus GH|MspI ekson 3 dan GHRH|HaeIII inton 2 menghasilkan hanya alel B (100%) pada kerbau rawa dari ST, sebaliknya pada PR hanya alel A (100 %). Keseimbangan Hardy-Weinberg tidak dapat dianalisis untuk kedua lokus. Nilai heterozigositas pengamatan (Ho) dan harapan (He) serta nilai PIC untuk setiap lokus adalah sebesar 0.00. Disimpulkan bahwa lokus GH MspI ekson 3 dan GHRH HaeIII intron 2 pada kerbau rawa lokal pengamatan bersifat monomorfik.

 Keywords

Famili gen pertumbuhan; PCR-RFLP; kerbau rawa

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DOI: http://dx.doi.org/10.14334/Pros.Semnas.TPV-2020-p.41-51

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