Condition of Rumen Fermentation as Impacted by Supplementation of Fermented Rice Brand Using In Vitro Gas Production Technique

Zuratih Zuratih, SPS Budhi, Z Bachruddin


Methane is one of the gases produced by ruminants during feed fermentation in the rumen. This experiment was aimed to investigate the production of monacolin K in rice bran fermented by Monascus purpureus mold and the influence of the supplementation of fermented rice bran using Monascus purpureus mold on elephant grass basal diet on fermentation products and methane production in an in vitro gas production method. The study consisted of two experiments. The first experiment analysis of monacolin K production in fermented rice bran using Monascus purpureus. Fermentation is done by the addition of Monascus purpureus at levels 0, 4, 8, and 12% (v/w) of substrate (rice bran) with 3 replications. Monacolin K in the substrate was analyzed using HPLC. The second experiment was the evaluation of supplementation of fermented rice bran to elephant grass basal diet using in vitro gas production. The treatment diet evaluated were Pennisetum purpureum (control), Pennisetum purpureum:rice bran (1:1 ratio), and Pennisetum purpureum:rice bran fermented. Each treatment was replicated 3 times. Results from the first experiment shows that rice bran with the highest monacolin K content was in rice bran fermented at 12% by Monascus purpureus. Result from the second experiment showed that supplementation of fermented rice bran to Pennisetum purpureum basal diet did not affect rumen ammonia concentration, VFA, protein microbial production, and dry matter and organic matter digestibility. However, methane production (CH4) was reduced (P<0.05) by 50%, and the protozoal population was decreased (P<0.05) by 80%. It is concluded that supplementation of fermented rice brands containing monacolin K was able to reduce methane production and the protozoa population without affecting feed fermentation.


Fermentasi, In Vitro, Metana, Monakolin K, Monascus purpureus

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AOAC. 2006. Official Methods of Analysis. 18th Ed. Gaithersburg (MD): Association of Official Analytical Chemists.

Beltowski J, Wojcicka G, Jamroz-Wisniewska A. 2009. Adverse effects of statins-mechanisms and consequences. Curr Drug Saf. 4:209–228.

Benchaar C, Greathead H. 2011. Essential oils and opportunities to mitigate enteric methane emissions from ruminants. Anim Feed Sci Technol. 166–167:338–355.

Bodas R, Prieto N, García-González R, Andrés S, Giráldez FJ, López S. 2012. Manipulation of rumen fermentation and methane production with plant secondary metabolites. Anim Feed Sci Technol. 176:78–93.

Candyrine SCL, Mahadzir MF, Garba S, Jahromi MF, Ebrahimi M, Goh YM, Samsudin AA, Sazili AQ, Chen WL, Ganesh S, et al. 2018. Effects of naturally-produced lovastatin on feed digestibility, rumen fermentation, microbiota and methane emissions in goats over a 12-week treatment period. PLoS One. 13:1–19.

Chaney AL, Marbach EP. 1962. Modified reagents for determination of urea and ammonia. Clin Chem. 8:130–132.

Diaz A, Avendano M, Escobar A. 1993. Evaluation of Sapindus saponaria as a defaunating agent and its effects on different ruminal digestion parameters. Res Rural Dev. 5.

Dinesh N, Pallerla DSR, Kaur PK, Kishore Babu N, Singh S. 2014. Exploring Leishmania donovani 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR) as a potential drug target by biochemical, biophysical and inhibition studies. Microb Pathog. 66:14–23.

Filípek J, Dvořák R. 2009. Determination of the volatile fatty acid content in the rumen liquid: Comparison of gas chromatography and capillary isotachophoresis. Acta Vet Brno. 78:627-633.

Goldberg AL. 2013. Protein Degradation. In: Lennarz WJ, Lane MD. Encycl Biol Chem. 2nd Ed. Amsterdam (NL): Elsevier. p. 617–624.

Halliwell G, Lovelady J. 1981. Utilization of carboxymethylcellulose and enzyme synthesis by Trichoderma koningii. J Gen Microbiol. 126:211–217.

Karlsson L, Hetta M, Udén P, Martinsson K. 2009. New methodology for estimating rumen protein degradation using the in vitro gas production technique. Anim Feed Sci Technol. 153: 193-202.

Liu J, Luo Y, Guo T, Tang C, Chai X, Zhao W, Bai J, Lin Q. 2020. Cost-effective pigment production by Monascus purpureus using rice straw hydrolysate as substrate in submerged fermentation. J Biosci Bioeng. 129:229–236.

Martin C, Morgavi DP, Doreau M. 2010. Methane mitigation in ruminants: From microbe to the farm scale. Animal. 4:351–365.

Martínez ME, Ranilla MJ, Tejido ML, Saro C, Carro MD. 2010. Comparison of fermentation of diets of variable composition and microbial populations in the rumen of sheep and Rusitec fermenters. II. Protozoa population and diversity of bacterial communities. J Dairy Sci. 93:3699–3712.

Menke KH, Steingass H. 1988. Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid. Anim ßes Dev. 28:7–55.

Morgavi DP, Martin C, Boudra H. 2013. Fungal secondary metabolites from Monascus spp. reduce rumen methane production in vitro and in vivo1. J Anim Sci. 91:848–860.

Olijhoek DW, Hellwing ALF, Brask M, Weisbjerg MR, Højberg O, Larsen MK, Dijkstra J, Erlandsen EJ, Lund P. 2016. Effect of dietary nitrate level on enteric methane production, hydrogen emission, rumen fermentation, and nutrient digestibility in dairy cows. J Dairy Sci. 99:6191–6205.

Plummer DT. 1987. An Introduction to Practical Biochemistry. London (UK): McGraw-Hill Book.

Sharpe LJ, Brown AJ. 2013a. Controlling cholesterol synthesis beyond 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR). J Biol Chem. 288:18707-18715.

Sharpe LJ, Brown AJ. 2013b. Controlling Cholesterol Synthesis beyond 3-Hydroxy-3-methylglutaryl-CoA Reductase (HMGCR). J Biol Chem. 288:18707–18715.

Shi Y-C, Pan T-M. 2011. Beneficial effects of Monascus purpureus NTU 568-fermented products: a review. Appl Microbiol Biotechnol. 90:1207–1217.

Sitoresmi PD, Yusiati LM, Hartadi H, Peternakan F, Mada UG, No JF. 2009. Pengaruh penambahan minyak kelapa, minyak biji bunga matahari, dan minyak kelapa sawit terhadap penurunan produksi metan di dalam rumen secara. Bul Peternakan. 33:96–105.

Su YC, Wang JJ, Lin TT, Pan TM. 2003. Production of the secondary metabolites γ-aminobutyric acid and monacolin K by Monascus. J Ind Microbiol Biotechnol. 30:41–46.

Suharti S, Aliyah DN, Suryahadi S. 2019. Karakteristik fermentasi rumen in vitro dengan penambahan sabun kalsium minyak nabati pada buffer yang berbeda. J Ilmu Nutr Teknol Pakan. 16: 56-64.

Suryani NIN, Mangku IK, Dan B, Ari IP. 2014. Fermentasi rumen dan sintesis protein mikroba kambing peranakan ettawa yang diberi pakan dengan komposisi hijauan beragam dan level konsentrat berbeda. Maj Ilm Peternak. 17:56–60.

Widiawati Y, Rofiq MN, Tiesnamurti B. 2016. Methane emission factors for enteric fermentation in beef cattle using IPCC Tier-2 method in Indonesia. JITV. 21:101-111.

Zhang BB, Lu LP, Xia Y jun, Wang YL, Xu GR. 2013. Use of agar as carrier in solid-state fermentation for Monacolin K production by Monascus: A novel method for direct determination of biomass and accurate comparison with submerged fermentation. Biochem Eng J. 80:10–13.


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