Antibiotics Resistance of Vibrio spp. Isolated from Diseased Seabass and Tilapia in Cage Culture

Ruhil Hayati Hamdan, T L Peng, B L Ong, M Y S Suhana, N H Hamid, M N F Afifah, M S Raina

Abstract


Vibriosis has become one of the most important bacterial diseases in marine cultured organisms in recent years. This study was focusing on isolation and identification of Vibrio spp. isolated from diseased seabass (Lates calcarifer), tilapia (Oreochromis niloticus) and seawater from Sri Tujuh lagoon in East Coast of Malaysia; also determination of antibiotic resistance patterns among Vibrio spp. Vibrio species isolated from diseased seabass in Pantai Sri Tujuh, Tumpat, Kelantan were screened for their antibiotic sensitivity patterns by Kirby-Bauer method. A total of 47 isolates belonging to three different species were identified which are V. parahaemolyticus, V. vulnificus, and V. alginolyticus. In this study, high incidence of erythromycin, chloramphenicol and sulfamethoxazole resistance was observed among the Vibrio isolates, whereas all isolates were susceptible to oxytetracycline. Vibrio isolates were 96% resistant to one or more different classes of antibiotic, and 17 different resistance patterns were identified. The MAR index of 0.4 indicating the Vibrio spp. in these farmed fish might have been indiscriminately and continuously exposed to those antibiotics during culturing stages of the fish. This study showed that multidrug-resistant Vibrio spp. were common in diseased seabass and tilapia cultured at Sri Tujuh lagoon. These essential findings suggested involvement of seafood in transmission of these pathogen to human. In addition, oxytetracycline can be used as a treatment to combat vibriosis in diseased seabass and tilapia.

Keywords


Vibrio spp.; Lates calcarifer; Oreochromis niloticus; Antibiotic Resistance

References


Abdel-Aziz M, Eissa AE, Hanna M, Okada MA. 2013. Identifying some pathogenic Vibrio or Photobacterium species during mass mortalities of cultured Gilthead seabream (Sparus aurata) and European seabass (Dicentrarchus labrax) from some Egyptian coastal Provinces. Int J Vet Sci Med. 1:87-95.

Baker-Austin C, McArthur JV, Lindell AH, Wright MS, Tuckfield RC, Gooch J, Warner L, Oliver J, Stepanauskas R. 2009. Multi-site analysis reveals widespread antibiotic resistance in the marine pathogen Vibrio vulnificus. Microbial Ecol. 57:151-159.

Biosca EG, Oliver JD, Amaro C. 1996. Phenotypic characterization of Vibrio vulnificus biotype 2, a lipopolysaccharide-based homogeneous o serogroup within Vibrio vulnificus. Appl Env Microb. 62:918-927.

Bondad-Reantaso MG, Subasinghe RP, Arthur JR, Ogawa K, Chinabut S, Adlard R, Tan Z, Shariff M. 2005. Disease and health management in Asian aquaculture. Vet Parasitol. 32:249-272.

CLSI. 2015. Performance standards for antimicrobial susceptibility testing; M100-S25. Wayne (USA): Clinical and Laboratory Standards Institute.

Das SK, Rahman A, Chisti MJ, Ahmed S, Malek MA, Salam MA, Bardhan PK, Faruque ASG. 2014. Changing patient population in Dhaka Hospital and Matlab Hospital of ICDDR, b. Trop Med Int Health. 19:240-243.

Hoa PTP, Nonaka L, Viet PH, Suzuki S. 2008. Detection of the sul1, sul2, and sul3 genes in sulfonamideresistant bacteria from wastewater and shrimp ponds of North Vietnam. Sci Total Environ. 405:377-384.

Hoa PHP, Managaki S, Nakada N, Takada H, Shimizu A, Anh DH, Viet PH, Suzuki S. 2011. Antibiotic contamination and antibiotic-resistant bacteria in animal farms, city canal and aquaculture environments of North Vietnam. Sci Total Environ. 409: 2894-2901.

Kim MS, Cho JY, Choi HS. 2014. Identification of Vibrio harveyi, Vibrio ichthyoenteri, and Photobacterium damselae isolated from olive flounder Paralichthys olivaceus in Korea by multiplex PCR developed using the rpoB gene. Fish Sci. 80:333-339

Krumperman PH. 1983. Multiple antibiotic resistance indexing of Escherichia coli to identify high-risk sources of fecal contamination of foods. Appl Environ Microbiol. 46:165-170.

Laganà P, Caruso G, Minutoli E, Zaccone R, Delia S. 2011. Susceptibility to antibiotics of Vibrio spp. and Photobacterium damsela spp. piscicida strains isolated from Italian aquaculture farms. New Microbiol. 34:53-63.

Lesley MB, Velnetti L, Cheah YK, Son R, Kasing A, Samuel L, Micky V, Nishibuchi M. 2011. Antibiotic resistance and plasmid profiling of Vibrio parahaemolyticus isolated from cockles (Anadara granosa) at Tanjung Karang, Kuala Selangor. Int Food Res J. 18:1183-1188.

Luo Y, Xu L, Rysz M,Wang Y, Zhang H, Alvarez PJJ. 2011. Occurrence and transport of tetracycline, sulfonamide, quinolone, and macrolide antibiotics in the Haihe River Basin, China. Environ Sci Technol. 45:1827-1833.

Malla S, Dumre SP, Shakya G, Kansakar P, Rai B, Hossain A, Nair GB, Albert MJ, Sack D, Baker S, Rahman M. 2014. The challenges and successes of implementing a sustainable antimicrobial resistance surveillance programme in Nepal. BMC Public Health. 14:269-275.

Martinez-Urtaza J, Lozano-Leon A, Vina-Feas A, de Novoa J, Garcia-Martin O. 2006. Differences in the API 20E biochemical patterns of clinical and environmental Vibrio parahaemolyticus isolates . FEMS Microbiol. Lett. 255:75-81.

Martins ML, Mouriño JLP, Fezer GF, Buglione Neto CC, Garcia P, Silva BC, Jatobá , Vieira, FN. 2010. Isolation and experimental infection with Vibrio alginolyticus in the sea horse, Hippocampus reidi Ginsburg, 1933 (Osteichthyes: Syngnathidae) in Brazil. Braz. J Biol. 70:205-209.

Mienda BS. 2012. Proteolytic activity of Vibrio parahaemolyticus isolated from Epinephilus spp. A Preliminary Report. Res Biotechnol. 3:36-40.

Mohi MM, Kuratani M, Miyazaki T, Yoshida T. 2010 Histopathological studies on Vibrio harveyi – Infected tiger puffer, Takifugu rubripes (Temminck et Schlegel), cultured in Japan. J Fish Dis. 33:833-840.

Noorlis A, Ghazali FM, Cheah YK, Tuan Zainazor TC, Wong WC, Tunung R, Nishibuchi M, Nakaguchi Y, Son R, Pui CF. 2011. Antibiotic resistance and biosafety of Vibrio cholera and Vibrio parahaemolyticus from freshwater fish at retail level. Int Food Res J. 18:59-66.

Ransangan J, Mustafa S. 2009. Identification of Vibrio harveyi isolated from diseased Asian seabass Lates calcarifer by use of 16S ribosomal DNA sequencing. J Aquatic Anim Health. 21:150-155.

Ransangan J, Imm LKL, Tamrin ML, Sade A. 2013. Phenotypic characterization and antibiotic susceptibility of Vibrio spp. isolated from aquaculture waters on the west coast of Sabah, Malaysia. Int J Res Pure Appl Microbiol. 3:58-66.

Shaw KS, Rosenberg Goldstein RE, He X, Jacobs JM, Crump BC, Sapkota AR. 2014. Antimicrobial susceptibility of Vibrio vulnificus and Vibrio parahaemolyticus recovered from recreational and commercial areas of Chesapeake Bay and Maryland coastal bays. PLoSOne 9:e89616.

Shyne Anand PS, Sobhana KS, George KC, Paulraj R. 2008. Phenotypic characteristics and antibiotic sensitivity of Vibrio parahaemolyticus strains isolated from diseased groupers (Epinephelus spp.). J Marine Biol Assoc India. 50:1-6.

Suzuki S, Hoa PTP. 2012. Distribution of quinolone, sulfonamides, tetracyclines in aquatic environment and antibiotic resistance in Indochina. Front Microbiol. 3:1-8.

Suzuki S, Ogo M, Miller TW, Shimizu A, Takada H, Siringan MA. 2013. Who possesses drug resistance genes in the aquatic environment? Sulfamethoxazole (SMX) resistance genes among the bacterial community in water environment of Metro-Manila, Philippines. Front Microbiol. 4:1-12.

You KG, Bong CW, Lee CW. 2012. Antimicrobial resistance in bacteria isolated from tropical coastal waters of Peninsular Malaysia. Malaysian J Sci. 31:111-120.

You KG, Bong CW, Lee CW. 2016. Antibiotic resistance and plasmid profiling of Vibrio spp. in tropical waters of Peninsular Malaysia. Environ. Monit Assess. 188:171.

Zulkifli Y, Alitheen NB, Raha AR, Yeap SK, Marlina SR, Nishibuchi M. 2009. Antibiotic resistance and plasmid profiling of Vibrio parahaemolyticus isolated from cockles in Padang, Indonesia. Int Food Res J. 16:53-58.


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DOI: 10.14334/Proc.Intsem.LPVT-2016-p.554-560

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