Identification of GH|MspI and GHR|AluI Gene Polymorphism and Its Association with Calf Birth Weight in Ongole-Grade Cattle at Beef Cattle Research Station

Hartati Hartati

Abstract

Calf birth weight is one of important selection criteria for beef cattle to predict mature body weight and selection for calving ease. The GH and GHR gene are considered candidate genes responsible for growth traits in cattle. The objectives of this study were to identify the polymorphism of GH|MspI and GHR|AluI genes and its association with CBW in Grati-OG cattle. Data records of the calves birth weight were collected from 186 Grati-OG cattle raised by IBCRS from May to December 2017. Genomic DNA were isolated from whole blood and used in genotyping analysis using PCR-RFLP method. The result showed that the average of CBW of Grati-OG cattle in present study was 25.58±3.31 kg. No statistical different for CBW between male and female. The genotype frequency of MspI+/+, MspI+/- and MspI-/- of GH gene were 1.1, 18.8 and 80.1 %, respectively and allele frequency of MspI+ and MspI-/- of GH gene were 0.105 and 0.895, respectively. While the genotype frequency of AA, AG and GG of GHR gene were 66.1, 25.3 and 8.6 %, respectively, and allele frequency of A and G of GHR gene were 0.788 and 0.212, respectively. Although in present study showed that the MspI-/- genotype of GH|MspI and GG genotype of GHR|AluI gene tend to be higher on CBW compared to other genotypes but not statistically significant in Grati-OG cattle. In conclusions, both GH|MspI and GHR|AluI gene are polymorphic but not significantly associated with CBW in Grati-OG cattle.

Keywords

Growth gene, Grati-OG Cattle, calf birth weight

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