Evaluation of Using Hibiscus sabdariffa Calyces in the Production of Rotenesse Smoked Beef (Se’i)

Gemini EM Malelak, G M Sipahelut, I GN Jelantik

Abstract


Aims of this experiment were to evaluate the use of Hibiscus sabdariffa extract on the production of se’i (Rotenesse smoked beef). A completely randomized design was used in this study. Treatments include: Po = se’i was without Hibiscus sabdariffa extract as control, P1 = se’i given Hibiscus sabdariffa extract 1% (w/v); P2 = se’i was given Hibiscus sabdariffa extract 2% (w/v); P3 = se’i was given Hibiscus sabdariffa extract 3% (w/v); P4 = se’i was given Hibiscus sabdariffa extract 4% (w/v); and P5 = se’i was given Hibiscus sabdariffa extract 5% (w/v), each treatment had three replication. Variables for this experiment include: flavour, color, taste, tenderness, pH, total plate count, Coliform, Escherichia coli, Salmonella and Staphylococcus aureus. Result showed that Hibiscus sabdariffa extract was not significantly affected the flavour, color, tenderness and pH. However, Hibiscus sabdariffa extract enhanced the taste of se’i (P<0.05). Also, the extract significantly reduced both total plate count and Staphylococcus aureus numbers (P< 0.05). As a matter of fact, Coliform, Escherichia coli and Salmonella were not detected in all se’i. In conclusion Hibricus sabdariffa was able to enhanched the taste of se’i  and inhibit the growth of bacteria.

Keywords


Se’i; Hibiscus sabdariffa; Organoleptics; Bacterial

References


Bensink JC, Ford AL, Yates JR. 1973. Properties and performance of a range of commercial vacuum packaging films used for packing chilled beef. Meat Research Report. 4/73. p.1-1.

Bozkurt H, Belibagl KB. 2009. Use of Rosemary and Hibiscus sabdariffa L in production of Kavurma, a cooked meat product. J Sci Food Agric. 89:1168-1173.

Christian KR, Jackson. 2009 JC. Changes in total phenolic and monomeric anthocyanin composition and antioxidant activity of three varieties of sorrel (Hibiscus sabdariffa) during maturity. J Food Comp Anal. 22:663-667.

Chao CY, MC. Yin.2009. Antibacterial effects of Roselle Calyx extracts and protocatechuic acid in ground beef and apple juice. Foodborne Pathog Dis. 6:201-206.

Cisse M, Dornier M, Sakho M, Ndiaye, A, Reynes M, Sock O. 2009. Le bissap (Hibiscus sabdariffa L.): Composition et principales utilisations. Fruits. 64:179-193.

Cowan MM. 1999. Plant products as antimicrobial agents. Clin Microbial Rev. 12:564-582.

Elliot RP, Clark KH, Lewis H, Lundbeck JC, Olson JR, Simonsen B. 1978. Microorganisms in Food 1. Their Significance and Methods of Enumeration. 2nd ed. Toronto (Canada): University of Toronto Press.

Fullerton M, Khatiwada J, Johnson JU, Davis S, Williams LL. 2011. Determination of antimicrobial activity of sorrel (Hibiscus sabdariffa) on Esherichia coli O157:H7 isolated from food, veterinary, and clinical samples. J Med Food. 14:950-956.

Harrigan WF, McCance ME. 1976. Laboratory methods in food and dairy microbiology. London (UK): Academic Press. p. 452.

Karabacak S, Bozkurt H. 2008. Effects of Urtica dioca and Hibiscus sabdariffa on the quality and safety of sucuk (Turkish dry-fermented sausage). Meat Sci. 78:288-296.

Lin TL, Lin HH, Chen CC, Lin MC, Chou MC, Wang CJ. 2007. Hibiscus sabdariffa extract reduces serum cholesterol in men and women. Nut Res. 27:140-145.

Medvedova A, Valik L. 2012. Staphylococcus aureus: Characterization and quantitative growth description in milk and artisanal raw milk cheese production. In: Eissa AA, editor. Intech. Available from http://www.Intechopen.com/books/structure-and-function-of-food-engineering.

Morales-Cabrera M, Hernandez-Morales J, Leyva-Ruelas G, Salinas-Moreno Y, Soto-Rojas L, Castro-Rosas J. 2013. Influence of variety and extraction solvent on antibacterial activity of roselle (Hibiscus sabdariffa L.) calyces. J Med Plants Res. 7:2319-22.

Nwaiwu NE, Mshelia F, Raufu IA .012. Antimicrobial activities of crude extracts of Moringa Oleifera, Hibiscus S and Hibiscus Esculentus seeds against some enterobacteria. J Appl Phytotechnol Environ Sanit. 1:11-16.

Olaleye M. T. 2007. Cytotoxicity and antibacterial activity of methanolic extract of Hibiscus sabdariffa. J Med Plant Res. 1:9-13.

Samelis J, Sofos J. 2000. Organic acids. In: Roller S, editor. Natural antimicrobials for the minimal processing of foods. CRC, Cambridge UK. Great Abington (UK): Woodhead Publishing Ltd.

SNI. 2009. Maximum Limited of Microbial Contamination of food. SNI: 7388: 2009. Jakarta (Indonesia): Indonesian National Standard.


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DOI: 10.14334/Proc.Intsem.LPVT-2016-p.514-519

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