Plant extract activities as antioxidant and antibiofilm against chicken gut bacteria

Erika Gracia, S. Magdalena, Elizabeth Wina, Arnold P. Sinurat, Tresnawati Purwadaria


The occurrence of microbial resistance against antibiotic due to the subtherapeutic dosage of antibiotic growth promoter (AGP) in poultry can be prevented by the antibiofilm substance. Plant secondary compounds have some activities like antioxidant, antimicrobial, and antibiofilm. This research was conducted to obtain the plant with the highest activity of antibiofilm and also antioxidant by analyzing several plant secondary compounds as antioxidant and antibiofilm against chicken’s gut bacteria. The tested plants were clove leaves, leaffruit plants, mangosteen peel, cashew nut shell, guava leaves, and bay leaves. These plants were extracted with methanol or n-hexane using sonication method. The antioxidant activity as the IC50 value of the plant methanol extracts were determined using α,α-diphenyl-β-picrylhydrazyl (DPPH) assay. The biofilm inhibition activity was tested against Escherichia coli, Salmonella enteritidis, and Staphylococcus aureus ATCC® 29213TM using methanol and n-hexane extracts. All of the samples had antioxidant activity. The clove leaves and leaffruit plants had the highest antioxidant activity, while mangosteen peel extract in methanol had the highest antibiofilm activity against all tested bacteria. The species of bacteria also affected the antibiofilm activity. E. coli and S. enteritidis were more resistant to antibiofilm then S. aureus. Mangosteen peel extract which showed high antioxidant and antibiofilm activity is potential to be used as a feed additive to control the pathogenic bacteria.


Chicken; Plant; Secondary Compounds; Antioxidant; Antibiofilm

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Annegowda HV, Anwar LN, Mordi MN, Ramanathan S, Mansor SM. 2010. Influence of sonication on the phenolic content and antioxidant activity of Terminalia catappa L. leaves. Pharmacognosy Res. 2:368–373.

Aparna Y, Kranthi SU, Sarada J. 2014. Syzygium aromaticum – a common food spice with potential quorum quenching activity on Serratia sps YAJS. Int J Curr Microbiol Appl Sci. 3:93–102.

Biswas B, Rogers K, McLaughlin F, Daniels D, Yadav A. 2013. Antimicrobial activities of leaf extracts of guava (Psidium guajava L.) on two gram-negative and gram-positive bacteria. Int J Microbiol. 2013:1–7.

Bjarnsholt T, Ciofu O, Molin S, Givskov M, Høiby N. 2013. Applying insights from biofilm biology to drug development — can a new approach be developed? Nat Rev Drug Discov. 12:791–808.

Budzyńska A, Wieckowska-Szakiel M, Sadowska B, Kalemba D, Rózalska B. 2011. Antibiofilm activity of selected plant essential oils and their major components. Polish J Microbiol. 60:35–41.

Djordjevic D, Wiedmann M, McLandsborough LA. 2002. Microtiter plate assay for assessment of Listeria monocytogenes biofilm formation. Appl Environ Microbiol. 68:2950–2958. doi: 10.1128/AEM.68.6. 2950-2958.2002.

Er B, Demirhan B, Onurdağ FK, Özgacar SÖ, Öktem AB. 2014. Antimicrobial and antibiofilm effects of selected food preservatives against Salmonella spp. isolated from chicken samples. Poult Sci. 93:695–701.

Geethashri A, Manikandan R, Ravishankar B, Shetty AV. 2014. Comparative evaluation of biofilm suppression by plant extracts on oral pathogenic bacteria. J Appl Pharm Sci. 4:20–23.

Harlita, Satuti NHN, Sagi M, Astuti P. 2016. Acute toxicity of cashew nut shell extract (anacardium occidentale l.) in albino rat (Rattus norvegicus Berkenhout 1769). Pakistan J Biol Sci. 19:89–94.

Høiby N, Bjarnsholt T, Givskov M, Molin S, Ciofu O. 2010. Antibiotic resistance of bacterial biofilms. Int J Antimicrob Agents. 35:322–332. doi: 10.1016/ j.ijantimicag.2009.12.011.

Koh CL, Sam CK, Yin WF, Tan L, Krishnan T, Chong Y, Chan KG. 2013. Plant-Derived Natural Products as Sources of Anti-Quorum Sensing Compounds. Sensors. 13:6217–6228.

Kusuma IW, Kuspradini H, Arung ET, Aryani F, Min YH, Kim JS, Kim YU. 2011. Biological Activity and Phytochemical Analysis of Three Indonesian Medicinal Plants, Murraya koenigii, Syzygium polyanthum and Zingiber purpurea. J Acupunct Meridian Stud. 4:75–79.

Lazar V, Colta T, Marutescu L, Ditu LM, Chifiriuc MC. 2013. New antiinfectious strategy based on antimicrobial and quorum sensing inhibitors from vegetal extracts and propolis. In: Méndez-Vilas A, editor. Microb Pathog Strateg Combat Them Sci Technol Educ Badajoz. Babajos (Spain): Formatex Research Center; p. 1209–1219.

Lin J. 2014. Antibiotic growth promoters enhance animal production by targeting intestinal bile salt hydrolase and its producers. Front Microbiol. 5:1–4. doi: 10.3389/ fmicb.2014.00033.

Lin J, Hunkapiller AA, Layton AC, Chang YJ, Robbins KR. 2013. Response of intestinal microbiota to antibiotic growth promoters in chickens. Foodborne Pathog Dis. 10:331–337.

Nguyen PTM, Vo BH, Tran NT, Van QD. 2015. Anti-biofilm activity of α-mangostin isolated from Garcinia mangostana L. Zeitschrift für Naturforsch C. 70:313–318.

Palakawong C, Sophanodora P, Pisuchpen S, Phongpaichit S. 2010. Antioxidant and antimicrobial activities of crude extracts from mangosteen (Garcinia mangostana L.) parts and some essential oils. Int Food Res J. 17:583–589.

del Pozo JL, Patel R. 2007. The Challenge of Treating Biofilm-associated Bacterial Infections. Clin Pharmacol Ther. 82:204–209.

Rodrigues CJC. 2014. The importance of lipids to biofilm formation: [Lisbon (PRT)]: Instituto Superior Técnico.

Sarin B, Verma N, Martín JP, Mohanty A. 2014. An Overview of important ethnomedicinal herbs of phyllanthus species: Present Status and Future Prospects. Sci World J. 2014:1–12. doi: 10.1155/2014/ 839172.

Selim SA, Adam ME, Hassan SM, Albalawi AR. 2014. Chemical composition, antimicrobial and antibiofilm activity of the essential oil and methanol extract of the Mediterranean cypress (Cupressus sempervirens L.). BMC Complement Altern Med. 14:179.

Shekhar TC, Anju G. 2014. Antioxidant activity by DPPH radical scavenging method of Ageratum conyzoides Linn. leaves. Am J Ethnomedicine. 1:244–249.

Sinurat AP, Wina E, Rakhmani S, Wardhani T, Haryati T, Purwadaria T. 2017. Bioactive substances of some herbals and their effectiveness as antioxidant, antibacteria and antifungi. JITV 23.

Skyberg JA, Siek KE, Doetkott C, Nolan LK. 2007. Biofilm formation by avian Escherichia coli in relation to media, source and phylogeny. J Appl Microbiol. 102:548–554. doi: 10.1111/j.1365-2672.2006.03076.x.

Sultana B, Anwar F, Ashraf M. 2009. Effect of extraction solvent/technique on the antioxidant activity of selected medicinal plant extracts. Molecules. 14:2167–2180.

Taiwo ElA. 2015. Cashew nut shell oil — a renewable and reliable petrochemical feedstock. In: Patel V, editor. Adv Petrochemicals: InTech.

Xie J, Schaich KM. 2014. Re-evaluation of the 2,2-Diphenyl-1-picrylhydrazyl free radical (DPPH) assay for antioxidant activity. J Agric Food Chem. 62:4251–4260.


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