In Vitro Digestibility and Rumen Fermentation of Grass or Rice Straw Basal Diet With or Without Complete Rumen Modifier Supplementation

Dwi Yulistiani, Wisri Puastuti, Yeni Widiawati

Abstract


An in vitro study was conducted to evaluate the supplementation of complete rumen modifier (CRM) to elephant grass or rice straw basal diet. CRM is feed additive consisting of a mixture of defaunator, methanogenesis inhibitor and bacterial growth factors. The diet was formulated in iso energetic and iso protein contained CP 16% and ME 2.3 MJ/kg. The diet was fermented for 48 hours under anaerobic condition at 39 °C. Experiment was conducted in a completely randomized design in 2×2 factorial arrangement using 2 levels of type of basal diet (Napier grass and rice straw) and 2 levels of CRM supplementation (0 and 2%). The parameters recorded were apparent digestibility of grass substrate, gas production, methane production and bacterial and protozoal counts. Total gas and methane produced during incubation was recorded at 2, 4, 8, 12, 16, 24, 36 and 48 hours. Results from the study show that DM and OM digestibility, bacterial and protozoal population were significantly affected by the interaction between CRM supplementation and type of basal diet. CRM supplementation in rice straw basal diet increased DM and OM by 27% and 23,48% respectively, but it did not increase in grass basal diet. Bacterial population was increased by CRM supplementation in grass basal diet, in contrast, in rice straw basal diet, CRM supplementation decreased bacterial population. Whereas protozoal population was decreased both in grass and rice straw basal diet, the decreased was about 63.26% and 64% respectively for grass and rice straw basal diet. Methane production tended (P<0.07) to decrease by CRM supplementation in rice straw basal diet. From this study, it can be concluded that CRM supplementation was able to improve the fermentability of rice straw basal diet and tended to decrease proportion of methane production. CRM supplementation did not have any effect on grass basal diet.

Keywords


Rumen Modifier; Methane; Rumen Fermentation; Supplementation

References


Bharathidhasan A, Viswanathan K, Balakrishnan V, Valli C, Ramesh S, Senthilkumar SMA. 2013. Effects of purified saponin on rumen methanogenesis and rumen fermentation characteristics studied using in vitro gas production technique. Inter J Vet Sci. 2:44-49. www.ijvets.com.

BPS. 2015. Statistik Indonesia 2015. Jakarta (Indonesia): Badan Pusat Stastistik.

FAO. 2013. Mitigation of greenhouse gas emissions in livestock production. In: Gerber PJ, Henderson B, Makkar HPS, editors. Animal Production and Health. Rome (Italy): FAO.

Firkin JL. 1996. Maximizing microbial protein synthesis in the rumen. J Nutr. 126:1347-1354.

Fonty G, Morvan B. 1995. Ruminal methanogenesis and its alternatives. Satellite Symposium of IVth International Symposium on the Nutrition of Herbivores. Clermont-Ferrand, France, September 16-17, 1995. Clermont-Ferrand (France): INRA. p. 34-40.

Manasri N, Wanapat M, Navanukraw. C. 2012. Improving rumen fermentation and feed digestibility in cattle by mangosteen peel and garlic pellet supplementation. Livest Sci. 148:291-295.

Mao HL, Wang JK, Zhou YY, Liu JX. 2010. Effects of addition of tea saponins and soybean oil on methane production, fermentation and microbial population in the rumen of growing lambs. Livest Sci. 129:56-62.

Menke KH, Steingass H. 1988. Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid. Anim Res Dev. 28:7-55.

Ngamsaeng A, Wanapat M, Khampa S. 2006. Effects of Mangosteen peel (Garcinia mangostana) supplementation onrumen ecology, microbial protein synthesis, digestibility andvoluntary feed intake in cattle. Pak J Nutr. 5:445-52.

Norrapoke T, Wanapat M, Foiklang S. 2014. Influence of tropical plant sources containing plant secondary compound on rumen fermentation using in vitro gas fermentation technique. Indian J Anim Sci. 84:1004-1010.

Ogimoto K, Imai S. 1981. Atlas of Rumen Microbiology. Tokyo (Japan): Japan Scientific Societies Press.

Orskov ER, McDonald I. 1979. The estimation ofprotein degradability in the rumen from incubationmeasurements weighted according rate of passage. J Agric Sci. 92:499-503.

Kumar PA. 2012. Enteric methane mitigation technologies for ruminantlivestock: a synthesis of current research and future directions. Environ Monit Assess. 184:1929-1952.

SAS. 2002. SAS/STAT User’s Guide (Release 9). Carry (USA): SAS Inst, Inc.

Sejian V, Shekhawat I, Ujor V, Ezeji T, Lakritz J, Lal R. 2012. Global climate change: enteric methane reduction strategies in livestock. In: Sejian V, et al., eds. Environmental Stress and Amelioration in Livestock Production. Heidelberg (Germany): Springer-Verlag Berlin. Available from: Veerasamy Sejian.http://www.researchgate.com/. Retrieved on: 08 October 2015.

Stewart CS. 1999. Microbial interaction in the rumen and their potential impact on the survival of Escherichia coli 0157. In: Bell CR, Brylinsky M, Johnson-Green P, editors. The Rumen Microbial Ecosystem. Proceedings of the 8th International Symposium on Microbial Ecology. Ilalifax (Canada): Atlantic Canada Society for Microbial Ecology.

Thalib A. 2004. UJi efektivitas saponin buah lerak Sapindus rarak sebagai inhibitor metanogenesis secara in vitro pada system pencernaan rumen. JITV. 9:164-171.

Thalib A, Widyawati Y, Haryanto B. 2010. Penggunaan Complete Rumen Modifier (CRM) pada ternak domba yang diberi hijauan pakan berserat tinggi. JITV 15:97-104.

Thalib A. 2012. Strategi mitigasi metana enterik dalam meningkatkan produktivitas ternak ruminansia. Dalam: Tiesnamurti B, Nurhayati, Herawati T, Widiawati Y, penyunting. Potensi bahan pakan lokal untuk menurunkan gas metana ternak ruminansia. IAARD Press. p. 262-290.

Tjandraatmadja M. 1981. Anaerobic digestion of fibrous materials. [Thesis]. Agricultural Science, University of Melbourne. Melbourne (Australia): University of Melbourne.

Wina E, Muetzel S, Hoffmann E, Makkar HPS, Becker K. 2005. Saponins containing methanol extract of Sapindus rarak affect microbial fermentation, microbial activity and microbial community structure in vitro. Anim Feed Sci Technol. 121:159-174.

Yulistiani D, Thalib A, Haryanto B, Puastuti W. 2012. The total gas and methane productions of grass incubated in vitro using rumen liquor from sheep adapted to complete rumen modifier supplement. In: Koonawootrittriron K, Suwanasapee T, Jaichansukkit T, Jattawa D, Boonyanuwat K, Skunmun P, editors. Proceedings of the 15th AAAP Animal Science Congress 26-30 November 2012, Thammasat University, Rangsit Campus, Thailand. Bangkok (Thailand): AHAT. p. 3070-3073.


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DOI: 10.14334/Proc.Intsem.LPVT-2016-p.310-317

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