Clenbuterol Residue in Beef Meat Collected From Several Cities In Java Island, Indonesia

Raphaella Widiastuti, Yessy Anastasia


Clenbuterol (CLB) (see Figure 1) is an illegally animal drug of the β-agonist group that used as a promoter of growth in various farm animals. The presence of CLB residues in livestock products can cause poisoning in humans such as tremor, tachycardia, nausea and dizziness. The purpose of this research is to develop CLB residue detection method on beef detected using high performance liquid chromatography (HPLC) and to know the presence of CLB residue on 74 samples of frozen and fresh meat from several cities in Java. Samples were extracted with acetonitrile and isopropanol, then analyzed chromatographically using RDS ODS C18 column and mixed mobile phases of 50 mM NaH2PO4 and acetonitrile (80:20, v/v) and detected by photodiode array detector at 210 nm wavelength. The recoveries test at spiked concentrations of 2, 5 and 10 ng/g were 103.45, 89.27 and 89.53% for each additional concentrations. The detection limit and the quantitation limit were 0.10 ng/g and 0.31 ng/g, respectively. Analysis of 74 samples showed that CLB residue was detected in 8 samples in a concentration range of 2.40 to 15.06 ng/g and had exceeded the CLB residue maximum limit of 0.2 ng/g. To avoid the risk of the presence of CLB residues, it is necessary to regularly monitor the residue content of livestock food to ensure food safety for consumers.


Clenbuterol; Residue; Beef Meat; HPLC


Associated Press, 2016. Mexico watches out for banned steroid clenbuterol in food. Fox News World. 27 April 2016.

Almazan LAC, Ortiz JAD, Cruz BP, Romero MAA. 2012. Trend in levels of clenbuterol in bovine samples from 2005 to 2010 in Guerrero, Mexico. Rev. Mex. Cienc. Pecu, 3(4): 449-458.

Barbosa J, Cruz C, Martins J, Silva JM, Neves C, Alves C, Ramos F, Da Silveira MIN. 2005. Food poisoning by clenbuterol in Portugal. Food Add.& Contam. 22(6): 563-566

Betancurt SDP, Cordova-Izquierdo A, Uribe A, Michel AM. 2008. Cenbuterol residues in bovine feed and meat. Res. J. Biol. Scie. 3(12): 1444-1445.

Brambilla G, Cenci T, Franconi F, Galarinio R, Macri A, Rondoni F, Strozzi M, Loizzo A. 2000. Clinical and pharmacological profile in a clenbuterol epidemic poisoning of contaminated beef meat in Italy. Tox.Lett. 114(1–3):47–53.

[BPMSPH]. 2015. Laporan Tahunan 2015 Balai Pengujian Mutu dan Sertifikasi Produk Hewan. Direktorat Kesehatan Masyarakat Veteriner, Dirjen Peternakan dan Kesehatan Hewan, Kementerian Pertanian.

Chang L-Y, Chou S-S, D-F. Hwang D-F. 2005. High performance liquid chromatography to determine animal drug clenbuterol in pork, beef and hog liver. J. Food and Drug Analysis. 13(2): 163-167

[CAC] Codex Alimentarius Commission. 2012. CAC/MRL 2-2012. Codex Alimentarius Commission. Maximum Residue Limits for Veterinary Drugs in Foods. July 2012. Updated as at the 35th session of the Codex Alimentarius Commission. Diunduh dari

Elliott C.T., W.J. McCaughey, S.R.H. Crooks , J.D.G. McEvoy & D.G. Kennedy. 1995. Residues of clenbuterol in cattle receiving therapeutic doses: Implications for differentiating between legal and illegal use. Veterinary Quarterly. 17: 100-2

[EC] European Commission. 2010. Commission Regulation (EU) No 37/2010 of December 22th 2009, on pharmacologically active substances and their classification regarding maximum residue limits in foodstuffs of animal origin. OJEU. Available at, http:// 2010_37_en.pdf; 2010 [accessed 12.10.12].;

Groot MJ1, Lasaroms JJ, van Bennekom EO, Van Hende J, Nielen MW. 2013. Possible contamination with clenbuterol from treated veal calves to untreated pen mates. Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 30(6):1063-7.

Hajrulai-Musliu Z, Uzunov R, Stojkovski, Dimitrieska-Stojkovik E, Stojanosvska-Dimziska B, Sekulosvski P. 2013. Determination of clenbuterol in meat samples with ELISA and GC-MS method. Proc. 1st Annual International Interdisciplinary Conference.AIIC 2013.24-26 April, Azores, Portugal.817-824.

He L, Pu C, Yang H, Zhao D and Deng A.-P. 2009. Development of a polyclonal indirect ELISA with sub-ng g-1 sensitivity for the analysis of clenbuterol in milk, animal feed, and liver samples and a small survey of residues in retail animal products. Food Add.Contam. 26(8): 1153-1161.

Lai WH, Fung DYC, Xu Y and Xiong YH. 2008. Screening Procedures for Clenbuterol Residue Determination in Raw Swine Livers Using Lateral-Flow Assay and Enzyme-Linked Immunosorbent Assay. J. Food Prot. 71(4): 865–869.

Lee HB, Sarafin K, Peart TE. 2007. Determination of β-blockers and β2-agonist in sewage by solid phase extraction and liquid chromatography tandem mass spectrometry. J. Chromatography A, 1148:158-167

Li L, Zhang J, Tang C and Zhao Q. 2014. Accumulation of Clenbuterol Residues in the Hair of Chinese Simmental Beef Cattle During and After Treatment . J Anal Toxicol. 38(1): 52-56.

Martinez-Navarro, J. F. 1990. Food poisoning related to consumption of illicit beta-agonist in liver. Lancet, 336, 1311.

Pleadin J, Vulić A, Persi N, Vahcić N. 2010. Clenbuterol residues in pig muscle after repeat administration in a growth-promoting dose. Meat Sci. 86(3):733-7.

Sakai N, Sakai M, Mohamad Haron DE, Yoneda M, Ali Mohd M. 2016. Beta-agonist residues in cattle, chicken and swine livers at the wet market and the environmental impacts of wastewater from livestock farms in Selangor State, Malaysia. Chemosphere. 165:183-190.

Sniegocki T, Zmudzki J, Posyniak A and Semeniuk S. 2003. Gas chromatography-mass spectrometric confirmatory method for the determination of clenbuterol residues in animal urine and liver samples. Bull. Vet. Inst. Pulawy 47: 139-144.

Trejo FM, Leon SV, Medina AS, Tolentino RG. 2013. Application of high performance liquid chromatography-UV detection to quantification of clenbuterol in bovine liver samples. J. Food and Drug Analysis, 21:414-420.

Valladares-carranza, B., Bañuelos-valenzuela, R., Pena-Betancourt, S. D., Velazquez-Ordonez, V., Velazquez-Armenta, Y., & Nava-Ocampo, A. 2014. Illegal Use of Clenbuterol in Cattle Production in México. Health, 6(March), 673–676.

Widiastuti, R and Y. Anastasia. 2015. Ractopamine Residue in Imported Beef Meat in Indonesia. Proc. Animal Livestock and Veterinary Technology.


  • There are currently no refbacks.

Copyright (c)  2018 Indonesian Journal of Animal and Veterinary Sciences

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.