Application of Dried Blood Sample on Whatman Filter Paper for Detection of Trypanosoma evansi from Cattle in Central Kalimantan by Internal Trascriber Spacer-1 Polymerase Chain Reaction

Dyah Haryuningtyas Sawitri, April H Wardhana, Dias A Dewi, Fitrine Ekawasti, E Widjaja

Abstract


Trypanosoma evansi is a hemoflagella parasite that infects cattle as agents of Surra. The disease causes great economic losses due to decrease in production and death. Rapid and accurate method for early detection is necessary so that the appropriate control can be implemented. In recent years, PCR has been widely applied for detection of Trypanosoma with higher sensitivity. Trypanosoma-species-specific-DNA-sequences multi copy can be used as a target parasite identification. Internal transcribed spacer regions 1 (ITS-1) is a useful marker for descriminating trypanosomes species. The aims of this study was to detect T. evansi in cattle from Central Kalimantan using ITS-1 PCR. A total of 44 bovine blood samples were colleted using Whatman filter paper from Lamandau District of Central Kalimantan Province. PCR was carried out using ITS-1 primers on DNA templete that was isolated from dried blood. The results showed that 27 out of 44 samples (61.4%) were positive for T. evansi. Lamandau District was suspected to be endemic area of Surra and therefore requires a special attention.

Keywords


Trypanosoma evansi; Internal Transcriber Spacer-1; PCR

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DOI: 10.14334/Proc.Intsem.LPVT-2016-p.191-197

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