Fermentative Gas Production of Different Feeds Collected During Wet and Dry Seasons when Incubated with Rumen Fluid from Timor Deer (Cervus timorensis)

Muhammad Sadik Arifuddin, R Utomo, H Hartadi, Darmy .


An experiment was done to investigate rumen microbial fermentation of various feeds comonly given to Timor deer (Cervus timorensis) using the in vitro gas production technique. Seven forage feed sample (Sesbania grandiflora, Leucaena leucocephala, Glyricidia sepium, Zea mays, Ipomea aquatica, Pennisetum purpureum, and native grass) collected during dry and wet seasons and three concentrate feed samples (rice bran, copra meal and tofu waste) were dried and ground. The feed sample (200 g) was transferred into incubation syringe which was then added with incubation medium (mixture of rumen fluid and buffer solution). The rumen fluid was collected from 2 Timor deer using a trokar technique. Incubation was run for 72 h, and gas production was read at 2, 4, 6, 12, 24, 48, and 72 h of incubation. Data were fitted to an exponential equation generally used in similar in vitro gas production studies. Results of the study indicated that there was an effect of season on fermentation parameters (values of a, b or c) for the same feed, but this effect was not consistent from one feed to another. From data of total and cummulative gas productions, it was indicated that Zea mays in either dry or wet season showed the highest, while Leucaena leucocephala exhibited the lowest gas production compared to other feeds tested.


Cervus Timorensis; Gas Poduction; Wet Season; Dry Season; Fermentation


Chen BX. 1995. Neway exel: An axel application programme for processing feed degradability data. User Manual. Aberdeen (UK): International Feed Resources Unit, Rowet Research Institute Bucksburn.

McDonald P, Edwards RA, Greenhalgh JFD, Morgan CA. 2002. Animal Nutrition. Edinburgh Gate (UK): Pearson Education Ltd.

Menke KH, H Steingass. 1988. Estimation of energetic feed value obtained from chemical analysis and in vitro gas production. Anim Res Dev. 28:7-25.

Orskov ER, Ryle M. 1990. Energy nutrition in ruminants. Elsevier Applied Science, London and New York.

Orskov ER, McDonald I. 1979. The estimation of protein degradability in the rumen from incubation measurements weighted according to rate of passage. J Agric Sci. 92:499-503.

Theodorou MK, Williams BA, Dhanoa MS, McAllen ADB, France J. 1994. A simple gas preduction method using a pressure tranducer to determine the fermentation kinetics of rumen fluids. Anim Feed Sci Technol. 48:185-197.

Full Text: PDF

DOI: 10.14334/Proc.Intsem.LPVT-2016-p.495-501


  • There are currently no refbacks.