Genetic Variants of Milk Protein Genes and Their Association with Milk Components in Holstein Friesian Cattle

Santiananda Arta Asmarasari, Cece Sumantri, A Gunawan, E Taufik, Anneke Anggraeni

Abstract

Protein content in milk is an important indicator of milk. Accordingly, genetic improvement to produce Holstein Friesian (HF) dairy cattle is important. The objective of this study was to evaluate the genetic variant of milk protein genes and its effect on milk component traits of Holstein Friesian (HF). A total of 100 HF were used in this study. The HF cattle used have physiological status in the lactation period 1 up to 3 and lactation change of 1 up to 12 months. Genotype variants of milk protein genes were identified using Real Time-Polymerase Chain Reaction method.  Analysis of milk component was carried out covering the component of protein, fat, lactose, and solid non-fat (SNF) by using a milk quality measuring device (Lactoscan). Genotyping of cattle blood samples consisted of DNA extraction, genes amplification using the RT-PCR method. The result showed that protein milk was significantly affected (p<0.05) by the genetic variants of CSN1S1-192 and CSN2-67 genes. Fat milk was significantly affected (p<0.05) by the genetic variants of CSN1S1-192 and CSN3 genes.  Meanwhile, solid non-fat milk was significantly affected (p<0.05) by the genetic variants of CSN-BMC9215, CSN-BMC6334, CSN1S1-14618, CSN2_67, and CSN3 genes. Lactose milk was significantly affected (p<0.05) by the genetic variants of CSN-BMC9215 and CSN2-67 genes. It was concluded that genetic variants of the milk protein genes have an association with the component of cow's milk (protein, fat, solid non-fat, and lactose).

Keywords

Genetic Variant, Protein Genes, Milk Component

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References

Anggraeni A, Anneke A, Nury HS, Andreas E, Sumantri C. 2017. Genetic variants of k-casein and β-lactoglobulin genes and their association with protein and milk components of holstein friesian cows at small farmers in Lembang, West Java. KnE Life Sci. 2:86–94.

Ardicli S, Soyudal B, Samli H, Dincel D, Balci F. 2018. Effect of STAT1, OLR1, CSN1S1, CSN1S2 , and DGAT1 genes on milk yield and composition traits of Holstein breed. Rev Bras Zootec. 47:e20170247.

Barbosa SBP, Araújo ÍIM de, Martins MF, Silva EC da, Jacopini LA, Batista ÂMV, Silva MVB da. 2019. Genetic association of variations in the kappa-casein and β-lactoglobulin genes with milk traits in girolando cattle. Rev Bras Saúde e Produção Anim. 20:e0312019.

Bhat SA, Ahmad SM, Ibeagha-Awemu EM, Bhat BA, Dar MA, Mumtaz PT, Shah RA, Ganai NA. 2019. Comparative transcriptome analysis of mammary epithelial cells at different stages of lactation reveals wide differences in gene expression and pathways regulating milk synthesis between Jersey and Kashmiri cattle.Loor JJ, editor. PLoS One. 14:e0211773.

Bonfatti V, Di Martino G, Cecchinato A, Vicario D, Carnier P. 2010. Effects of β-κ-casein (CSN2-CSN3) haplotypes and β-lactoglobulin (BLG) genotypes on milk production traits and detailed protein composition of individual milk of Simmental cows. J Dairy Sci. 93:3797–3808.

Brka M, Hodžić A, Reinsch N, Zečević E, Dokso A, Djedović R, Rukavina D, Kapur L, Vegara M, Šabanović M, Ravić I. 2010. Polymorphism of the kappa-casein gene in two Bosnian autochthonous cattle breeds. Arch Anim Breed. 53:277–282.

Bugeac T, Bâlteanu V, Creanga S. 2013. Kappa-casein genetic variants and their relationships with milk production and quality in Montbéliarde dairy cows. Bull UASVM Anim Sci Biotechnol. 70:193–194.

Caroli AM, Chessa S, Erhardt GJ. 2009. Invited review: Milk protein polymorphisms in cattle: Effect on animal breeding and human nutrition. J Dairy Sci. 92:5335–5352.

Cebo C, Lopez C, Henry C, Beauvallet C, Ménard O, Bevilacqua C, Bouvier F, Caillat H, Martin P. 2012. Goat αs1-casein genotype affects milk fat globule physicochemical properties and the composition of the milk fat globule membrane. J Dairy Sci. 95:6215–6229.

Cecchinato A, Bobbo T, Ruegg PL, Gallo L, Bittante G, Pegolo S. 2018. Genetic variation in serum protein pattern and blood β-hydroxybutyrate and their relationships with udder health traits, protein profile, and cheese-making properties in Holstein cows. J Dairy Sci. 101:11108–11119.

Çobanoglu Ö, Gurcan EK, Çankaya S, Kul E, Abaci SH, Ülker M. 2016. Effects of lactation month and season on test-day milkyield and milk components in Holstein cows. Indian J Anim Res. 51:952–955.

Dagnachew B, Georg T, Lien S, Ådnøy T. 2011. Casein SNP in Norwegian goats: additive and dominance effects on milk composition and quality. Genet Sel Evol. 43:1–12.

Davoodi SH, Shahbazi R, Esmaeili S, Sohrabvandi S, Mortazavian AM, Jazayeri S, Talimi A. 2016. Health-related aspects of milk proteins. Iran J Pharm Res. 15:573–591.

Deb R, Singh U, Kumar S, Singh R, Sengar G, Sharma A. 2014. Genetic polymorphism and association of kappa-casein gene with milk production traits among Frieswal (HF × Sahiwal) cross breed of Indian origin. Iran J Ver Res. 15:406–408.

Djedovic R, Bogdanovic V, Perisic P, Stanojevic D, Popovic J, Brka M. 2015. Relationship between genetic polymorphism of κ-casein and quantitative milk yield traits in cattle breeds and crossbreds in Serbia. Genetika. 47:23–32.

Farrell HM, Jimenez-Flores R, Bleck GT, Brown EM, Butler JE, Creamer LK, Hicks CL, Hollar CM, Ng-Kwai-Hang KF, Swaisgood HE. 2004. Nomenclature of the Proteins of Cows’ Milk—Sixth Revision. J Dairy Sci. 87:1641–1674.

Ferretti L, Leone P, Sgaramella V. 1990. Long range restriction analysis of the bovine casein genes. Nucleic Acids Res. 18:6829–6833.

Fleming A, Schenkel FS, Chen J, Malchiodi F, Ali RA, Mallard B, Sargolzaei M, Corredig M, Miglior F. 2017. Variation in fat globule size in bovine milk and its prediction using mid-infrared spectroscopy. J Dairy Sci. 100:1640–1649.

Franzoi M, Niero G, Visentin G, Penasa M, Cassandro M, De Marchi M. 2019. Variation of Detailed Protein Composition of Cow Milk Predicted from a Large Database of Mid-Infrared Spectra. Animals. 9:176.

Gurmessa J, Melaku A. 2012. Effect of lactation stage, pregnancy, parity and age on yield and major components of raw milk in bred cross Holstein Friesian Cows. World J Dairy Food Sci. 7:146–149.

Gurses M, Yuce H. 2012. Determination of kappa casein gene polymorphisms and their effects on milk composition in some native cattle breeds of Turkey. J Anim Vet Adv. 11:1023–1027.

Hamza A, Yang Z, Wang X, Chen R, Wu H, Ibrahim A. 2011. The impact of kappa casein gene polymorphism on milk components and other productive performance traits of Chinese Holstein cattle. Pak Vet J. 31:153–156.

Hristov O, Teofanova D, Radoslavov G. 2011. Effects of genetic variants of milk protein genes on milk composition and milk yield in cows of the bulgarian black pied cattle. Compt rend Acad bulg Sci. 64:75–80.

Huang W, Peñagaricano F, Ahmad K, Lucey J, Weigel K, Khatib H. 2012. Association between milk protein gene variants and protein composition traits in dairy cattle. J Dairy Sci. 95:440–449.

Januś E, Borkowska D. 2011. Effect of selected factors on milk energy value of cow’s milk from PHF BW and Montbeliarde breeds. ŻYWNOŚĆ Nauk Technol Jakość. 5:141–149.

Jónás E, Atasever S, Gráff F, Erdem H. 2016. Non-genetic factors affecting milk yield, composition and somatic cell count in Hungarian Holstein cows. Kafkas Univ Vet Fak Derg. 22:361–366.

Khaizaran ZA, Al-Razem F. 2014. Analysis of selected milk traits in Palestinian Holstein-Friesian cattle in relation to genetic polymorphism. J Cell Anim Biol. 8:74–85.

Komori R, Kobayashi T, Matsuo H, Kino K, Miyazawa H. 2013. Csn3 gene is regulated by all-trans retinoic acid during neural differentiation in mouse P19 cells.Cooney AJ, editor. PLoS One. 8:e61938.

Laible G, Smolenski G, Wheeler T, Brophy B. 2016. Increased gene dosage for β- and κ-casein in transgenic cattle improves milk composition through complex effects. Sci Rep. 6:37607.

Le Parc A, Leonil J, Chanat E. 2010. αS1-casein, which is essential for efficient ER-to-Golgi casein transport, is also present in a tightly membrane-associated form. BMC Cell Biol. 11:65.

Mangia NP, Saliba L, Zoumpopoulou G, Chessa S, Anastasiou R, Karayiannis Ι, Sgouras D, Tsakalidou E, Nudda A. 2019. Goat milk with different alpha-s1 casein genotype (CSN1S1) fermented by selected Lactobacillus paracasei as potential functional food. Fermentation. 5:55.

Mohammadi Y, Aslaminejad AA, Nassiri MR, Koshkoieh AE. 2013. Allelic polymorphism of K-casein, b-lactoglobulin and leptin genes and their association with milk production traits in Iranian Holstein cattle. J Cell Mol Res. 5:75–80.

Morkūnienė K, Baltrėnaitė L, Puišytė A, Bižienė R, Pečiulaitienė N, Makštutienė N, Mišeikienė R, Miceikienė I, Kerzienė S. 2016. Association of kappa casein polymorphism with milk yield and milk protein genomic values in cows reared in Lithuania. Vet Med Zoot. 74:27–32.

Ohlsson JA, Johansson M, Hansson H, Abrahamson A, Byberg L, Smedman A, Lindmark-Månsson H, Lundh Å. 2017. Lactose, glucose and galactose content in milk, fermented milk and lactose-free milk products. Int Dairy J. 73:151–154.

Olenski K, Kamiński S, Szyda J, Cieslinska A. 2010. Polymorphism of the beta-casein gene and its associations with breeding value for production traits of Holstein–Friesian bulls. Livest Sci. 131:137–140.

Ozdemir M, Kopuzlu S, Topal M, Bilgin OC. 2018. Relationships between milk protein polymorphisms and production traits in cattle: a systematic review and meta-analysis. Arch Anim Breed. 61:197–206.

Radhika G, Ajithkumar S. 2018. Low solids not fat percentage in milk of crossbred cows in wayanad district of kerala, india – a retrospective study. Int J Sci Environ. 7:1962–1969.

Ren D, Miao S, Chen Y, Zou C, Liang X, Liu J. 2013. Genotyping of the κ-casein and βlactoglobulin genes in Chinese Holstein, Jersey and water buffalo by PCR-RFLP. J Genet. 92:1–5.

Salamonczyk E. 2013. Cow’s milk quality and energy value during different lactation stages. Acta Sci Pol Technol Aliment. 12:303–310.

Schopen GCB, Visker MHPW, Koks PD, Mullaart E, van Arendonk JAM, Bovenhuis H. 2011. Whole-genome association study for milk protein composition in dairy cattle. J Dairy Sci. 94:3148–3158.

Sigl T, Meyer HHD, Wiedemann S. 2012. &nbsp;Gene expression of six major milk proteins in primary bovine mammary epithelial cells isolated from milk during the first twenty weeks of lactation. Czech J Anim Sci. 57:469–480.

Stoop WM, Bovenhuis H, Heck JML, van Arendonk JAM. 2009. Effect of lactation stage and energy status on milk fat composition of Holstein-Friesian cows. J Dairy Sci. 92:1469–1478.

Sudhakar K, Panneerselvam S, Thiruvenkadan A, Abraham J, Vinodkumar G. 2013. Factors affecting milk fat percentage and solids-not-fat percentage and milk price of dairy cattle in humid tropics. Int J Food, Agric Vet Sci. 3:229–233.

Thomas M, Sasidharan M. 2015. Factors affecting milk fat percentage and solids-not-fat percentage and milk price of dairy cattle in humid tropics. Adv Agric Sci. 3:11–17.

Trakovickà A, Moravčikovà N, Navràtilovà A. 2012. Kappa-casein gene polymorphism (CSN3) and its effect on milk production traits. Acta Fytotech Zootech. 3:61–64.

Vidović V, Nemes Z, Popović-Vranjes A, Lucač D, Cvetanovic D, Strbac L, Stupar M. 2013. Heritability and correlations of milk traits in the view of kappa-casein genotypes in Vojvodina Holstein-Friesian dairy cattle. Mljekarstvo. 63:91–97.

Volkandari SD, Indriawati I, Margawati ET. 2017. Genetic polymorphism of kappa-casein gene in Friesian

Holstein: a basic selection of dairy cattle superiority. J Indones Trop Anim Agric. 42:213–219.

Zepeda-Batista JL, Alarcón-Zúñiga B, Ruíz-Flores A, Núñez-Domínguez R, Ramírez-Valverde R. 2015. Polymorphism of three milk protein genes in Mexican Jersey cattle. Electron J Biotechnol. 18:1–4.

Zhou C, Li C, Cai W, Liu S, Yin H, Shi S, Zhang Q, Zhang S. 2019. Genome-wide association study for milk protein composition traits in a chinese holstein population using a single-step approach. Front Genet. 10:72.

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