Optimization of BS4 Enzyme production with Different Substrate Thickness and Type of Trays

Tuti Haryati, Arnold P Sinurat, H Hamid, Tresnawati Purwadaria


BS4 enzyme that is produced from solid substrate fermentation (SSF) on coconut cake with Eupenicillium javanicum BS4 in tray bioreactor has been applied as a feed additive. It increases the nutritional value of animal feedstuff. The BS4 production on SSF may be influenced by the better aeration through the perforated trays or by the thinner substrate. The aim of this research is to optimize the production of BS4 with different substrate thicknesses and types of trays. The trial was carried out using a factorial randomized design (2x2x3) with 6 replicates. The first factor was the type of trays: i.e., non-perforated and perforated tray. The second factor was the thickness of the substrate: i.e., 1.5 and 3.0 cm, while the third factor was the duration of fermentation: i.e. 5, and 7 days. The variables observed were moisture content, dry matter loss (DML), mannanase and saccharification activities, soluble protein content, their specific activities, and yield. Statistical analysis showed no interactions between the three factors, but there were interactions between types of trays and substrate thicknesses, as well as type of trays and incubation times on the mannanase activity and yield of mannanase. The results showed that DML was observed on day 7 were around 31.43- 36.89. The highest mannanase activity was observed on the non-perforated tray with 3 cm thickness on day 7. The saccharification activity towards palm kernel meal was better in the non-perforated tray on day 7 but not influenced by The yield value of mannanase and saccharification activities on a non-perforated tray with 3.0 cm thickness on day 7 was also the highest. Based on energy efficiency and the cost of production, it can be concluded that the optimum condition to produce the BS4 enzyme was observed in the non-perforated tray with 3 cm thickness and fermented for 7 days.


mannanase, solid substrate fermentation, Eupenicillium javanicum BS4, thickness, perforated tray

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Abd-Aziz S, Ab-Razak NA, Musa MA, Hassan MA. 2009. Production of mannan-degrading enzymes from Aspergillus niger and Sclerotium rolfsii using palm kernel cake as carbon source. Research Journal of Environmental Sciences. 3 251-256.

Chang CW, Webb C. 2017. Production of a generic microbial feedstock for lignocellulose biorefineries through sequential bioprocessing. Bioresour Technol. 227:35-43. doi: 10.1016/j.biortech.2016.12.055

Jaelani A. 2007. Optimalisasi fermentasi bungkil inti sawit (Elaeis guineensis Jacq) oleh kapang Trichoderma reesei.J Ilmu Ternak. 7:87-94.

Ketaren PP, Sinurat AP, Zainuddin D, Purwadaria T, Kompiang IP. 1999. Bungkil inti sawit dan produk fermentasinya sebagai pakan ayam pedaging. JITV. 4:107-112.

Mairizal. 2013. Pengaruh penggantian sebagian ransum komersil dengan bungkil kelapa hasil fermentasi dengan effective microorganism-4 (em-4) terhadap bobot karkas ayam pedaging. J Petern Indon. 15: 46-51.

Mathew AS, Wang J, Luo J, Yau ST. 2015. Enhanced ethanol production via electrostatically accelerated fermentation of glucose using Saccharomyces cerevisiae. Science Report 5: 15713. doi: 10.1038/srep15713.

Maximilian P. 2017. Produksi mananase dengan fermentasi substrat menggunakan Eupenicillium javanicum BS4 pada bioreaktor baki dengan berbagai ketebalan substrat [Skripsi]. Jakarta: Atma Jaya.

Purwadaria T, Haryati T, Frederick E, Tangendjaja B. 2003a. Optimation of β-mannanase production on submerged culture of Eupenicillium javanicum as well as pH and temperature enzyme characterizations. JITV. 8:46-54.

Purwadaria T, Nirwana N, Ketaren PP, Pradono DI, Widyastuti Y. 2003b. Synergistic activity of enzymes produced by Eupenicillium javanicum and Aspergillus niger NRRL 337 on palm oil factory wastes. Biotropia. 20:1-10

Raimbault M. 1998. General and microbiological aspects of solid substrate fermentation. Electron J Biotechnol. 1(3):26-27. doi: 10.2225/vol1-issue3-fulltext-9.

Rashid SA, Ibrahim D, Omar IC. 2012. Mannanase production by Aspergillus nigerUSM F4 via solid substrate fermentation in a shallow tray using palm kernel cake as a substrate. Mal J Microbiol. 8:273-279. doi:10.21161/mjm.42812.

Sae Lee N. 2007. The Production of fungal mannanase, cellulase and xylanase using palm kernel meal as a substrate. Walailak J Sci Tech. 4(1): 67-82.

Stark JM, Firestone MK. 1995.Mechanisms for soil moisture effects on activityof nitrifying bacteria. Appl Environ Microbiol. 61:218-221.

Shimizu M, Kaneko Y, Ishihara S, Mochizuki M, Sakai K, et al. 2015. Novel β-1,4-mannanase belonging to a new glycoside hydrolase family in Aspergillus nidulans. J Biol Chem. 290: 27914–27927. doi: 10.1074/jbc.M115.661645

Sigres DP, Sutrisno A. 2015. Enzim mananase dan aplikasi di bidang industri : kajian pustaka. J Pangan Agroind. 3:899-908.


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