Histological changes of liver tissue and serobiochemical relation in does with pregnancy ketosis

A.F.M. Azmi, A.A.A. Ghani, A.S. Saadan, A. Mokrish, K.S. Lai, M. Zamri-Saad, A.B. Zuki, H.A. Hassim

Abstract

Histological changes of liver in does with pregnancy ketosis were characterized. Twenty pregnant does at day 80 of pregnancy were used for this experiment. A total of 10 does were fed by grass (Napier) and goat concentrate with water ad libitum. Those 10 goats considered as healthy pregnant goat, and another 10 goats showing clinical and subclinical signs of ketosis considered as unhealthy pregnant does. Liver biopsies were performed when clinical signs appeared. Beta-Hydroxybutyrate (BHBA), free fatty acid (FFA), and glucose were dosed. Histological preparation revealed similar incidence and intensity of mild liver steatosis with lower cellular vacuolation in hepatocyte presence in healthy late pregnant does. Almost all of the pregnant does with ketosis state (n=8/10) had large amount of small lipid droplets in almost every hepatocyte over the whole liver acinus with higher number of cellular vacuolation, and related with higher BHBA and FFA levels while low in glucose level.

Keywords

Beta-Hydroxybutyrate; Does; Free Fatty Acid; Liver Biopsies; Pregnancy Ketosis

Full Text:

PDF

References

Andrews A. 1997. Pregnancy toxaemia in the ewe. In Pract. 19:306-314.

Bani-Ismail ZA, Al-Majali AM, Amireh F, Al-Rawashdeh OF. 2009. Metabolic profiles in goat do in late pregnancy with and without subclinical pregnancy toxemia. Vet Clin Pathol. 37:434-437.

Bisant K. 2010. Consumer preference for goat meat in Malaysia: Market opportunities and potential. J Agribus Mark. 3:40-55.

Brounts S, Hawkins J, Baird AN, Glickman L. 2004. Outcome and subsequent fertility of sheep and goats undergoing caesarean section because of dystocia: 110 cases (1981–2001). J Am Vet Med Assoc. 224:275-279.

Brozos C, Mavrogianni VS, Fthenakis GC. 2011. Treatment and control of peri-parturient metabolic diseases: Pregnancy toxemia, hypocalcemia, hypomagnesemia. Vet Clin North Am Food Anim Pr. 27:105-113.

Cal L, Borteiro C, Benech A, Rodas E, Abreu MN, Cruz JR, Montana G. 2009. Histological changes of the liver and metabolic correlates in ewes with pregnancy toxemia. Arq Bras Med Vet Zootech. 61:306-312.

Herdt TH. 2000. Ruminant adaptation to negative energy balance. Vet Clin North Am Food Anim Pr. 16:215-230.

Jaeschke H, Gores GJ, Cederbaum AI. 2002. Mechanisms of hepatotoxicity. Toxicol Sci. 65:166-176.

Jamaludin AA, Idris K, Roslaini R. 2012. Challenges facing dairy goat farmers in Malaysia. In: Abdullah R, Omar MA, Makkar H, Otte J, Rajion MA, Alimon AR, Boo LJ, Kam HA, Li CW, editors. Proceeding First Asia Dairy Goat Conference. Kuala Lumpur (Malays): Universiti Putra Malaysia and The Food and Agricultural Organization of the United Nations. p. 11-13.

Jeffrey M, Higgins RJ. 1992. Brain lesions of naturally occurring pregnancy toxaemia of sheep. Vet Pathol. 29:301-307.

Marteniuk JV, Herdt TH. 1988. Pregnancy toxemia and Ketosis of ewes and does. Vet Clin North Am Food Anim Pr. 4:307-315.

Mitchell GM, Stratford BF. 1987. The morphology of the ovine placenta in pregnancy toxaemia. Aust Vet J. 64:221-223.

Noble RC, Steel W, Moore JH. 1971. The plasma lipids of the ewe during pregnancy and lactation. Res Vet Sci. 12:47-53.

Pethick DW, Lindsay DB. 1982. Metabolism of ketone bodies in pregnant sheep. Br J Nutr. 48:549-563.

Rook JS. 2000. Pregnancy toxemia of ewes, does, and beef cows. Vet Clin North Am Food Anim Pr. 16:293-317.

Russel AJF, Donery JN, Reid RL. 1976. The use of biochemical parameters in controlling nutritional state in pregnant ewes and the effect of undernourishment during pregnancy on lamb birth-weight. J Agric Sci Camb. 68:359.

Sadjadian R, Seifi HA, Mohri M, Naseian AA, Farzaneh N. 2013. Variations of energy biochemical metabolites in periparturient dairy Saanen goats. Comp Clin Pathol. 22:449-456.

Sargison ND, Scott PR, Penny CD, Pirie RS, Kelly JM. 1994. Plasma enzmes and metabolites as potential prognostic indices of ovine pregnancy toxaemia a preliminary study. Br Vet J. 150:271.

Schlumbohm C, Harmeyer J. 2004. Hyperketonemia impairs glucose metabolism in pregnant ewes. J Dairy Sci. 87:350-358.

Snook LC. 1939. Fatty infiltration of the liver in pregnant ewes. J Physiol. 97:238-249.

Stockhaus C, Van Den Ingh T, Rothuizen J. 2004. A multistep approach in the cytologic evaluation of liver biopsy samples of dogs with hepatic diseases. Vet Pathol. 41:461-470.

Terashima Y, Tucker RE, Deets LE, Degregorio RM, Muntifering RB, Mitchell CE. 1982. Plasma magnesium levels as influenced by cold exposure in fed or fasted sheep. J Nutr. 112:1914-1920.

Zamir S, Rozov A, Gootwine E. 2009. Treatment of pregnancy toxaemia in sheep with flunixin meglumine. Vet Rec. 165:265-266.

Refbacks

  • There are currently no refbacks.

Copyright (c)  2016 Indonesian Journal of Animal and Veterinary Sciences

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.