Gas production and rumen fermentation characteristics of buffalo diets containing by-product from some sorghum varieties

Irawan Sugoro, Komang G. Wiryawan, Dewi Apri Astuti, Teguh Wahyono

Abstract

Sorghum is one of potencial fibre sources as buffalo feed. Quality of sorghum could be increased by irradiation mutation breeding. Samurai 1 and Samurai 2 were products of the irradiation mutation breeding of Pahat. This study was conducted to compare buffalo diets containing Samurai 2 sorghum straw and Samurai 1 bagasse sorghum compared with Pahat sorghum straw using in vitro study. Completely randomized design with 6 treatments and 3 replications was applied in this experiment. The treatment diets were P1 (50% Pahat sorghum straw + 50% concentrate), P2 (50% Pahat sorghum straw silage + 50% concentrate), P3 (50% Samurai 2 sorghum straw + 50% concentrate), P4 (50% Samurai 2 sorghum straw silage + 50% concentrate), P5 (50% Samurai 1 sorghum bagasse + 50% concentrate) and P6 (50% Samurai 1 sorghum bagasse silage + 50% concentrate). The 200 mg DM samples of diets were incubated in 30 ml rumen-buffer fluid for 48 hours. Variables measured were total gas production, CH4 production and rumen fermentation characteristics. Results showed that P2 and P4 produce the highest of gas production (P<0.05) with 60.99 and 60.86 ml/200 mg dry matter respectively. Treatments of P1, P2 and P4 produced the lowest CH4 concentration (P<0.05) with 10.57, 10.90 and 9.82% of total gas, respectively. The P4 produced the highest total volatile fatty acids (VFA), dry matter degradability and organic matter degradability with 109.83 mM, 62.93% and 59.97% respectively, meanwhile ammonia (NH3) concentration was not significantly different. The conclusion showed that straw silage of Samurai 2 was comparable to the Pahat sorghum straw and Samurai 1 bagasse sorghum as buffalo diet.

Key Words: Buffalo, CH4 Concentration, Rumen Fermentation Characteristics, Sorghum

Keywords

Buffalo; CH4 Concentration; Rumen Fermentation Characteristics; Sorghum

Full Text:

PDF HTML

References

Baker SK. 1999. Rumen methanogenes and inhibition of methanogenesis. Aust J Agric Res. 50:1293-1298.

Blümmel M, Makkar HPS, Becker K. 1997. The in vitro gas production: A technique revisited. J Anim Phys Nutr. 77:24-34.

Chanthakhoun V, Wanapat M. 2012. The in vitro gas production and ruminal fermentation of various feeds using rumen fluid from swamp buffalo and cattle. Asian J Anim Vet Adv. 7:54-60.

Colombo D, Crovetto GM, Colombini S, Galassi G, Rapetti L. 2007. Nutritive value of different hybrids of sorghum forage determined in vitro. Ital J Anim Sci. 6:289-291.

Cone JW, Van Gelder AH. 1999. Influence of protein fermentation on gas production profiles. Anim Feed Sci Tech. 76:251-264.

[GLP] General Laboratory Procedure. 1966. Department of dairy sciences. Madison (USA): University of Wisconsin.

Getachew G, Blümmel M, Makkar HPS, Becker K. 1998. In vitro gas measuring techniques for assessment of nutritional quality of feeds: a review. Anim Feed Sci Tech. 72:261-281.

Human S. 2013. Pemuliaan sorgum dengan iptek nuklir. [accessed October 24th 2013]. http://www.batan.go.id/ patir/2012/p_03/01_org/pert/pemuliaan/AIN%20Sorgum.pdf

Jančik F, Koukolova V, Homolka P, Haman J. 2011. Comparison of analyses to predict ruminal fibre degradability and indigestible fibre in temperate grass silages. S Afr J Anim Sci. 41:297-308.

Jančik F, Koukolova V, Homolka P. 2010. Ruminal degradability of dry matter and neutral detergent fibre of grasses. Czech J Anim Sci. 55:359-371.

Jayanegara A, Makkar HPS, Becker K. 2009. Emisi metana dan fermentasi rumen in vitro ransum hay yang mengandung tanin murni pada konsentrasi rendah. Media Peternakan. 32:184-194.

Kamal M. 1994. Nutrisi ternak 1. Yogyakarta (Indones): Universitas Gadjah Mada.

Kang S, Wanapat M. 2013. Increasing rumen microbial protein synthesis using cassava chip and urea in swamp buffaloes. Proceedings of the 10th World Buffalo Congress. Phuket (Thailand).

Krishnamoorthy U. 2001. RCA training workshop on in vitro techniques for feed evaluation. Jakarta (Indones): The International Atomic Energy Agency. p. 17.

Kumar R, Kamra DN, Agarwal N, Chaudhary LC. 2007. In vitro methanogenesis and fermentation of feeds containing oil seed cakes with rumen fluid of buffalo. Asian-Aust J Anim Sci. 20:1196-1200.

Luna AC, Diaz DD, Murphy MR, Almeida FAR, Villalobos G, Gutierrez JAO. 2013. Relationships between chemical composition, in vitro dry matter, neutral detergent fiber digestibility and in vitro gas production of corn and sorghum silages. J Anim Vet Adv. 12:1524-1529.

Mattjik AA, Sumertajaya M. 2006. Perancangan percobaan dan aplikasi SAS dan minitab. Jilid I. Edisi ke 2. Bogor (Indones): IPB Press.

McDonald P, Edward RA, Greenhalgh JFD, Morgan CA. 2002. Animal nutrition. 6th ed. New York (USA): Scientific and Tech John Willey & Sons. Inc

Menke KH, Raab L, Salewski A, Steingass H, Fritz D, Schneider W. 1979. The estimation of the digestibility and metabolizable energy content of ruminant feeding stuffs from the gas production when they are incubated with rumen fluid in vitro. J Agr Sci. 93:217-222.

Ørskov ER, Mcdonald I. 1979. The estimation of protein degradability in the rumen from incubation measurements weighted according to the rate of passage, J Agr Sci Camb. 92:499-503.

Pamungkas D, Sevilla CC, Lustria UM. 2006. Changes in rumen ecosystem and feed dry matter degradability of buffalo which received rumen content of cattle through cross inoculation. JITV. 11:24-33.

Parakkasi A. 1999. Ilmu nutrisi dan makanan ternak ruminansia. Jakarta (Indones): Universitas Indonesia.

Salem AZM, Chuan-She Z, Zhi-liang T, Mellado M, Salazar MC, Elghandopur MMMY, Edongo NE. 2013. In vitro Ruminal Gas Production Kinetics of Four Fodder Trees Ensiled With or Without Molasses and Urea. J Integr Agr. 12:1234-1242.

Seshaiah CV, Rao SJ, Reddy YR, Mahendar M, Kumar MK. 2013. Effect of feeding differently processed sweet sorghum bagasse based complete rations on feeding behaviour, milk production and cost economics in graded murrah buffaloes. Buffalo Bull. 32:231-238.

Shahbazi HR, Sadeghi AA, Shawrang P, Raisali G. 2008. Effects of gamma irradiation on ruminal DM and NDF degradation kinetics of alfalfa hay, Pak J Biol Sci. 11:1165-1168.

Sihono, Human S, Indriatama WM, Puspitasari W, Parno, Carkum. 2013. Galur Mutan Sorgum PATIR-1 Berdaya Hasil Biji, Biomasa dan Gula Batang Tinggi serta Galur PATIR-4 Hasil Biji Tinggi Kualitas Baik. Perbaikan Proposal Pelepasan Varietas. Pusat Aplikasi Isotop dan Radiasi. BATAN.

Sirappa MP. 2003. Prospek Pengembangan Sorgum di Indonesia sebagai komoditas alternatif untuk pangan, pakan dan industri. J Litbang Pertanian. 22:133-140.

Su-jiang Z, Chaudhry AS, Ramdani D, Osman A, Xue-feng G, Edwards GR, Cheng L. 2016. Chemical composition and in vitro fermentation characteristics of high sugar forage sorghum as an alternative to forage maize for silage making in Tarim Basin, China. J Integr Agr. 15:175-182.

Van Soest PJ. 1994. Nutritional ecology of the ruminant. 2nd ed. New York (USA): Comstock Publishing Associates, Cornell University Press, Ithaca.

Wanapat M, Kang S, Phesatcha K. 2013. Enhancing buffalo production efficiency through rumen manipulation and nutrition. Buffalo Bull. 32:258-275.

Wanapat M, Pimpa O. 1999. Effect of ruminal NH3-N levels on ruminal fermentaion, purine derivatives, digestibility and rice straw intake in swamp buffaloes. Asian-Aust J Anim Sci. 12:904-907.

Wanapat M, Rowlinson P. 2007. Nutrition and feeding of swamp buffalo: Feed resources and rumen approach. Ital J Anim Sci. 6:67-73.

Warner AC. 1964. Production of volatile fatty acids in the rumen: Methods of measurement. Nutr Abstr Rev. 34:339-352.

Widiawati Y, Winugroho M, Mahyuddin P. 2010. Estimasi produksi gas metana dari rumput dan tanaman leguminosa yang diukur secara in vitro. Prasetyo LH, Natalia L, Iskandar S, Puastuti W, Herawati T, Nurhayati, Anggraeni A, Damayanti R, Darmayanti NLPI, Estuningsih SE, editors. Prosiding Seminar Nasional Teknologi Peternakan dan Veteriner. Bogor (Indones): Pusat Penelitian dan Pengembangan Peternakan. p. 131-136.

Yahaya MS, Kawai M, Takahashi J, Matsuoka S. 2002. The effects of different moisture content and ensiling time on silo degradation of structural carbohydrate of orchad grass. Asian-Aust J Anim Sci. 11:213-217.

Refbacks

  • There are currently no refbacks.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.