Study of BMP15 gene polymorphism in Boer, Kacang, and Boerka goats

Aron Batubara, Simon Elieser, Cece Sumantri


The bone morphogenetic protein 15 (BMP15) gene or commonly called FecX (fecundity chromosome X) is a gene that controls the prolific properties. This study aims to identify the mutation of BMP15 gene and to analyze its polymorphism in Boer, Kacang, and Boerka goats. The total of 50 female goat bloods were identified using PCR-Sequencing method, 17 Boer, 16 Kacang and 17 Boerka respectively. BMP15 gene amplification resulted fragment with the length of 141 bp. Genotyping of BMP15 gene produced three genotypes. The result showed that BMP15 gene found two polymorphic SNP were analyzed by genotype frequency, allele frequency, heterozygosis and equilibrium of genotype in all population was detected by the Hardy-Weinberg equilibrium test (χ2). Sequence analysis results of BMP15 gene showed that there were two mutation between adenine (A) and guanine (G) bases and determination of genotype BMP15 gene produces three genotypes showed that there were GG, GA and AA. In conclusion, there was found the mutation of BMP15 gene in Boer, Kacang and Boerka goats and genetic polymorphism were identified using PCR-Sequencing method.


BMP15 Gene; PCR-Sequencing; Prolific; Genetic Polymorphism; SNP

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Abdel-Rahman SM, Mustafa YA, Abd Errasool HA, El-Hanafy AA, Elmaghraby AM. 2013. Polymorphism in BPM-15 gene and its association with litter size in Anglo-Nubian goat. Biotechnol Anim Husb. 29:675-683.

Allendorf FW, Luikart GH, Aitken SN. 2013. Conservation and the genetics of populations. 2nd ed. UK: Wiley-Blackwell.

Batubara A, Rr N, Farajallah A, Tiesnamurti B. 2013. Keragaman genetik DNA Y-Kromosom pada enam rumpun kambing lokal Indonesia. Nurhayati D. Purwantari, Muharam Saepulloh, Sofjan Iskandar, Anneke Anggraeni, Simon P. Ginting, Atien Priyanti, Ening Wiedosari, Dwi Yulistiani, Ismeth Inounu, Sjamsul Bahri, Wisri Puastuti. Prosiding Seminar Nasional Teknologi Peternakan dan Vet. 4:316–325.

Bodin A, Ahrenstedt L, Fink H, Brumer H, Risberg B, Gatenholm P. 2007. Modification of nanocellulose with a xyloglucan-rgd conjugate enhances adhesion and proliferation of endothelial cells: Implications for tissue engineering. Biomacromolecules. 8:3697-3704.

Chu MX, Jiao CL, He YQ, Wang JY, Liu ZH, Chen GH. 2007. Association between PCR-SSCP of bone morphogenetic protein 15 gene and prolificacy in Jining Grey Goats. Anim Biotechnol. 18:263-274.

Chu MX, Liu ZH, Jiao CL, He YQ, Fang L, Ye SC, Chen GH, Wang JY. 2007. Mutations in BMPR-IB and BMP-15 genes are associated with litter size in Small Tailed Han sheep (Ovis aries). J Anim Sci. 85:598-603.

Cui H, Ghosh SK, Jayaram M. 2009. The selfish yeast plasmid uses the nuclear motor Kip1p but not Cin8p for its localization and equal segregation. J Cell Biol 185:251-264.

Demars J, Fabre S, Sarry J, Rossetti R, Gilbert H, Persani L, Tosser-klopp G, Mulsant P, Nowak Z, Drobik W, et al. 2013. Genome-wide association studies Identify two novel BMP15 mutations responsible for an atypical hyperprolificacy phenotype in sheep. LoS Genet 9:e1003482. doi: 10.1371/journal.pgen.1003482

Elieser S, Sumadi, GS, Subandriyo. 2012. Kinerja reproduksi induk kambing Boer, Kacang dan Boerka. JITV 17:100-106.

Farhadi P, Soosani J, Adeli K, Alijani V. 2013. The effect of destruction in positioning of Quercus brantii in Zagros forests, Iran. J Biodiversity Environment Sci. 3:49-57.

Feng W, Bachant J, Collingwood D, Raghuraman MK, Brewer BJ. 2009. Centromere replication timing determines different forms of genomic instability in Saccharomyces cerevisiae checkpoint mutants during replication stress. Genetics. 183:1249-1260.

Hall T. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser. 41:95-98.

Haryono, Tiesnamurti B, Setiadi B, Ginting SP, Talib C. 2011. Superior breeds of small ruminant produced by Agricultural Agency of Research and Development (AARD). Simon P. Ginting, Aron Batubara, Rasali Hakim Matondang, Tati Herawati, Rini Damayanti, Umi Adiati, Chalid Talib. Proceeding of Workshop Nasional Diversifikasi Pangan Daging Ruminansia Kecil. Kota (Indones): Indonesian Agency of Agricultural Research and Development. p. 3-16.

Hidayat RA, Depamede SN, Maskur. 2015. Identification of mutation on gene FecX BMP15 and its effects on prolific nature of local goats in West Lombok Regency. J Ilmu Teknol Peternakan Indones. 1:1-7.

Hua GH, Chen SL, Yu JN, Cai KL, Wu CJ, Li QL, Zhang CY, Liang AX, Han L, Geng LY, et al. 2009. Polymorphism of the growth hormone gene and its association with growth traits in Boer goat bucks. Meat Sci. 81:391-395.

Jalbani MA, Kaleri HA, Baloch AH, Bangulzai N, Bugti AG, Ashraf F, Kaleri RR, Jan M, Bugti GA, Khosa AN. 2017. Study of BMP15 gene polymorphisim in Lehri goat breed of Balochistan. J Appl Environment Biol Sci. 7:84-89.

Kaps M, Lamberson WR. 2004. Biostatistics for animal science. UK: CABI Publishing. doi: 10.1111/j.1439-0388.2005.00495.x

Marson A, Jacoby A, Johnson A, Kim L, Gamble C, Chadwick D. 2005. Immediate versus deferred antiepileptic drug treatment for early epilepsy and single seizures: a randomised controlled trial. The Lancet. 365:2007-2013.

Monteagudo LV, Ponz R, Tejedor MT, Lavina A, Sierra I. 2009. A 17 bp deletion in the bone morphogenetic protein 15 (BMP15) gene is associated to increased prolificacy in the Rasa Aragonesa sheep breed. Anim Reprod Sci. 110:139-146.

Muladno. 2002. Teknik rekayasa genetika. Bogor (Indones): Pustaka Wirausaha Muda

Nei M, Kumar S. 2000. Molecular evolution and phylogenetics. New York (USA): Oxford University Press.

Noor RR. 2010. Genetika ternak. Jakarta (Indones): Penebar Swadaya.

Pamungkas FA, Batubara A, Doloksaribu M, Sihite E. 2009. Potensi plasma nutfah kambing lokal Indonesia. Pamungkas FA, editor. Bogor (Indones): IAARD Press..

Pelt-Verkuil EV, Belkum AV, Hays JP. 2008. Principles and technical aspects of PCR amplification. AZ Dordrecht (Netherland): Springer, Dordrecht.

Rout PK, Joshi MB, Mandal A, Laloe D, Singh L, Thangaraj K. 2008. Microsatellite-based phylogeny of Indian domestic goats. BioMeds Central Genet. 9:11.

Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. 2013. MEGA6: Molecular evolutionary genetics analysis version 6.0. Mol Biol Evol. 30:2725–2729.

Wang Y, Yuanxiao L, Nana Z, Zhanbin W, Junyan B. Polymorphism of exon 2 of BMP15 gene and its relationship with litter size of two Chinese goats. Asian-Aust J Anim Sci. 24:905-911.

Weir BS. 1996. Genetic data analysis ii methods for discrete population genetic data. Sunderland, Massachusetts: Sinauer Associates, Inc. Publishers.

Zhang C, Zhang Y, Xu D, Li X, Su J, Yang L. 2009. Genetic and phenotypic parameter estimates for growth traits in Boer goat. Livest Sci. 124:66-71.


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