Prevalence of Pathogenic Foodborne Bacteria from Beef in Retail Stalls in Kelantan

Fazlina Fauzi, M M Arshad, H H Ruhil, Al-Sutan II .


Salmonella, E. coli O157:H7, Methicillin-resistant Staphylococcus aureus (MRSA) and Listeria monocytogenes are common pathogenic foodborne bacteria causing foodborne illness in humans. The objective of this study was to determine the prevalence of those bacteria in beef from retail stalls in Kelantan. Beef samples were collected from retail stalls (roadside stalls and wet markets) and processed for the isolation and identification of Salmonella, E. coli O157:H7, MRSA and L. monocytogenes. Out of 25 beef samples from retail stalls, 11 (44%) were positive for Salmonella, 11 (44%) positive for E. coli and 8 (32%) positive for S. aureus. None of the beef samples contained E. coli O157:H7, MRSA and L. monocytogenes. Of 11 Salmonella isolated, the most common were S. mbandaka (46%), S. albany (36%) followed by S. weltevreden (18%). In this study, Salmonella, E. coli and S. aureus were found to be the most common foodborne bacteria from beef in retail stalls in Kelantan. Therefore, consumers have to practice a good safety food-handling during purchasing, transporting and preparing the beef in the kitchen to prevent the risk of food poisoning.


Salmonella; E. coli O157:H7; MRSA; L. monocytogenes; Beef


Ateba CN, Mbewe M. 2011. Detection of Escherichia coli O157:H7 virulence genes in isolates from beef, pork, water, human and animal species in the northwest province, South Africa: Public health implications. Res Microbiol. 162:240-248.

Bangtrakulnonth A, Pornreongwong S, Pulsrikarn C, Sawanpanyalert P, Hendriksen RS, Lo Fo Wong DM, Aerestrup FM. 2004. Salmonella serovars from humans and other sources in Thailand, 1993-2002. Emerg Infect Dis. 10:131-136.

Barkocy-Gallagher GA, Arthur TM, Rivera-Betancourt M, Nou X, Shackelford SD, Wheeler TL. 2003. Seasonal prevalence of Shiga toxin-producing Escherichia coli, including O157:H7 and non-O157 serotypes and Salmonella in commercial beef processing plants. J Food Prot. 66:1978-1986.

CDC. 2008. Infectious disease weekly report infectious disease news-local (Vol. 4). Atlanta (USA): United States Department of Health and Human Services.

CDC. 2013. Morbidity and mortality weekly report surveillance for foodborne disease outbreaks-United States, 2009-2010 (Vol. 62, pp. 41-47). typhimurium-01-13/index.html. Atlanta (USA): United States Department of Health and Human Services.

Genigeorgis CA. 1989. Present state of knowledge on Staphylococcal intoxication. Int J Food Microbiol. 9:327-360.

Hatakka M, Bjorkroth KJ, Asplud K, Maki-Petays N, Korkeala H. 2000. Genotypes and enterotoxicity of Staphylococcus aureus isolated from the hands and nasal cavities of flight catering employees. J Food Prot. 11:1487-1491.

Laws of Malaysia. 2010. Food Act 1983 (Act 281) & Regulations. Selangor (Malaysia): International Law Book Services.

Lim SK, Nam HM, Park HJ, Lee HS, Choi MJ, Jung SC. 2010. Prevalence and characterization of methicillin-resistant Staphylococcus aureus in raw meat in Korea. J Microbiol Biotechnol. 20:775-778.

MOH. 2009. Malaysia health statistics. Kuala Lumpur (Malaysia): Ministry of Health Malaysia

MOH. 2010. Health facts 2009: Ministry of Health Malaysia

MOH. 2011. Health facts 2011: Ministry of Health Malaysia

MOH. 2013. Health facts 2013: Ministry of Health Malaysia

Mohammed HO, Atwill E, Dunbar L, Ward T, McDonough P, Gonzalez R, Stipetic K. 2010. The risk of Listeria monocytogenes infection in beef cattle operations. J Appl Microbiol. 108:349-356.

Norton DM, Braden CR. 2007 Foodborne listeriosis. In: Listeria, Listeriosis, and Food Safety ed. Ryse ET, Marth E, editors. Boca Raton (USA): CRC press. p. 305-356.

Okutani A, Okada Y, Yamamoto S, Igimi S, 2004. Nationwide survey of human Listeria monocytogenes infection in Japan. Epidemiol Infect. 132:769-772.

Quinn PJ, Markey BK, Donnelly WJ, Leonard FC. 2002. Veterinary microbiology and microbial disease. Oxford (UK): Blackwell Publishing.

Scallan E, Hoekstra RM, Angulo FJ, Tauxe RV, Widdowson MA, Roy SL. 2011. Foodborne illness acquired in the United States-major pathogens. Emerg Infect Dis. 17:7-15.

Scherrer D, Corti S, Muehlherr JE, Zweifel C, Stephen R. 2004. Phenotypic and genotypic characteristics of Staphylococcus aureus isolates from raw bulk-tank milk samples of goats and sheep. Vet Microbiol. 101:101-107.

Thong KL, Goh YL, Radu S, Noorzaleha S, Yasin R, Koh YT. 2002. Genetic diversity of clinical and environmental strains of Salmonella enterica serotype Weltevreden isolated in Malaysia. J Clin Microbiol. 40:498-503.

Vo ATT, Duijkeren EV, Fluit AC, Heck MEOC, Verbruggen A, Maas HME, Gaastra W. 2006. Distribution of Salmonella enterica serovars from humans, livestock and meat in Vietnam and the dominance of Salmonella typhimurium phage type 90. Vet Microbiol. 113:153-158.

Wray C, Wray A. 2000. Salmonella in domestic animals. New York (USA): CABI Publishing.

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DOI: 10.14334/Proc.Intsem.LPVT-2016-p.526-531


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