Potential Benefits from the Utilization of Leucaena leucocephala in Thai Swamp Buffaloes Production

Thongsuk Jetana


This paper aims to comply four studies of using Leucaena leucocephala as feed supplement on Thai swamp buffaloes. These studies were conducted by the Research and Development Center for Livestock Production Technology, Faculty of Veterinary Science, Chulalongkorn University, Thailand. Leucaena is interesting forage which needs further study to investigate which technology is suitable to use it as a feed for swamp buffaloes. In this review, leaves of leucaena were used as a main feed ingredient in dietary supplement. The rain tree pods (RTP) dietary supplement containing high sugar enhanced microbial supply to the small intestine. On other the hand, rain tree pod also can be used as an appetizing material for combination with leaves of leucaena, mulberry and cassia. The low fibre digestibility in dietary supplement containing leucaena may be due to tannins contents. Sodium hydroxide (NaOH) treatment and addition of polyethylene glycol (PEG) are ways to inactivate tannins contained in the leucaena. Toxicity of leucaena did not affect buffaloes, when appropriate proportion of leucaena to ruzi grass was fed. The mixed diet containing leucaena not more than 25% was the highest benefit for buffaloes as the mimosine+di-hydroxypyridine (DHP) in the urine was less 100 mg/kgBW0.75. The high proportions of RTPP (rain tree pod pellets) in leucaena increased purine derivatives (PDs) and mimosine + DHP in the urine, but none the different proportions of RTPP affects to purine derivatives/digestible organic matter (PDs/DOMI) ratios. Several studies demonstrated the approach to use leucaena as the alternative feed supplement, to enhance the quality of feeds in swamp buffaloes production improvement. The practical implication of this review, is that it would be benefit for the smallholder farmers to use leucaena for their livestock, because it is not only to improve quality of feeds and reduce cost of feed, but also higher biodiversity results in a higher N retention in soils and a lower methane releases in the atmosphere.


Leucaena leucocephala; Rain Tree Pod (Samanea saman); Rice Straw; Swamp Buffaloes


Aerts RJ, Barry TN, McNabb WC. 1999. Polyphenols and agriculture: Beneficial effects of proanthocyanidins in forages. Agric Ecosyst Environ. 75:1-12.

Alcântara PB, Ferreira TA, Beisman DA, Pereira AMF. 1997. Qualitative evaluation of DHP (di-hydroxypiridine) in the urine of buffaloes fed with leucaena. In: Proceedings XVIII international Grassland Congress, Winnipeg, Manitoba, and Saskatoon. Saskatchewan (Canada). p. 25-26.

Barry TN, Manley TR, Duncan SJ. 1986. The role of condensed tannins in the nutritional value of Lotus pedunculatus for sheep 4. Sites of carbohydrate and protein digestion as influenced by dietary reactive tannin concentration. Brit J Nutr. 55:123-137.

Brewbaker JL, Hylin JW. 1965. Variation in mimosine content among leucaena species and related mimosaceae. Crop Sci. 5:348-349.

Brooker JD, O'Donovan L, Skene I, Sellick G. 2000. Mechanisms of tannin resistance and detoxification in the rumen. In: Brooker JD, editor. Tannins in livestock and human nutrition. Proceedings of an International Workshop. Adelaide, 31th May-2nd June 1999. Canberra (Australia): ACIAR. p. 117-122.

Ghosh MK, Atreja PP, Buragohain R, Bandyopahyay S. 2007. Influence of short-term Leucaena leucocephala feeding on milk yield and its composition thyroid hormones enzyme activity and secretion of mimosine and its metabolites in milk of cattle. J Agri Sci. 145:407-414.

Hegarty MPR. Court RD, Christie GS, Lee CP. 1976. Mimosine in Leucaena leucocephala is metabolized to a goitrogen in ruminants. Aust Vet J. 52:490-495.

Hoover WH. 1986. Chemical factors involved in ruminal fiber digestion. J Dairy Sci. 62:2755-2766.

Howard H, O’Kiely P, Moloney AP, O’Mara FP. 2007. Intake, digestibility, N metabolism and growth in growing steers offered zero-grazed grass supplemented with sucrose. In: Agricultural Research Forum. Tullamore, March 12-13. Teagasc (Ireland).

Jackson MG. 1977. Review article: The alkali treatment of rice straws. Anim Feed Sci Technol. 2:105-130.

Jetana T, Abdullah N, Halim RA, Jalaludin S, Ho YW. 1998. Effects of protein and carbohydrate supplementation on fibre digestion and microbial population of sheep. Asian-Aust J Anim. Sci. 5:510-521.

Jetana T, Peetanonchai B, Thongrauy S. 2016. Effects of Leucaena leucocephala in Mixed Diets on milk production, mimosine, di-hydroxypyridine (DHP) in the urine and milk and thyroid hormones in dairy goats. Thai J Vet Med V. 25:250-251.

Jetana T, Suthikrai W, Usawang S, Vongpipatana C, Sophon S. 2006. Apparent digestion, nitrogen balance, purine derivatives and blood metabolites in male Thai, swamp buffalo and Thai Brahman cattle when fed ad libitum with rice straw. Buffalo J. 3:195-206.

Jetana T, Thongruay S, Uswang S, Hengtrakulsin R. 2012. A comparative study on mimosine, 3, 4-dihydroxy pyridine (3, 4-DHP) and 2, 3-dihydroxy pyridine (2, 3-DHP), purine derivatives (PD) excretion in the urine, thyroid hormone and blood metabolites profiles of Thai swamp buffalo (Bubalus bubalis) and Murrah buffalo (Bubalus bubalis). Trop Anim Health Prod. 44:887-897.

Jetana T, Usawang S, Vongpipatana C, Thongruay S, Sophon S. 2007. Effects of Leucaena leucocephala and Samanea saman on apparent digestibility and microbial nitrogen production in the rumen of Brahman cattle (Bos indicus) fed Pangola (Digitaria eriantha) hay as a basal diet. In: Hare MD, Wongpichet, K, editors. Proceedings of an International Forage Symposium Forages: A Pathway to Prosperity for Smallholder Farmers. Ubon Ratchathani, 5th-7th March 2007, Ubon Ratchathani (Thailand): Ubon Ratchathani University. p. 362-363.

Jetana T, Usawang S, Vongpipatana C, Thongruay S, Sophon S. 2008. Effects of replacement of leucaena (Leucaena leucocephala) with rain tree pod (Samanea saman) as a protein-rich supplement for cattle production. In: Proceedings of the 46th Kasetsart University Annual Conference. Kasetsart, 29th January-1st February 2008. Kasetsart (Thailand): Kasetsart University. p. 39-45.

Jetana T, Uswang S, Sophon S. 2010a. Using leucaena (Leucaena leucocephala) for livestock production improvement: The comparable reliability of test kit for the detection of bacteria (Synergistes jonesii) between the determination of leucaena toxic in the urine and the detection of bacteria in the rumen. In: Thai Veterinarians’ Concern for Communities. Proceedings of the 4th MUT Veterinary Annual Conferences 2010. Bangkok, 26th November 2010. Bangkok (Thailand): Faculty of Veterinary Medicine, Mahanakorn University of Technology. p. 73-81.

Jetana T, Uswang S, Thongrauy S, 2014. The effects of Leucaena leucaephala added to para grass in different proportions to form mixed diets on mimosine, di-hydroxypyridone (DHP) in urine, milk, thyroid hormones in dairy goats. In: Proceeding the 2nd Asian-Australasian Dairy Goat Conference. Bogor, 25th-27th April 2014. Bogor (Indonesia): IPB. p. 159-161.

Jetana T, Vongpipatana C, Thongruay S, Usawang S, Sophon S. 2010b. Apparent digestibility, nitrogen balance, ruminal microbial nitrogen production and blood metabolites in Thai brahman cattle fed a basal diet of rice straw and supplemented with some tropical protein-rich trees. Anim Feed Sci Technol. 23:465-474.

Jetana T, Vongpipatana C, Usawang, S, Thongruay S. 2011. The use of tropical protein-rich leaves as supplements to Thai swamp buffalo receiving a basal diet of rice straw and treated leucaena (Leucaena leucocephala). Trop Anim Health Prod. 43:57-67.

Jetana T, Soutirai W, Usawang S, Vongpipatana C, Sophon S, Liang JB. 2009. The effects of concentrate added to pineapple (Ananas comosus linn. Mer.) waste silage in differing ratios to form complete diets, on digestion, excretion of urinary purine derivatives and blood metabolites in growing, male, Thai, swamp buffaloes. Trop Anim Health Prod. 41:449-459.

Jones RJ. 1979. The value of Leucaena lecocephala as a feed for ruminants in the tropics. World Anim Rev. 31:13-23.

Khalili H, Huhtanen P. 1991. Sucrose supplements in cattle given grass silage-based diet. 1. Digestion of organic matter and nitrogen. Anim Feed Sci Technol. 33:247-261.

Klopfenstein, T.J., 1978. Chemical treatment of crop residues. J. Anim. Sci., 46, 841-848

Makkar HPS, Borowy NK, Becker K, Degen A. 1995. Some problems in fiber determination of tannins-rich forage (Acacia saline leaves) and their implications in in vivo studies. Anim Feed Sci Technol. 55:67-76.

Masafu MM, Linington MJ. 2006. The effect of feeding varying levels of Leucaena leucocephala on intake and digestibility of low-quality forages in the highveld of South Africa. Afri J Range Forage Sci. 23:177-183.

Matras J, Bartle SJ, Preston RL. 1991. Nitrogen utilization in growing lambs: Effects of grain (starch) and protein sources with various rates of ruminal degradation. J Anim Sci. 69:339-347.

McSweeney CS, Palmer B, McNeill DM, Krause DO. 2001. Microbial interactions with tannins: nutritional consequences for ruminants. Anim Feed Sci Technol. 82:227-241.

Ørskov ER. 1986. Starch digestion and utilization in ruminant. J Anim Sci. 63:1624-1633.

Phaikaew C, Suksaran W, Ted-Arsen J, Nakamanee G, Saichuer A, Seejudee S, Kotprom N, Shelton HM. 2012. Incidence of subclinical toxicity in goats and dairy cows consuming leucaena (Leucaena leucocephala) in Thailand. Anim Prod Sci. 52:283-286.

Pratchett D, Jones RJ, Syrch FX. 1991. Use of DHP-degrading rumen bacteria to overcome toxicity in cattle grains irrigated leucaena pasture. Trop Grassland. 25:268-274.

Puchala R, Kulasak GW. 1992. Estimation of microbial protein flow from the rumen of sheep using microbial nucleic acid and urinary excretion of purine derivatives. Can J Anim Sci. 712:821-830.

Shelton HM. 2009. Feeding leucaena to ruminants: How to solve problem of mimosine toxicity. In: Document of the presentation at Department of Livestock Development, 7 April 2009, Chai-Utsawaluk Building, Bangkok (Thailand): Department of Livestock Development, Ministry of Agriculture and Cooperation, Ratchatawi, Bangkok, Thailand.

Sheton HM. 2007. Adoption of tropical legume technology: An update on leucaena. In: Hare MD, Wongpichet K, editors. Forages: A Pathway to Prosperity for Smallholder Farmers. Proceedings of an International Forage Symposium. Ubon Ratchathani, 5th-7th March 2007. Ubon Ratchathani (Thailand): Ubon Ratchathani University.p. 223-247.

Silanikove N, Gilboa N, Nir I, Perevolotsky A, Nitsan Z. 1996. Effect of a daily supplementation of polyethylene glycol on intake and digestion of tannin-containing leaves (Quercus calliprinos, Pistacia lentiscus and Ceratonia siliqua) by goats. J Agri Food Chem. 44:199-205.

Staples WG, Elevitch CR. 2006. Samanea saman (rain tree) Fabaceace (legume family). In: Elevitch CR, editor. Traditional Trees of Pacific Islands: Their Culture, Environment, and Use. p. 661-674.

Stewart CS. 1977. Factors affecting the cellulolytic activity of rumen contents. Appl Environ Microb. 33:497-502.

SundstØl F, Owen E. 1984. Straw and other fibrous by-products as feed. Amsterdam (Netherlands): Elsevier Science publications.

Waghorn GC, Shelton ID. 1997. The effects of condensed tannins in Lotus pedunculatus on the nutritive value of pasture for sheep. J Agric Sci. 128:365-372.

Waghorn GC. 2008. Beneficial and detrimental effects of dietary condensed tannins for sustainable sheep and goat production-progress and challenges. Anim Feed Sci Technol. 147:116-139.

Waghorn GC, Ulyatt MJ, John A, Fisher MT. 1987. The effect of condensed tannins on the site of digestion of amino acids and other nutrients in sheep fed on Lotus corniculatus L. Brit J Nutri. 57:115-126.

Wang Y, Douglas GB, Waghorn GC, Barry TN, Foote AG, Purchas RW. 1996. Effect of condensed tannins upon the performance of lambs grazing Lotus corniculatus and Lucerne (Medicago sativa). J Agric Sci. 126: 87-98.

DOI: 10.14334/Proc.Intsem.LPVT-2016-p.90-103


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