Supplementation of molasses and branched-chain amino acid to increase in vitro digestibility of ammoniated corn cob in ruminants feed

Wisri Puastuti, Dwi Yulistiani, Eko Handiwirawan


Corn cob contains high fiber and lignin which causes low nutritive value. The objective of the study was to improve the digestibility of ammoniated corn cob (CC) by supplementation of molasses and branched-chain amino acid (BCAA: valine, leucine and isoleucine). CC was processed by addition 3% urea. The first stage of in vitro test was done with 4 levels of molases 0, 5, 10 and 15% of dry matter (DM) of CC. The experiment was carried out using complete randomized design with 4 treatments and 4 replications. The second stage was also in vitro study of supplementation of two levels each for valine = V, leucine = L and isoleucine = I namely 0.1 and 0.2% of DM of ammoniated CC. There were 8 treatments combination of the BCAA as follow: A = V0.1 L0.1 I0.1; E = V0.2 L0.1 I0.1; B = V0.1 L0.1 I0.2; F = V0.2 L0.1 I0.2; C = V0.1 L0.2 I0.1; G = V0.2 L0.2 I0.1; D = V0.1 L0.2 I0.2; H = V0.2 L0.2 I0.2 and 1 control (V0.0 L0.0 I0.0). The experiments were done using completely randomized design with 9 treatments and 3 replications. The results showed that treated CC with urea was able to increase protein content by 78% (increased from 3.34% to 5.95%) while neutral detergent fiber (NDF) decreased by 15.4%, acid detergent fiber (ADF) by 7.9% and lignin 16.7%. Addition of molasses in ammoniated CC increased DM digestibility (P <0.05) by 7.5% (41.9 vs 43.51-46.26%) and NDF by 17.7% (38.41 vs 43.76 – 46.31%). Supplementation of BCAA resulted in the highest DM, OM and NDF digestibility (P <0.05) in the treatment of A, C, D and G. Compare to treament I, the digestibility of DM, OM and NDF in G treatment increased by 31.4%, 27.5% and 36.5%, respectively and produced the highest total population of rumen bacterial of 12.4 x 109 colonies /ml. It can be concluded that the digestibility of ammoniated CC increased by the supplementation of 5% molases and BCAA combination consisted of 0.2% valine, 0.2% leucine and 0.1% isoleucine.


Kata kunci: Tongkol jagung, amoniasi, molases, asam amino bercabang.


Corn Cob; Ammoniation; Molasses; Branched-Chain Amino Acid

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Adeniji AA. 2010. Effects of dietary grit inclusion on the utilization of rice rusk by pullet chicks. Trop Subtrop Agroecosyst. 12:175-180.

Al Qori’ah, Surono, Sutrisno. 2016. Sintesis protein mikroba dan aktivitas selulolitik akibat penambahan level zeolit sumber nitrogen slow release pada glukosa murni secara in vitro. Jurnal Ilmu-Ilmu Peternakan. 26:1-7.

Alam MK, Ogata Y, Sato Y, Sano H. 2016. Effects of rice straw supplemented with urea and molasses on intermediary metabolism of plasma glucose and leucine in sheep. Asian Australas J Anim Sci. 29:523-529.

[AOAC] Association of Official Analytical Chemist. 1990. Association of Official Analytical Chemist, Official Method of Analysis. 12th ed. AOAC.

Bach A, Calsamiglia S, Stern MD. 2005. Nitrogen metabolism in the rumen. J Dairy Sci. 88:E9‐E21.

Bata M. 2008. Pengaruh molases pada amoniasi jerami padi menggunakan urea terhadap kecernaan bahan kering dan bahan organik in vitro. Agripet. 8:15-20.

Blummel M, Steingass H, Becker K. 1997. The relationship between in vitro gas production, in vitromicrobial biomass yield and 15N incorporation and itsimplications for the prediction of voluntary feed intake of roughages. Br J Nutr. 77:911-921.

Chumpawadee S, Sommart K, Vongpralub T, Pattarajinda V. 2006. Effects of synchronizing the rate of dietary energy and nitrogen release on ruminal fermentation, nitrogen and nutrient digestibility in beef cattle. Asian-Australas J Anim Sci. 19:181-188.

Clark JH, Klusmeyer TH, Cameron MR. 1992. Microbial protein synthesis and flows of nitrogen fractions to the duodenum of dairy cows. J Dairy Sci. 75:2304-2323.

Dean DB, Adesogan AT, Krueger NA, Little RC. 2008. Effect of treatment with ammonia or fibrolytic enzymes on chemical composition and ruminal degradability of hays produces from tropical grasses. Anim Feed Sci Technol. 145:68-83.

Febrina D. 2012. Kecernaan ransum sapi Peranakan Ongole berbasis limbah perkebunan kelapa sawit yang diamoniasi urea. Jurnal Peternakan. 9:68-74.

Gunun P, Wanapat M, Anantasook N. 2013. Effects of physical form and urea treatment of rice straw on rumen fermentation, microbial protein synthesis and nutrient digestibility in dairy steers. Asian-Australas J Anim Sci. 26:1689-1697.

Hartadi H, Reksohadiprodjo S, Tillman AD. 1997. Tabel komposisi pakan untuk Indonesia. Cetakan Kedua. Yogyakarta (Indones): Gadjah Mada University Press.

Hastuti D, Shofia Nur A, Iskandar Muda B. 2011. Pengaruh perlakuan teknologi amofer (amoniasi fermentasi) pada limbah tongkol jagung sebagai alternatif pakan berkualitas ternak ruminansia. Mediagro. 7:55-65.

Hailu A, Melaku S, Tamir B, Tassew A. 2011. Body weight and carcass characteristics of Washera sheep fed urea treated rice straw supplemented with graded levels of concentrate mix. Livest Res Rural Develop. Vol. 23

Article 164.

Huyen NT, Wanapat M, Navanukraw C. 2012. Effect of mulberry leaf pellet (MUP) supplementation on rumen fermentation and nutrient digestibility in beef cattle fed on rice straw-based diets. Anim Feed Sci Technol. 175:8-15.

Khejornsart P, Wanapat M. 2010. Effect of chemical treatment of rice straw on rumen fermentation characteristic, anaerobic fungal diversity in vitro. J

Anim Vet Adv. 9:3070-3076.

Laconi EB, Jayanegara A. 2015. Improving nutritional quality of cocoa pod (Theobroma cacao) through chemical and biological treatments for ruminant feeding: In vitro and in vivo evaluation. Asian-Australas J Anim Sci.


Mapato C, Wanapat M, Cherdthong A. 2010. Effect of urea treatment of straw and dietary level of vegetable oil on lactating dairy cows. Trop Anim Health Prod. 42:1635-1642.

McAllen AB, Smith RH. 1983. Factor influencing the digestion of dietary carbohydrat between the mouth and abomasum of steers. Br J Nutr. 50:445.

Menke KH, Steingass H. 1988. Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid. Anim Res Dev. 28:7-55.

Moharrery A. 2004. Effect of isoacids on some rumen enzymes. J Anim Feed Sci. 13:159-162.

Mustofa Z, Tampoebolon BIM; Subrata A. 2012. Peningkatan kualitas tongkol jagung teramoniasi melalui teknologi fermentasi menggunakan starter komersial terhadap produksi VFA dan NH3 rumen secara in vitro. Anim Agric J. 1:599-609.

Nurfeta A, Tolera A, Eik LO, Sundstol F. 2008. Feeding value of enset (Ensete ventricosum), Desmodium intortum hay and untreated or urea and calsium oxide treated wheat straw for sheep. J Anim Physiol Anim Nutr. 93:94-104.

Nurhaita, Ningrat RWS. 2011. Efek suplementasi daun ubi kayu terhadap kecernaan daun sawit amoniasi secara in vitro. Jurnal Peternakan Indones. 13:43-47. Nurhaita N, Jamarun L, Warly, Zain M. 2010. Kecernaan ransum domba berbasis daun sawit teramoniasi yang disuplementasi sulfur, fosfor, dan daun ubi kayu. Media Peternakan, 2010:144-149.

Nurhaita, Ruswendi, R Wismalinda, Robiyanto. 2014. Optimizing rumen bioprocess through supplementation of microbe precursor nutrient in ammoniation of palm oil frond-base cattle ration. J Adv Agric Technol. 1:10-13.

Ogimoto K, Imai S. 1981. Atlas of rumen microbiology. JSSP. Tokyo.

Oji UI, Etim HE, Okoye FC. 2007. Effects of urea and aqueous ammonia treatment on the composition and nutritive value of maize residues. Small Rumin Res. 69:232-236.

Pathak AK. 2008. Various factors affecting microbial protein synthesis in the rumen. Vet World. 1:186-189.

Prastyawan RM, Tampoebolon BIM, Surono. 2012. Peningkatan kualitas tongkol jagung melalui teknologi amoniasi fermentasi (amofer) terhadap kecernaan bahan kering dan bahan organik serta protein total secara in

vitro. Anim Agric J. 1:611-621.

Puastuti W, Yulistiani D, Mathius IW, Giyai F, Dihansih E. 2010. Ransum berbasis kulit buah kakao yang disuplementasi Zn organik: Respon pertumbuhan pada domba. JITV. 16:269-277.

Puastuti W. 2009. Manipulasi bioproses dalam rumen untuk meningkatkan penggunaan pakan berserat. Wartazoa. 19:180-190.

Ramirez GR, Aguilera-Gonzalez JC, Garcia-Diaz G, Nunez-Gonzalez AM. 2007. Effect of urea treatment on chemical composition and digestion of Cenchrus ciliaris and Cynodon dactylon hays and Zea mays residues. J Anim Vet Adv. 6:1036-1041.

SAS Institute. 2002. The SAS System for Windows. Release Version 9.0. Cary. NC (USA): SAS Inst.

Santoso B, Hariadi BTj, Manik H, Abubakar H. 2009. Kualitas rumput unggul tropika hasil ensilase dengan bakteri asam laktat dari ekstrak rumput terfermentasi. Media Peternakan. 32:137-144.

Satter LD, Slyter LL. 1974. Effect of ammonia concentration of rumen microbial protein production in vitro. Br J Nutr. 32:199-208.

Scholljegerdes EJ, Weston TR, Ludden PA, Hess BW. 2005. Supplementing a ruminally undegradable protein supplement to maintain essential amino acid supply to the small intestine when forage intake is restricted in beef cattle. J Anim Sci. 83:2151‐2161.

Setyadi JH, Tri Rahardjo, Suparwi. 2013. Kecernaan bahan kering dan bahan organik tongkol jagung (Zea mays) yang difermentasi dengan aspergillus niger secara in vitro. Jurnal Ilmiah Peternakan. 1:170-175.

Sumarsih S, Sutrisno CI, Pangestu E. 2007. Kualitas nutrisi dan kecernaan daun eceng gondok amoniasi yang difermentasi dengan Trichoderma viride pada berbagai lama pemeraman secara in vitro. J Indones Trop Anim

Agric. 32:257-261.

Tedeschi LO, Fox DG, Fonseca MA, Francis L, Cavalcanti L. 2015. Models of protein and amino acid requirements for cattle: Invited Riview. R Bras Zootec. 44:109-132.

Ubwa ST, Abah J, Oshidi BA, Otokpa E. 2014. Studies on urea treated rice milling waste and its application as animal feed. Afr J Pure Appl Chem. 8:23-31.

Van Soest PJ. 2006. Rice straw the role of silica and treatment to improve quality. J Anim Feed Sci Technol. 130:137-171.

Van Soest PJ, Robertson JB, Lewis BA. 1991. Methods for dietary fiber, neutral detergent fiber and non-starch polysaccharides in relation to animal nutrition. J Dairy Sci. 74:3583-3593.

Volden H. 1999. Effects of level of feeding and ruminally undegraded protein on ruminal bacterial protein synthesis, escape of dietary protein intestinal amino acid profile, and performance of dairy cows. J Anim Sci. 77:1905‐1918.

Wanapat M, Polyrach S, Boonnop K, Mapato C, Cherdthong A. 2009. Effect of treating rice straw with urea and calcium hydroxide upon intake, digestibility, rumen fermentation and milk yield of dairy cows. Livest Sci. 125:238-243.

Widiawati Y. 2002. The utilisation of shrub legumes: Leucaena leucocephala, Gliricidia sepium and Calliandra callothyrsus for growing sheep (Thesis). Townsville (Australia): James Cook University.

Yadete GK. 2014. Effect wheat straw urea treatment and Leucaena leucochepala foliage hay supplementation on intake, digestibility, nitrogen balance and growth of lambs. Int J Livest Prod. 6:88-96.

Yalchi T, Afzalzade A, Sharifi RS. 2009. Effects of liquid ammonia and urea treatment on chemical composition and in vitro digestibility of triticale straw. J Anim Vet Adv. 8:1916-1920.

Yulistiani D, Puastuti W, Wina E, Supriati. 2012. Pengaruh berbagai pengolahan terhadap nilai nutrisi tongkol jagung: Komposisi kimia dan kecernaan in vitro. JITV. 17:59-66.

Zain M. 2007. Optimalisasi penggunaan serat sawit sebagai pakan serat alternatif dengan suplementasi daun ubi kayu dalam ransum ruminansia. J Indones Trop Anim Agric. 32:100-105.

Zain M, Jamarun N, Nurhaita. 2010. Effect of sulfur suplementation on in vitro fermentability and degradability of ammoniated rice straw. Pak J Nutr.


Zain M, Sutardi T, Suryahadi; Ramli N. 2008. Effect of defaunation and supplementation methionine hydroxy analogue and branched chain amino acid in growing sheep diet based on palm press fiber ammoniated. Pak J Nutr. 7:813-816.

Zhang HL, Chen Y, Xu XL, Yang YX. 2013. Effects of branched-chain amino acids on In vitro ruminal fermentation of wheat straw. Asian-Aust J Anim Sci. 26:523-528.


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