Polymorphisms of Bovine Lymphocyte Antigen (BoLA DRB 3.2 Locus PstI) Gene in Bali Cattle Population from South Sulawesi Province

Andi Baso L Ishak, MIA Dagong, P Nurul


Immune Response in cattle is determined by polymorphism of major histocompatibility complex (MHC) gene mainly Bovine Lymphocyte Antigen. The aim of this study is to identify the genetic polymorphism of Bovine Lymphocyte Antigen (BoLA DRB.3) gene exon 2 as a marker for infectious disease resistance of Bali cattle in South Sulawesi, Indonesia. A total of 200 blood samples were extracted for DNA templated. It was amplified by using a PCR-RFLP method and digested with Pst1 restriction enzyme. Restriction of PCR product by Pst1 enzyme represented two digested fragments. The pp homozygous genotype showed two fragment 270 and 15 bp (not demonstrated). Pp heterozygous genotype showed four fragment 270, 226, 44 (not demonstrated), and 15 bp (not demonstrated). Reffering from this result this research, the frequency of allele P (0.925), allele p (0.075). The frequency genotype of PP (0.850), frequency of genotype Pp (0.150), polymorphism information content (0.129). Heterozygosity observed (0.150) and heterosygosity expected (0.139).


Bali Cattle; MHC BoLA Gene; PCR-RLFP


Andersson L, Lundén A, Sigurdardóttir S, Davies CJ, Rask L. 1988. Linkage relationships in the bovine MHC region. High recombination frequency between class II subregions. Immunogenetics. 27:273-280.

Bastos-Silveira C, Luís C, Ginja C, Gama LT, Oom MM. 2008. Genetic variation in BoLA microsatellite loci in Portuguese cattle breeds. Anim Genet. 40:101-105.

Davies CJ, Andersson L, Ellis SA, Hensen EJ, Lewin HA, Mikko S, Muggli-Cockett NE, Van der Poel JJ, Russell GC. 1997. Nomenclature for factors of the BoLA system, 1996: Report of the ISAG BoLA nomenclature committee. Anim Genet. 28:159-168.

Martojo H. 2012. Indigenous Bali cattle is most suitable for sustainable small farming in Indonesia. Reprod Dom Anim. 47:10-14.

Miretti MM, Ferro JA, Lara MA, Contel EPB. 2001. Restriction fragment length polymorphism (RFLP) in exon 2 of the BoLA-DRB3 gene in South American cattle. Biochem Genet. 39:311-324.

Mohammad K, Olsson M, Helena TA, Mikko S, Vlamings BH, Andersson G, Martınez HR, Purwantara B, Paling RW, Colenbrander B, Lenstra JA. 2009. On the origin of Indonesian cattle. PlosOne. 4:1-6.

Nei M, Kumar S. 2000. Molecular evolution and phylogenetics. New York (US): Oxford University Press.

Ningtiyas WD, Dagong MIA, Rahim L, Bugiwaty SRA, Ishak ABL. 2014. Allelic variation of MHC DRB3 gene in Bali and crossbred cattle from South Sulawesi, Indonesia. In: Subandriyo, Kusmartono, Santosa KA, Kurnianto E, Purnomoadi A, Sodiq A, Wiryawan KG, Darodjah S, Inounu I, Darmono, Priyanti A, Wynn P, han JL, Tay-Hsu J, Idrus Z, editors. Sustainable Livestock Production in the Perspective of Food Security, Policy, Genetic Resources, and Climate Cange. Proceedings of the 16th AAAP Animal Science Congress Vol. II. Yogyakarta, 10-14 November 2014. Yogyakarta (Indonesia): Ministry of Agriculture, Indonesian Society of Animal Sciences, Gadjah Mada University. p. 97-100

Noor RR, 2008. Genetika ternak. 4th Ed. Bogor (Indonesia): Penebar Swadaya.

Purwantara B, Noor RR, G Andersson G, Martinez HR. 2012. Banteng and Bali cattle in Indonesia: Status and forecasts. Reprod Dom Anim. 47:2-6.

Puja IK, Wandia IN, Suastika P, Sulabda IN. 2011. Polimorfisme genetik DNA mikrosatellite GEN BoLA lokus DRB3 pada sapi Bali (Bos indicus). Biota. 16:336-341.

Ripoli MV, Lir JP, De Luca JC, Rojas F, Dulout FN, Giovambattista G. 2004. Gene frequency distribution of the BoLA-DRB3 locus in Saavedreno Creole dairy cattle. Biochem Genet. 42:231-240.

Ruzina MN, Shtyfurkoa TA, Mohammadabadi MR, Gendzhievac OB, Tsedevd T. 2010. Polymorphism of the BoLA DRB3 gene in the Mongolian, Kalmyk, and Yakut cattle breeds. Russ J Genet. 46:456-463.

Wu XX, Yang ZP, Wang XL, Mao YJ, Li SC, Shi XK, Chen Y. 2010. Restriction fragment length polymorphism in the exon 2 of the BoLA-DRB3 gene in Chinese Holstein of the South China. J Biomed Sci Eng. 3:221-225

Sambrook J, Russell DW. 2001. Molecular cloning: A laboratory manual. 3rd Ed. New York (US): Cold Spring Harbor Laboratory Press.

Full Text: PDF

DOI: 10.14334/Proc.Intsem.LPVT-2016-p.19-23


  • There are currently no refbacks.