Formulation of Crude Enzymes from Bacillus sp. MTS for Cleaning Solution
Edible bird’s nest (EBN) as a kind of functional food has high economic value depending on the quality such as color and hygiene. The purpose of this research was to find the optimum condition for application of keratinolytic enzymes of Bacillus sp. MTS in cleaning EBN. Activating agents for both enzymes were cation divalents, EDTA, reducing agents, organic solutions, and antibacterial agents. Additive compounds that able to increase keratinase activity were used to make cleaning solution and they were tested on EBN and human hair. Alcoholic solutions (25% ethanol, 25% methanol, 25% glycerol), and some divalent metallic ions (Ca2+, Mg2+, Mn2+, Zn2+) were able to increase keratinase while disulfide reductase was solely activated by 0.05 mM EDTA. The activity of both enzymes was inhibited by NaCl and Na-azide. The activity of keratinase of Bacillus sp. MTS in cleaning solution formulated in this research was 2-3 fold as much as control (crude extract) in human hair substrates. Glycerol and cations divalent increasing 2-3 fold keratinase activity in cleaning solution. The solution was successfully applied to cleaning EBN with weight loss approximately 2.3-2.5%.
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