Identification and Antibiotic Resistance of Streptococcus agalactiae from Red Hybrid Tilapia (Oreochromis niloticus) in Local Wet Markets

Ain MZ Auzureen, R H Hamdan, M H Jamaludin

Abstract


The existence of ethiology disease like Streptococcus agalactiae could be major problems in aquaculture field. In this study, investigations of red tilapia sold around local wet market were recorded. To detect the S. agalactiae in wet markets from disease potential and measure the antibiotic susceptibility test. Selected organs such as eyes, gills, heart and intestine were inoculated onto blood agar and incubated at 37°C for 24 h. Morphology of S. agalactiae were Gram-positive, translucent pin point colony, and has oxidase and catalase negative. Eight isolates of S. agalactiae were confirmed using PCR. The isolates samples were susceptible to imipenem (10 µg), gentamicin (10 µg), ciprofloxacin (5 µg) and nalidixic acid (30 µg). In contrast, they were resistance to ampicillin (10 µg), amoxicillin-clavulanic (20/10 µg), erythromycin (5 µg), neomycin (30 µg), norflaxacin (10 µg), streptomycin (10 µg) and trimethoprim-sulphamethoxazole (1.25/23.5 µg). Furthermore, the occurrence of resistant isolates is a matter of public health concern.

Keywords


MAR Index; Local Wet Market; Susceptible Test

References


Aisyhah M, Amal M, Zamri-Saad M, Siti-Zahrah A, Shaqinah N. 2015. Streptococcus agalactiae isolates from cultured fishes in Malaysia manifesting low resistance pattern towards selected antibiotics. J Fish Dis. 38:1093-1098.

Ali A, Hassan D, Saleha AA, Siti-Khairani B, Milud A. 2010. Streptococcus agalactiae the etiological agent of mass mortality in farmed red tilapia (Oreochromis sp). J Anim Vet Adv. 9:2640-2646.

Amal MNA, Zamri-Saad M. 2011. Streptococcosis in tilapia (Oreochromis niloticus): A review. Pertanika J Trop Agric Sci. 34:195-206.

Amal MNA, Kutty BM, Abdullah SZ. 2008. Streptococcosis in red hybrid tilapia (Oreochromis niloticus) commercial farms in Malaysia. Aquac Res. 40:630-632.

Budiati T, Rusul G, Wan-Abdullah WN, Arip YM, Ahmad R, Thong KL. 2013. Prevalence, antibiotic resistance and plasmid profiling of Salmonella in catfish (Clarias gariepinus) and tilapia (Tilapia mossambica) obtained from wet markets and ponds in Malaysia. Aquaculture. 372:127-132.

Castro-Escarpulli G, Figueras M, Aguilera-Arreola G, Soler L, Fernandez-Rendoz E, Aparicio GO, Guarro J, Chacon MR. 2003. Characterisation of Aeromonas spp. isolated from frozen fish intended for human consumption in Mexico. Int J Food Microbiol. 84:41-49.

Evans JJ, Kleusius PH, Gilbert PM, Shoemaker CA, Al-Sarawi MA, Landsberg J, Duremdez R. Al-Marzouki A, Al-Zenki S. 2002. Characterization of β‐haemolytic group B Streptococcus agalactiae in cultured seabream, Sparus auratus L and wild mullet, Liza klunzingeri (day), in Kuwait. J Fish Dis. 25:505-513.

Najiah M, Aqilah N, Lee K, Khairulbariyyah Z, Mithun S, Chowdhury AJK, Shaharom-Harrison F, Nadirah M. 2012. Massive mortality associated with Streptococcus agalactiae infection in cage-cultured red hybrid tilapia Oreochromis niloticus in Como River, Kenyir Lake, Malaysia. J Biol Sci. 12:438-442.

Parker R. 2011. Aquacultue science: Cengage Learning.

Sarter S, Nguyen HNK, Hung LT, Lazard J, Montet D. 2007. Antibiotic resistance in Gram-negative bacteria isolated from farmed catfish. Food Control. 18:1391-1396.

Vivekanandhan G, Savithamani K, Hatha A, Lakshmanaperumalsamy P. 2002. Antibiotic resistance of Aeromonas hydrophila isolated from marketed fish and prawn of South India. Int J Food Microbiol. 76:165-168.

Zamri-Saad M, Amal M, Siti-Zahrah A, Zulkafli A. 2014. Control and prevention of streptococcosis in cultured tilapia in Malaysia: A review. Pertanika J Trop Agric Sci. 37:389-340.


Full Text: PDF

DOI: 10.14334/Proc.Intsem.LPVT-2016-p.561-566

Refbacks

  • There are currently no refbacks.