Kontrol Positif Sintetik untuk Deteksi Penyakit Mulut dan Kuku dengan Reverse-Transcriptase Polymerase Chain Reaction

H Nuradji, A Wiyono, A Rochmah

Abstract


Penyakit Mulut dan Kuku merupakan penyakit hewan menular strategis dan masih eksotik di Indonesia. Untuk mengantisipasi penyakit ini diperlukan deteksi penyakit yang cepat dan akurat. Uji RT-PCR merupakan salah satu uji yang direkomendasikan untuk deteksi penyakit ini. Namun demikian, uji ini memerlukan kontrol positif yang dapat digunakan dalam setiap pengujian. Tujuan dari penelitian ini adalah mengembangkan sintetik kontrol positif untuk deteksi penyakit PMK dengan uji RT-PCR. Kontrol positif didesign berdasarkan primer yang direkomendasikan Organisasi Kesehatan Hewan Dunia (OIE). Kontrol positif kemudian diuji dengan uji RT-PCR dan dibandingkan dengan kontrol positif yang diperoleh dari antigen inaktif yang digunakan dalam uji ELISA. Hasil menunjukkan bahwa sintetik kontrol positif yang dikembangkan memberikan hasil yang yang lebih baik dibandingkan dengan kontrol positif yang diekstraksi dari antigen inaktif. Hal ini menunjukkan bahwa kontrol positif sintetik ini dapat digunakan dalam pengujian RT-PCR untuk mendeteksi PMK di Indonesia.

Keywords


Kontrol Positif Sintetik; PMK; RT-PCR

References


Ahmed HA, Salem SAH, Habashi AR, Arafa AA, Aggour MGA, Salem GH, Gaber AS, Selem O, Abdelkader SH, Knowles NJ. 2012. Emergence of foot and mouth disease virus SAT 2 in Egypt during 2012. Transbound Emerg Dis. 59:476-481.

Caasi DRJ, Arif M, Payton M, Melcher U, Winder L, Ochoa-Corona FM. 2013. A multi-target, non-infectious and clonable artificial positive control for routine PCR-based assays. J Microbiol Methods. 95:229-234.

Chen H, Peng Y, Zhang Y, Liu X. 2013. Detection of foot and mouth disease Serotype O by ELISA using a monoclonal antibody. Monoclon Antib Immunodiagn Immunother. 32:47-49.

Grubman MJ, Baxt B. 2004. Foot and mouth disease. Clin Microbiol Rev [Internet]. 17:465-93. available at https://www.ncbi.nlm.nih.gov/pubmed/15084510.

Habib M, Shah MS, Muzammil HM, Manzoor S, Khan RSA, Munir R, Rajput ZI, Farooq U. 2014. Investigations of foot and mouth disease outbreaks in Faisalabad District of Punjab, Pakistan during the Year 2013. Life. 12:165-169.

Jamal SM, Belsham GJ. 2013. Foot and mouth disease: past, present and future. Vet Res. 44:1-14.

Kandeil A, El-Shesheny R, Kayali G, Moatasim Y, Bagato O, Darwish M, Gaffar A, Younes A, Farag T, Kutkat MA. 2013. Characterization of the recent outbreak of foot-and-mouth disease virus serotype SAT2 in Egypt. Arch Virol. 158:619-627.

Kementerian Pertanian. 2013. Keputusan Menteri Pertanian tentang penetapan penyakit hewan menular strategis. Jakarta (Indonesia): Kementerian Pertanian.

Lung O, Fisher M, Beeston A, Hughes KB, Clavijo A, Goolia M, Pasick J, Mauro W, Deregt D. 2011. Multiplex RT-PCR detection and microarray typing of vesicular disease viruses. J Virol Methods. 175:236-245.

Ma L, Zhang J, Chen H, Zhou J, Ding Y, Liu Y. 2011. An overview on ELISA techniques for FMD. Virol J. 8:419.

Nandi SP, Rahman MZ, Momtaz S, Sultana M, Hossain MA. 2015. Emergence and distribution of foot and mouth disease virus serotype A and O in Bangladesh. Transbound Emerg Dis. 62:328-331.

OIE. 2012. Foot and mouth disease. Terr Anim Heal Code.:1-26. [Internet]. Available from: http://www.oie.int/en/international-standard-setting/terrestrial-code/access-online/?htmfile=sommaire.htm.

Reid SM, Ferris NP, Hutchings GH, Samuel AR, Knowles NJ. 2000. Primary diagnosis of foot-and-mouth disease by reverse transcription polymerase chain reaction. J Virol Methods. 89:167-176.

Reid SM, Ferris NP, Hutchings GH, Zhang Z, Belsham GJ, Alexandersen S. 2002. Detection of all seven serotypes of foot and mouth disease virus by real-time, fluorogenic reverse transcription polymerase chain reaction assay. J Virol Methods. 105:67-80.

Reid SM, Hutchings GH, Ferris NP, De Clercq K. 1999. Diagnosis of foot and mouth disease by RT-PCR: evaluation of primers for serotypic characterisation of viral RNA in clinical samples. J Virol Methods. 83:113-123.

Smith G, Smith I, Harrower B, Warrilow D, Bletchly C. 2006. A simple method for preparing synthetic controls for conventional and real-time PCR for the identification of endemic and exotic disease agents. J Virol Methods. 135:229-234.

Soehadji, Setyaningsih H. 1994. The experiences of Indonesia in the control and eradication of foot-and-mouth disease. In: Copland JW, Gleeson LJ, Chamnanpool C, editors. Diagnosis Epidemiol Foot and Mouth Dis Southeast Asia. Proceedings of an International Workshop, Lampang, Thailand, 6-9 September 1993. Canberra (Australia): ACIAR 51:64-69.

Wernery U, Kinne J. 2012. Foot and mouth disease and similar virus infections in camelids: a review. Rev Sci Tech. 31:907-918.




DOI: http://dx.doi.org/10.14334/Pros.Semnas.TPV-2017-p.185-191

Refbacks

  • There are currently no refbacks.



PUSAT PENELITIAN DAN PENGEMBANGAN PETERNAKAN
Jl. Raya Pajajaran Kav E59 Bogor 16151 Jawa Barat, Indonesia
Telepon: (+62) 251 8322185, (+62) 251 8328383 e-Mail: medpub329@gmail.com
Website: http://peternakan.litbang.pertanian.go.id