Aflatoxin M1 in fresh dairy milk from small individual farms in Indonesie

Raphaella Widiastuti

Abstract

This present study was aimed to investigate the presence of aflatoxin M1 (AFM1) residue in fresh dairy milk collected from small individual farms. A total of 104 samples of fresh cow's milk were collected from 3 different locations namely Pengalengan-Bandung and Sukabumi (West Java province), and Tanggamus (Lampung province) in April and September 2012. All samples were analyzed using a high performance liquid chromatography (HPLC) and detected with fluorescence detector after extraction with organic solvent. The results showed that high incidence of AFM1 contamination was found in samples from Pangalengan-Bandung and Sukabumi collected in September 2012 with the highest concentration of 34.1 ng/L in a sample from Pangalengan-Bandung, but not found in samples from Lampung for sampling time neither in April and September 2012 Aflatoxin B1 (AFB1) contaminants in low levels were also found in various feed samples. In this study, no sample containing AFM1 exceeded the maximum residue limit (MRL) regulated by the Indonesian government (500 ng/L). Although the results obtained in this study are still not harmful to human health, but the presence of AFM1 in milk and its products should be monitored continuously given the milk is one type of food source that is consumed mainly by children.

Keywords

residue, aflatoxin M1, milk, small individual farms, HPLC

References

Agus A, Khuluq I, Sumantri I, Noviandi CT, Nuryono. 2010. Aflatoxin M1 excretion in the milk of tropical dairy cow fed contaminated aflatoxin B1 in the diet. The 5th International Seminar on Tropical Animal Production Community Empowerment and Tropical Animal Industry October 19-22, 2010, Yogyakarta, Indonesia. 282-285.

Agus A, Sumantri I, Murti TW, Boehm J. 2013. Survey on the occurence of aflatoxin b1 contamination in dairy ration and its carry over into the milk in Yogyakarta and Central Java Provinces of Indonesia. Paper for Presented in ISM-Mycored International Conference Europe 2013 Apulia, Italy 27-31 May 2013.

Anukul N,Vangnai K, Mahakarnchanakul. 2013. Significance of regulation limits in mycotoxin contamination in Asia and risk management programs at the national level. J. Food and Drug Analysis. 21: 227-241

Asi MR, Iqbal SZ, Arino A, Hussain A.2012. Effect of seasonal variations and lactation times on aflatoxin M1 contaminationin milk of different species from Punjab, Pakistan. Food Control 25 : 34-38

[BSN] Badan Standardisasi Nasional. 2009. SNI 7385:2009. Batas maksimum mikotoksin dalam pangan.

[BPOM] Badan Pengawas Obat dan Makanan Republik Indonesia, 2009. Peraturan Kepala Badan Pengawas Obat dan Makanan Republik Indonesia. Nomor HK.00.06.1.52.4011 tentang Penetapan Batas Maksimum Cemaran Mikroba dan Kimia dalam Makanan.

[BSN] Badan Standardisasi Nasional.. 2009. SNI. 3148.1:2009. Pakan konsentrat – Bagian I : Sapi Perah. Badan Standardisasi Nasional,

Codex Allimentarius. 2015. General Standard for Contaminants and Toxins in Food and Feed (Codex STAN 193-1995). Downloaded from www.fao.org/input/download/.../17/CXS_193e

_2015.pdf on 28 August 2018.

Creppy EE. 2002. Update of survey, regulation and toxic effects of mycotoxins in Europe. Toxicology Letters, 127(1-3), 19-28.

van Egmond HP. Mycotoxins in Dairy Products. Elsevier Applied Science; London, UK: 1989. Aflatoxin M1: Occurrence, Toxicity, Regulation; pp. 11–55.

Fernandes AM, Corrêa B, Rosim RE, Kobashigawa E, Oliveira CAF. 2012. Distribution and stability of aflatoxin M1 during processing and storage of Minas Frescal cheese. Food Control. 24 (1-2) : 104-108

Fillaeli A. 2007. Cemaran alami aflatoksin B1 dalam pakan konsentrat dan aflatoksin M1 dalam susu sapi perah. Thesis. Gadjah Mada University, Indonesia.

Food and Agricultural Organization/World Health Organization (FAO/WHO). (1992). Standards

programme. Codex Alimentarius Commission, 1992. Alinorm 93/12. Rome

FAO 2004. Worldwide regulations for mycotoxins in food and feed in 2003. Fppd and Nutrition Paper 81. Rome (Italy) : Food and Agriculture Organization of the United Nations.

Goncalves L, Dalla Rosa A, Gonzales SL, Feltes MC, Baliale-Furlong E, Dors GC. 2017. Incidence of aflatoxin M1 in fresh milk from small farms. Food Sci. Technol, Campinas, 37 (Special issue) : 11-15

Kang’Ethe EK, Lang’AKA. 2009. Aflatoxin B1 and M1 contamination of animal feeds and milk from urban centers in Kenya. African Health Sciences. 9(4), 218-226.

Kılıç Altun S, Gürbüz S, Ayağ E. 2017. Aflatoxin M1 in human breast milk in southeastern Turkey. Mycotoxin Res. 33(2):103-107.

Mahmoudi R, Norian R. 2015. Aflatoxin B1 and M1 contamination in cow feeds and milk from Iran. Food and Agricultural Immunology. 26( 1) :131–137,

Nadira AF, Rosita J, .Norhaizan ME, Redzwan SM.2017. Screening of aflatoxin M1 occurrence in selected milk and dairy products in Terengganu, Malaysia. Food Control 73 (part B) : 209-214

Nuryono N, Agus A,Wedhastri S, Maryudani YB, Setyabudi FMCS, Bohm J, Razzazi-Fazeli E. 2009. A limited survey of aflatoxin M1 in milk from Indonesia by ELISA. Food Control 20 : 721–724

Nurhayati D. 2014. Deteksi aflatoksin M1 pada susu pasteurisasi. Thesis of Bogor Agricultural University (IPB), Indonesia.

Ostry V, Malir F, Toman J, Grosse Y. 2017. Mycotoxins as humans carcinogens-The IARC Monograph classification. Mycotoxin Res. 33:63-75.

Prandini A, Tansini G, Sigolo S, Filippi L, Laporta M, Piva G. 2009. On the occurrence of aflatoxin M1 in milk and dairy products. Food Chem Toxicol. 47(5):984-91.

Picinin LCA, Cerqueira MMOP, Vargas EA, LanaÂMOQ, ToaldoI M, Bordignon-Luiz MT. 2013. Influence of climate conditions on aflatoxin M1 contamination in raw milk from Minas Gerais State, Brazil. Food Control 31 : 419-424.

Ruangwises N, Ruangwises S, 2010. Aflatoxin M1 Contamination in Raw Milk within the Central Region of Thailand. Bull. Environ. Contam. Toxicol. 85(2):195-198

Șanlı T,Deveci O, Sezgİn E. 2012. Effects of pasteurization and storage on stability of aflatoxin M1 in yogurt. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 18 (6) : 987-990.

Shrivastava A, Gupta VB, 2011 Methods for the determination of limit of detection and limit of quantitation of the analytical methods. Chronicles of Young Scientists. 2 (1) : 21-25. 

Shuib NS, Makahleh A, Salhimi SM, Saad B. 2017. Natural occurrence of aflatoxin M1 in fresh cow milk an human milk. Food Control, 73: 966-970.

Sumantri I, Murti TW, Van der Poel AFB, Boehm J, Agus A. 2012. Carry-over of aflatoxin B1-feed into aflatoxin M1-milk in dairy cows treated with natural sources of aflatoxin and bentonite. J.Indonesian Trop.Anim. Agric. 37(4) : 271-277.

Widiastuti R, Maryam R, Bahri S. 2006. Aflatoxin M1 (AFM1) in fresh dairy milk in Pangalengan and Bogor Districts, West Java. Proc. Seminar Nasional Teknologi Peternakan dan Veteriner. Bogor, 5-6 September 2006. Eds. Mathius IW, Sendow I, Nurhayati, Murdiati TB, Thalib A, Beriajaya, Suparyanto A, Prasetyo LH, Darmono, Wina E. Pusat Penelitian dan Pengembangan Peternakan, p. 239 – 243.

Widiastuti R, Indraningsih, Firmansyah R. 2009. Analysis of aflatoxin in corn which purified with SPE silica and detected with HPLC Proc. Seminar Nasional Teknologi Peternakan dan Veteriner 2008, Bogor 11-12 November 2008. Eds. Sani Y, Martindah E, Nurhayati, Puastuti W, Sartika T, Parede L, Anggraeni A, Natalia L. Pusat Penelitian dan Pengembangan Peternakan, 705-710.

Wijaya HR, Widjajanti NI, Silitonga RF. 2018. Detection of aflatoxin M1 in powdered milk and sweetened condensed milk products in several cities in Java with HPLC-fluorescence method. International Symposium on Food and Agro-biodiversity (ISFA) 2017. IOP Conf. Series: Earth and Environmental Science 102 (2017) 012028

Refbacks

  • There are currently no refbacks.

Copyright (c)  2018 Indonesian Journal of Animal and Veterinary Sciences

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.